Single-molecule detection of biomarker and localized cellular photothermal therapy using an optical microfiber with nanointerface

Li, Hongtao; Huang, Yunyun; Guan, Bai‐Ou; Xiao, Aoxiang; Chen, Pengwei; He, Liangmei; Huang, Yugang; Zhao, Xiaotian; Liang, Lili; Feng, Xinhuan

doi:10.1126/sciadv.aax4659

Cited by 62 publications

(44 citation statements)

References 41 publications

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“…Since the 1/ f -noise-free sensing scheme is compatible with previous enhancement mechanisms based on plasmonic nanostructures 15 , 17 , 31 or surface charges 19 , 30 on the target nanoparticles/molecules, the ultra-low noise optical sensor has a high potential for single-molecule dynamic detection. Furthermore, the CMOS-compatible heterodyne interferometer combined with the microfluidics and the chemical-functionalization technology could be widely applied in the fields of biology, medicine, pharmacology, physics, and environmental science.…”

Section: Resultsmentioning

confidence: 88%

“…Effectively suppressing the low-frequency noise is critical but fundamentally challenging to both electrical and optical transducers, especially to the ultrahigh-sensitivity detection and analysis. For example, in optical evanescent sensors based on resonant structures 8 – 10 and nanowaveguides 11 – 15 , 1/ f noise poses serious difficulties for resolving dynamic signals, such as biomolecule motions, binding, and trapping which are typically charaterized by hertz to kilohertz frequencies 16 – 22 . To date, while much effort has been devoted to suppressing the optical fluctuations in evanescent sensors, such as self-reference mode splitting 23 – 26 , frequency tracking 27 – 30 , and lock-in amplifying 14 , 19 , the 1/ f noise suppression in optical sensors has never been explored.…”

Section: Introductionmentioning

confidence: 99%

“…The ultralow-noise sensor is applied to detect single polystyrene and HIV-1 virus-like particles with the detection limit down to a few attograms. The 1/ f -noise-free sensing scheme, combined with conventional enhancement mechanisms based on plasmonic nanostructures 15 , 17 , 31 or surface charges on the analytes 19 , 30 , can provide a powerful way towards studying single-molecule dynamics in fundamental biophysics research and practical biological applications.…”

Section: Introductionmentioning

confidence: 99%

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1/f-noise-free optical sensing with an integrated heterodyne interferometer

et al. 2021

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Optical evanescent sensors can non-invasively detect unlabeled nanoscale objects in real time with unprecedented sensitivity, enabling a variety of advances in fundamental physics and biological applications. However, the intrinsic low-frequency noise therein with an approximately 1/f-shaped spectral density imposes an ultimate detection limit for monitoring many paramount processes, such as antigen-antibody reactions, cell motions and DNA hybridizations. Here, we propose and demonstrate a 1/f-noise-free optical sensor through an up-converted detection system. Experimentally, in a CMOS-compatible heterodyne interferometer, the sampling noise amplitude is suppressed by two orders of magnitude. It pushes the label-free single-nanoparticle detection limit down to the attogram level without exploiting cavity resonances, plasmonic effects, or surface charges on the analytes. Single polystyrene nanobeads and HIV-1 virus-like particles are detected as a proof-of-concept demonstration for airborne biosensing. Based on integrated waveguide arrays, our devices hold great potentials for multiplexed and rapid sensing of diverse viruses or molecules.

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Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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1/f-noise-free optical sensing with an integrated heterodyne interferometer

et al. 2021

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“…Fig. 9 A schematic diagram of different single tapered (a) SMF [181], (b) SMS [194], (c) SSCS [201], (d) SNCS [205], (e) SHCS [212] fiber structure, (f) SEM image of Nile red coated tapered SCF [204], (g) single molecule detection of biomarker and localized cellular photothermal therapy [193], (h) tapered SNCS for ultrahigh sensitivity detection of hCG with signal amplification of magnetic microspheres [210]. Reprinted with permission from [204], [193] and [210].…”

Section: A a Tapered Sms Fiber Structurementioning

confidence: 99%

“…The tapered SMF has been combined with an MMF to achieve simultaneous measurement of both temperature and RI [187]. A nonadiabatically tapered SMF can be functionalized for bio/chemical sensing, such as anti-gliadin antibodies [188], glucose detection [189], enzyme-linked immunosorbent assay reader [190], carcinoembryonic antigen-related cell adhesion molecules detection [191], microRNA quantification [192], and biomarker detection in a single molecule [193].…”

Section: A a Tapered Sms Fiber Structurementioning

confidence: 99%

Singlemode-Multimode-Singlemode Fiber Structures for Sensing Applications—A Review

Liu

et al. 2021

IEEE Sensors J.

127

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A singlemode-multimode-singlemode (SMS) fiber structure consists of a short section of multimode fiber fusionspliced between two SMS fibers. The mechanism underpinning the operation of an SMS fiber structure is multimode interference and associated self-imaging. SMS structures can be used in a variety of optical fiber systems but are most commonly used as sensors for a variety of parameters, ranging from macro-world measurands such as temperature, strain, vibration, flow rate, RI and humidity to the micro-world with measurands such as proteins, pathogens, DNA, and specific molecules. While traditional SMS structures employ a short section of standard multimode fiber, a large number of structures have been investigated and demonstrated over the last decade involving the replacement of the multimode fiber section with alternatives such as a hollow core fiber or a tapered fiber. The objective of replacing the multimode fiber has most often been to allow sensing of different measurands or to improve sensitivity. In this paper, several different categories of SMS fiber structures, including traditional SMS, modified SMS and tapered SMS fiber structures are discussed with some theoretical underpinning and reviews of a wide variety of sensing examples and recent advances. The paper then summarizes and compares the performances of a variety of sensors which have been published under a number of headings. The paper concludes by considering the challenges faced by SMS based sensing schemes in terms of their deployment in real world applications and discusses possible future developments of SMS fiber sensors.

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Plasmonic Coupling on an Optical Microfiber Surface: Enabling Single‐Molecule and Noninvasive Dopamine Detection

et al. 2023

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Optical fibers can be effective biosensors when employed in early‐stage diagnostic point‐of‐care devices as they can avoid interference from molecules with similar redox potentials. Nevertheless, their sensitivity needs to be improved for real‐world applications, especially for small‐molecule detection. This work demonstrates an optical microfiber biosensor for dopamine (DA) detection based on the DA‐binding‐induced aptamer conformational transitions that occur at plasmonic coupling sites on a double‐amplified nanointerface. The sensor exhibits ultrahigh sensitivity when detecting DA molecules at the single‐molecule level; additionally, this work provides an approach for overcoming optical device sensitivity limits, further extending optical fiber single‐molecule detection to a small molecule range (e.g., DA and metal ions). The selective energy enhancement and signal amplification at the binding sites effectively avoid nonspecific amplification of the whole fiber surface which may lead to false‐positive results. The sensor can detect single‐molecule DA signals in body‐fluids. It can detect the released extracellular DA levels and monitor the DA oxidation process. An appropriate aptamer replacement allows the sensor to be used for the detection of other target small molecules and ions at the single‐molecule level. This technology offers alternative opportunities for developing noninvasive early‐stage diagnostic point‐of‐care devices and flexible single‐molecule detection techniques in theoretical research.

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Single-molecule detection of biomarker and localized cellular photothermal therapy using an optical microfiber with nanointerface

Cited by 62 publications

References 41 publications

1/f-noise-free optical sensing with an integrated heterodyne interferometer

1/f-noise-free optical sensing with an integrated heterodyne interferometer

Singlemode-Multimode-Singlemode Fiber Structures for Sensing Applications—A Review

Plasmonic Coupling on an Optical Microfiber Surface: Enabling Single‐Molecule and Noninvasive Dopamine Detection

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