Single molecule imaging of H-Ras activation in living cells

Paradigm Shift of the Molecular Dynamics Concept in the Cell Membrane: High‐Speed Single‐Molecule Tracking Revealed the Partitioning of the Cell Membrane

“…These methods include single-fluorescent molecule tracking (SFMT) with the use of fluorescent probes, and single-particle tracking (SPT) using colloidal gold probes with a diameter of 20 or 40 nm Figure 19. [1,[6][7][8]14,16,18,25,29,34,36,50,59,61,65,[67][68][69]. The plasma membrane specimen was rapidly frozen on a copper block precooled in liquid helium, deeply etched to remove excess ice, and platinum-carbon replicated.…”

“…The plasma membrane specimen was rapidly frozen on a copper block precooled in liquid helium, deeply etched to remove excess ice, and platinum-carbon replicated. Furthermore, not only the movement of single molecules, but also the activation of cellular signaling molecules, including the small GTP binding proteins (G proteins) H-and K-Ras, has been tracked at the level of single molecules in the living cell membrane [36]. The striped band-ing patterns with the 5.5-nm periodicity on individual filaments (see the inset) are characteristic of actin filaments.…”