Single-Molecule Surface-Enhanced Raman Spectroscopy

Ru, Eric C. Le; Etchegoin, P.

doi:10.1146/annurev-physchem-032511-143757

Cited by 673 publications

(504 citation statements)

References 155 publications

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“…1a, blue spectrum). A pre-resonance enhancement factor of 2×10 5 can be achieved for this conjugated C=C when compared to C-O in methanol, a magnitude comparable to previous reports for resonance Raman spectroscopy 18 . The epr-SRS detection limit of ATTO740 by targeting this peak is determined to be 250 nM with 1-ms time constant (Fig.…”

supporting

confidence: 84%

Super-multiplex vibrational imaging

Chen

Shi

et al. 2017

Nature

433

452

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The ability to directly visualize a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have been used successfully to explore structural-functional relationships in nervous systems, profile RNA in situ, reveal tumor microenvironment heterogeneity or study dynamic macromolecular assembly1–4, it remains challenging to image many species with high selectivity and sensitivity under biological conditions. For instance, fluorescence microscopy faces a “color barrier” due to the intrinsically broad (~1500 cm−1) and featureless nature of fluorescence spectra5 that limits the number of resolvable colors to 2 to 5 (or 7-9 if using complicated instrumentation and analysis)6–8. Spontaneous Raman microscopy probes vibrational transitions with much narrower resonances (peak width ~10 cm−1) and thus doesn’t suffer this problem, but its feeble signals make many demanding bio-imaging applications impossible. And while surface-enhanced Raman scattering offers remarkable sensitivity and multiplicity, it cannot be readily used to quantitatively image specific molecular targets inside live cells9. Here we show that carrying out stimulated Raman scattering under electronic pre-resonance conditions (epr-SRS) enables imaging with exquisite vibrational selectivity and sensitivity (down to 250 nM with 1-ms) in living cells. We also create a palette of triple-bond-conjugated near-infrared dyes that each display a single epr-SRS peak in the cell-silent spectral window, and that with available fluorescent probes give 24 resolvable colors with potential for further expansion. Proof-of-principle experiments on neuronal co-cultures and brain tissues reveal cell-type dependent heterogeneities in DNA and protein metabolism under physiological and pathological conditions, underscoring the potential of this super-multiplex optical imaging approach for untangling intricate interactions in complex biological systems.

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supporting

confidence: 84%

Super-multiplex vibrational imaging

Chen

Shi

et al. 2017

Nature

433

452

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“…Cues to the conditions under which SM-SERS could be observed are being increasingly well understood, and have been discussed and reviewed in several works [21][22][23][24]. Herein, only the basic properties and the most important aspects and features will be summarized and highlighted.…”

Section: Basics Of Single-molecule Surface-enhancedmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy at single-molecule scale and its implications in biology

Wang

Irudayaraj

2013

Phil. Trans. R. Soc. B

106

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Single-molecule (SM) spectroscopy has been an exciting area of research offering significant promise and hope in the field of sensor development to detect targets at ultra-low levels down to SM resolution. To the experts and developers in the field of surface-enhanced Raman spectroscopy (SERS), this has often been a challenge and a significant opportunity for exploration. Needless to say, the opportunities and excitement of this multidisciplinary area impacts span the fields of physics, chemistry and engineering, along with a significant thrust in applications constituting areas in medicine, biology, environment and agriculture among others. In this review, we will attempt to provide a quick snapshot of the basics of SM-SERS, nanostructures and devices that can enable SM Raman measurement. We will conclude with a discussion on SERS implications in biomedical sciences.

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“…The discovery of surface enhanced Raman spectroscopy (SERS) in 1974 by Fleischman, Hendra, and McQuillan [4] has greatly fostered the application of Raman spectroscopy. When a molecule is adsorbed at or near a roughened metal surface, such as Ag or Au, the averaged Raman signal could be enhanced by a factor of six to eight orders of magnitude over an ordinary Raman signal [1][2][3][4].…”

Section: Qing-hua Xumentioning

confidence: 99%

SERS under magnetic control

2012

Annalen der Physik

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Single-Molecule Surface-Enhanced Raman Spectroscopy

Cited by 673 publications

References 155 publications

Super-multiplex vibrational imaging

Super-multiplex vibrational imaging

Surface-enhanced Raman spectroscopy at single-molecule scale and its implications in biology

SERS under magnetic control

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