Single mutation converts mild pathotype of the Pepino mosaic virus into necrotic one

Hasiów‐Jaroszewska, Beata; Borodynko, N.; Jackowiak, Paulina; Figlerowicz, Marek; Pospieszny, H.

doi:10.1016/j.virusres.2011.04.008

Cited by 44 publications

(49 citation statements)

References 19 publications

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“…Non-random mutation in position TGB3/K67E, and both mutations in positions CP/E155K and CP/D166G, have previously been described as a determinant of disease symptoms [43, 45]. These mutations were first identified on tomato plants infected with the CH2 strain and still are present in the virus population.…”

Section: Discussionmentioning

confidence: 99%

“…These mutations were first identified on tomato plants infected with the CH2 strain and still are present in the virus population. They have different effects on development of symptoms, virus replication and accumulation [43, 45, 57, 58]. Overexpression of both TGB3 gene and the polymerase domain (POL) of the RdRp gene plays an important role in necrosis induction.…”

Section: Discussionmentioning

confidence: 99%

“…Twenty-two were randomly chosen and three were previously described [43, 45]. Five of these mutations (three random and two previously described as a determinant of yellowing symptoms) were also introduced into the genome of PepMV-P11 (Table 1 and Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Although the nucleotide sequence identity between isolates closely related to each other ranges 99%, they cause diverse disease symptoms. It has been previously shown that single nucleotide substitutions A199G (TGB3/K67E) in TGB3 and G463A (CP/E155K) and A497G (CP/D166G) in CP genes play a role in the development of necrotic and yellowing symptoms, respectively [43–45]. Furthermore, a high number of genetic variants were identified during long-term experimental evolution (20 serial passages in five evolutionary lineages) of PepMV in different hosts [46].…”

Section: Introductionmentioning

confidence: 99%

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Strain-dependent mutational effects for Pepino mosaic virus in a natural host

et al. 2017

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Background Pepino mosaic virus (PepMV) is an emerging plant pathogen that infects tomatoes worldwide. Understanding the factors that influence its evolutionary success is essential for developing new control strategies that may be more robust against the evolution of new viral strains. One of these evolutionary factors is the distribution of mutational fitness effect (DMFE), that is, the fraction of mutations that are lethal, deleterious, neutral, and beneficial on a given viral strain and host species. The goal of this study was to characterize the DMFE of introduced nonsynonymous mutations on a mild isolate of PepMV from the Chilean 2 strain (PepMV-P22). Additionally, we also explored whether the fitness effect of a given mutation depends on the gene where it appears or on epistatic interactions with the genetic background. To address this latter possibility, a subset of mutations were also introduced in a mild isolate of the European strain (PepMV-P11) and the fitness of the resulting clones measured.ResultsA collection of 25 PepMV clones each containing a single nucleotide nonsynonymous substitution was created by site-directed mutagenesis and the fitness of each mutant was determined. PepMV-P22 genome showed a high degree of robustness against point mutations, with 80% of mutations being either neutral or even beneficial and only 20% being deleterious or lethal. We found that the effect of mutations strongly depended on the gene in which they were introduced. Mutations with the largest average beneficial effects were those affecting the RdRp gene, in contrast to mutations affecting TGB1 and CP genes, for which the average effects were deleterious. Moreover, significant epistatic interactions were observed between nonsynonymous mutations and the genetic background, meaning that the effect of a given nucleotide substitution on a particular genomic context cannot be predicted by knowing its effect in a different one.ConclusionsOur results indicated that PepMV genome has a surprisingly high robustness against mutations. We also found that fitness consequences of a given mutation differ between the two strains analyzed. This discovery suggests that the strength of selection, and thus the rates of evolution, vary among PepMV strains.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Strain-dependent mutational effects for Pepino mosaic virus in a natural host

et al. 2017

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“…Recently, it was proved that one single mutation in TGBp3 protein converts a mild pathotype of CH2 genotype into a necrotic one. This is the only association reported between particular amino acid sites and symptomatology [6]. However, our preliminary results with isolates from the EU genotype showed that we are dealing with a universal mechanism of variability.…”

Section: Introductionmentioning

confidence: 65%

Tridimensional model structure and patterns of molecular evolution of Pepino mosaic virus TGBp3 protein

Hasiów‐Jaroszewska

Czerwoniec

Pospieszny

et al. 2011

Virol J

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BackgroundPepino mosaic virus (PepMV) is considered one of the most dangerous pathogens infecting tomatoes worldwide. The virus is highly diverse and four distinct genotypes, as well as inter-strain recombinants, have already been described. The isolates display a wide range on symptoms on infected plant species, ranging from mild mosaic to severe necrosis. However, little is known about the mechanisms and pattern of PepMV molecular evolution and about the role of individual proteins in host-pathogen interactions.MethodsThe nucleotide sequences of the triple gene block 3 (TGB3) from PepMV isolates varying in symptomatology and geographic origin have been analyzed. The modes and patterns of molecular evolution of the TGBp3 protein were investigated by evaluating the selective constraints to which particular amino acid residues have been subjected during the course of diversification. The tridimensional structure of TGBp3 protein has been modeled de novo using the Rosetta algorithm. The correlation between symptoms development and location of specific amino acids residues was analyzed.ResultsThe results have shown that TGBp3 has been evolving mainly under the action of purifying selection operating on several amino acid sites, thus highlighting its functional role during PepMV infection. Interestingly, amino acid 67, which has been previously shown to be a necrosis determinant, was found to be under positive selection.ConclusionsIdentification of diverse selection events in TGB3p3 will help unraveling its biological functions and is essential to an understanding of the evolutionary constraints exerted on the Potexvirus genome. The estimated tridimensional structure of TGBp3 will serve as a platform for further sequence, structural and function analysis and will stimulate new experimental advances.

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Development and optimization of a pepino mosaic virus-based vector for rapid expression of heterologous proteins in plants

Abrahamian

Hammond

2021

Appl Microbiol Biotechnol

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Single mutation converts mild pathotype of the Pepino mosaic virus into necrotic one

Cited by 44 publications

References 19 publications

Strain-dependent mutational effects for Pepino mosaic virus in a natural host

Strain-dependent mutational effects for Pepino mosaic virus in a natural host

Tridimensional model structure and patterns of molecular evolution of Pepino mosaic virus TGBp3 protein

Development and optimization of a pepino mosaic virus-based vector for rapid expression of heterologous proteins in plants

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