Single-Nucleotide Polymorphism at CYP27B1-1260, but Not VDR Taq I, Is Possibly Associated with Persistent Hepatitis B Virus Infection

Abstract

Background: Vitamin D, beyond its role in calcium and bone metabolism, exhibits immunomodulatory effects on innate and adaptive immune pathways and is suggestively related to liver diseases. Objective: This study investigated the association of single-nucleotide polymorphisms in genes involved in vitamin D functions with hepatitis B virus (HBV) infection. Methods: Five hundred Chinese Han subjects, including 274 chronic HBV patients, 68 HBV infection resolvers, and 158 healthy controls without HBV infection, were studied. The CYP27B1-1260 promoter and the VDR Taq I polymorphisms were genotyped by polymerase chain reactionrestriction fragment length polymorphism. Results: Although there was no difference between HBV patients and healthy controls, HBV patients and healthy controls had a higher frequency of the CYP27B1-1260 genotype CC (15.0% vs. 2.9%, p = 0.004 and 13.3% vs. 2.9%, p = 0.006, respectively) and allele C (38.3% vs. 25.7%, p = 0.006 and 39.2% vs. 25.7%, p = 0.006, respectively) compared with resolvers. The genotype and allele frequencies of the VDR Taq I polymorphism had no difference between patients, resolvers, and healthy controls. Conclusion: These results suggest that the CYP27B1-1260 promoter polymorphism is possibly associated with the persistence, but not susceptibility to HBV infection in Chinese HBV patients, and that the VDR Taq I polymorphism is not suggested to be related to chronic HBV infection.

Vitamin D Receptor polymorphisms and risk of enveloped virus infection: A meta-analysis

“…9 studies were eligible to be included in the meta-analysis (Alagarasu et al, 2012;Bellamy et al, 1999;Cohen, 2004;Janssen et al, 2007;Kresfelder et al, 2011;de la Torre et al, 2008;D. E. Roth et al, 2008;Suneetha et al, 2006;Zhu et al, 2012). Characteristics of the studies fitting the inclusion criteria are reported in Table 1 and FokI and TaqI and were genotyped in 6 of the selected studies.…”

Association of Vitamin-D-Related Genetic Variations and Treatment Response to Pegylated Interferon in Patients with Chronic Hepatitis B

“…Moreover, common single nucleotide polymorphisms (SNPs) in vitamin-D-related genes, including vitamin D receptor (VDR), vitamin D binding protein (DBP) and enzymes that initiate vitamin D formation such as 1-a-hydroxylase (encoded by CYP27B1), are shown to be associated with clinical outcome and response to PEG-IFN-based therapy in patients with chronic HCV infection [5][6][7][8][9]. Among HBV-infected individuals, recent studies have suggested a possible role of these SNPs in viral persistence and HCC development [10,11]. In addition, it has recently been demonstrated that a variant of CYP27B1 is associated with high probability of response to PEG-IFN therapy in an Italian cohort of HBeAg-negative CHB [12].…”

Role of CYP27B1+2838 promoter polymorphism in the treatment of chronic hepatitis B HBeAg negative with PEG‐interferon

“…The polymorphisms within the vitamin D receptor (VDR) and 1‐ α ‐hydroxylase (CYP27B1) were recently associated with virological response to PEG‐IFN and ribavirin in HCV treatment , with onset of oral cancer , type 1 diabetes and autoimmunity disorders ; moreover, a possible role in the natural course of HBV infection was recently reported by Zhu et al . , but no data were available about the treatment with PEG‐IFN in HBV.…”