Single nucleotide polymorphisms and haplotype of MD‐1 gene associated with high serum IgE phenotype with mite‐sensitive allergy in Taiwanese children

Wang, J. Y.; Lin, Cherry Guan Ju; Hsiao, Yu‐Ping; Liou, Ya-Huei; Wu, Lei

doi:10.1111/j.1744-313x.2007.00711.x

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…CD14 is an extracellular component of TLR4 which functions as a co-receptor required by the activation of TLR4 induced by lipopolysaccharide (LPS) [23]. MD-1, also known as Ly86, interacting with RP105, plays an important role in the TLR4 signaling pathway [38,39]. Ticam2 physically bridges TLR4 and TICAM-1 and functionally transmits LPS-TLR4 signaling to TICAM-1, which in turn activates IRF-3 [40].…”

Section: Discussionmentioning

confidence: 99%

Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone

Fang

Wang

Ishrat

et al. 2011

Journal of Neuroinflammation

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BackgroundTraumatic brain injury (TBI) causes acute inflammatory responses that result in an enduring cascade of secondary neuronal loss and behavioral impairments. It has been reported that progesterone (PROG) can inhibit the increase of some inflammatory cytokines and inflammation-related factors induced by TBI. Toll-like receptors (TLRs) play a critical role in the induction and regulation of immune/inflammatory responses. Therefore, in the present study, we examined the genomic profiles of TLR-mediated pathways in traumatically injured brain and PROG's effects on these genes.MethodsBilateral cortical impact injury to the medial frontal cortex was induced in C57BL/6J mice. PROG was injected (i.p., 16 mg/kg body weight) at 1 and 6 h after surgery. Twenty-four hours post-surgery, mice were killed and peri-contusional brain tissue was harvested for genomic detection and protein measurement. RT-PCR arrays were used to measure the mRNA of 84 genes in TLR-mediated pathways. Western blot, ELISA and immunohistochemistry were used to confirm the protein expression of genes of interest.ResultsWe found that 2 TLRs (TLR1 and 2), 5 adaptor/interacting proteins (CD14, MD-1, HSPA1a, PGRP and Ticam2) and 13 target genes (Ccl2, Csf3, IL1a, IL1b, IL1r1, IL6, IL-10, TNFa, Tnfrsf1a, Cebpb, Clec4e, Ptgs2 and Cxcl10) were significantly up-regulated after injury. Administration of PROG significantly down-regulated three of the 13 increased target genes after TBI (Ccl-2, IL-1b and Cxcl-10), but did not inhibit the expression of any of the detected TLRs and adaptor/interacting proteins. Rather, PROG up-regulated the expression of one TLR (TLR9), 5 adaptor/interacting proteins, 5 effectors and 10 downstream target genes. We confirmed that Ccl-2, Cxcl-10, TLR2 and TLR9 proteins were expressed in brain tissue, a finding consistent with our observations of mRNA expression.ConclusionThe results demonstrate that TBI can increase gene expression in TLR-mediated pathways. PROG does not down-regulate the increased TLRs or their adaptor proteins in traumatically injured brain. Reduction of the observed inflammatory cytokines by PROG does not appear to be the result of inhibiting TLRs or their adaptors in the acute stage of TBI.

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Section: Discussionmentioning

confidence: 99%

Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone

Fang

Wang

Ishrat

et al. 2011

Journal of Neuroinflammation

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“… 14 , 15 Recently, the promoter polymorphism of MD-1 has been reported to be associated with mite-sensitive allergies possibly to be a susceptible gene for asthma. 16 However, the role of MD-2 has yet to be elucidated. Der p 2 has structural homology with MD-2 protein, 17 the lipopolysaccharide (LPS)-binding component of the TLR-4 signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

Association of Single Nucleotide Polymorphisms in the MD-2 Gene Promoter Region With Der p 2 Allergy

Liao

Chang

et al. 2015

Allergy Asthma Immunol Res

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PurposeSensitization to house dust mite (Dermatophagoides pteronyssinus) is a considerable risk factor for the progression of allergic disease. The group 2 allergen from Dermatophagoides pteronyssinus, Der p 2, is considered a major one in patients with specific immunoglobulin E (IgE) to Der p 2. Der p 2 has structural homology with myeloid differentiation 2 (MD-2), which is involved in the lipopolysaccharide-binding component of the Toll-like receptor 4 signaling pathway and the development of inflammation. The aim of this study was to examine the genetic association of single nucleotide polymorphisms (SNPs) in the promoter region of MD-2 with Der p 2-sensitive allergy.MethodsWe investigated associations between cohort's characteristics, including 280 allergic and 80 healthy subjects by examining total IgE, eosinophils, D. pteronyssinus-specific IgE, Der p 2-specific IgE, the number of IgE-producing B cells induced by Der p 2, and the odds ratio of allergic symptoms.ResultsBased on the 1,000 genome project data, the minor allele frequencies of the rs1809441 and rs1809442 are 0.467 and 0.474, respectively. However, the correlation of linkage disequilibrium (LD) between these 2 SNPs is D'=1, the genotype frequencies of the 2 MD-2 (LY96) SNPs (rs1809441 and rs1809442) that are located nearby were significantly different between allergic and health subjects: the TT genotype of rs1809441 and the GG genotype of rs1809442 were more frequent in allergic subjects than in healthy subjects (16.1% vs 2.5% in both genotypes). The allergic patients with these genotypes exhibited significantly higher levels of D. pteronyssinus-specific IgE and Der p 2-specific Ig E, and a larger number of Der p 2-activated B cells. In addition, these 2 SNPs in the MD-2 promoter region were significantly associated with the prevalence of nasal, skin, and asthmatic allergic symptoms.ConclusionsOur results indicated that 2 SNPs in the MD-2 promoter region were significantly associated with Der p 2-specific Ig E, and thereby suggest that these SNPs may play a major role in susceptibility to Der p 2-triggered immune responses in a Taiwanese population.

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MD-1 deficiency attenuates dextran sodium sulfate (DSS)-induced colitis through modulating the function of colonic lamina propria dendritic cells

Pan

Zhang

et al. 2016

Molecular Immunology

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Single nucleotide polymorphisms and haplotype of MD‐1 gene associated with high serum IgE phenotype with mite‐sensitive allergy in Taiwanese children

Cited by 7 publications

References 22 publications

Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone

Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone

Association of Single Nucleotide Polymorphisms in the MD-2 Gene Promoter Region With Der p 2 Allergy

MD-1 deficiency attenuates dextran sodium sulfate (DSS)-induced colitis through modulating the function of colonic lamina propria dendritic cells

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