Single Nucleotide Polymorphisms in miR-149 (rs2292832) and miR-101-1 (rs7536540) Are Not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection

Abstract

MicroRNAs directly and indirectly influence many biological processes such as apoptosis, cell maintenance, and immune responses, impacting on tumor genesis and metastasis. They modulate gene expression at the posttranscriptional level and are associated with progression of liver disease. Hepatocellular carcinoma (HCC) is a cancer which mostly occurs in males. There are many factors affect HCC development, for example, hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV), co-infection, environmental factors including alcohol, aflatoxin consumption and host-related factors such as age, gender immune response, microRNA and single nucleotide polymorphisms (SNPs). Chronic infection with the hepatitis B virus is the major factor leading to HCC progression since it causes the liver injury. At present, there are many reports regarding the association of SNPs on miRNAs and the HCC progression. In this research, we investigated the role of miR-149 (rs2292832) and miR-101-1 (rs7536540) with HCC progression in Thai population. The study included 289 Thai subjects including 104 HCC patients, 90 patients with chronic hepatitis B virus infection (CHB) and 95 healthy control subjects. The allele and genotype of rs2292832 and rs7536540 polymorphisms were determined by TaqMan real-time PCR assay. Our results revealed no significant association between miR-149 (rs2292832) and miR-101-1 (rs7536540) and the risk of HCC in our Thai population. However, this research is the first study of miR-149 (rs2292832) and miR-101-1 (rs7536540) in HCC in Thai populations and the results need to be confirmed with a larger population.

Association of mir-196a-2 rs11614913 and mir-149 rs2292832 Polymorphisms With Risk of Cancer: An Updated Meta-Analysis

“… Forest plot of the original (Left) and HWD-adjusted (Right) meta-analysis of cancer risk associated with mir-149 rs2292832 in subgroup of studies which used a non-RFLP genotyping method assuming the heterozygote model. In the right plot, HWD-adjusted log ORs and 95%CI were used for the underlined studies (i.e., He, 2018; Kontorovich et al, 2010 ; Pratedrat et al, 2015 ; Jiang et al, 2016 ). …”

MicroRNA-Related Polymorphisms in Infectious Diseases—Tiny Changes With a Huge Impact on Viral Infections and Potential Clinical Applications

“…The influence of miR-149 C/T SNP (rs2292832) and miR-101-1 C/G/T SNP (rs7536540) on the risk of HCC in Thai population was evaluated by Pratedrat et al ( 81 ), in a study including 95 healthy controls, 90 chronic HBV-infected individuals, and 104 HCC patients. However, no statistically significant association was found ( 81 ).…”

“…The influence of miR-149 C/T SNP (rs2292832) and miR-101-1 C/G/T SNP (rs7536540) on the risk of HCC in Thai population was evaluated by Pratedrat et al ( 81 ), in a study including 95 healthy controls, 90 chronic HBV-infected individuals, and 104 HCC patients. However, no statistically significant association was found ( 81 ). In addition to miR-101-1 rs7536540, the influence of the following variants on clinical outcome of HBV infection was investigated in Korean individuals ( 77 ): miR-101-2 C/T SNP (rs17803780), miR-101-2 C/T SNP (rs12375841), and miR-338 C/T SNP (rs62073058).…”

The polymorphism of rs2227513 can affect the expression of IL-22 and the proliferation of FLS, which leads to the progress of rheumatoid arthritis

“…MiR-101 rs7536540 may influence the expression of miR-101 and another polymorphism, rs2227513, to affect the expression of IL-22 [16][17][18]. In this study, we investigated the combined effect of these two polymorphisms on the expression of IL-22 and the proliferation of FLS as well as their association with the severity of RA.…”