Single Particle Mass Spectrometry–Bioaerosol Analysis by                    MALDI MS

Kleefsman, W.A.; Stowers, M.A.; Verheijen, P.J.T.; Marijnissen, J.C.M.

doi:10.14356/kona.2008018

Cited by 19 publications

(13 citation statements)

References 24 publications

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“…The analysis of biological aerosols has been no different, and many different MS techniques have been employed for PBAP characterisation. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) has been successfully applied in several PBAP studies (Kim et al, 2005;van Wuijckhuijse et al, 2005;Kleefsman et al, 2008;Russell, 2009). Parker et al (2000) developed an instrument that utilised ion-trap mass spectrometry for detection and analysis of pollen, bacteria and other aerosol types.…”

Section: Light Detection and Ranging (Lidar) And Remote Sensingmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,191

1,114

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A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.

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Section: Light Detection and Ranging (Lidar) And Remote Sensingmentioning

confidence: 99%

Primary biological aerosol particles in the atmosphere: a review

Després¹,

Huffman

Burrows³

et al. 2012

Tellus B: Chemical and Physical Meteorology

1,191

1,114

View full text Add to dashboard Cite

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“…In combination with an impactor sampler, bioaerosols directly deposited on a target have been analysed [92]. Using instrumental improvements it is even possible to obtain a mass spectrum of a single airborne particle allowing on-line measurements and analysis without the need of prior cultivation [93].…”

Section: Polymerase Chain Reaction (Pcr) -Pcr Techniquementioning

confidence: 99%

Bioaerosols in Indoor Environment - A Review with Special Reference to Residential and Occupational Locations

Brandl¹

2011

TOEBMJ

177

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Bioaerosols such as bacterial and fungal cells and their spores are -along with non-biological particles -part of indoor airborne particulate matter and have been related since a long time to health issues of human beings as well as flora, and fauna. To identify the different risks and to establish exposure thresholds, microbiology of air samples from a series of indoor environments must be characterized, i.e. the different microorganisms (bacteria and fungi) must be identified and quantified. This review discusses the techniques of air sampling and sample analysis. In addition, a literature study has been performed regarding the levels of these microorganisms in various indoor occupational (e.g., schools, offices, hospitals, museums) and dwelling environments. These results will provide a significant scientific basis for indoor air quality control and help in elaborating risk prevention programs for workers and dwellers. This review shall contribute to the knowledge of identification and quantification of airborne microbial constituents in various indoor environments. Combining the indoor microbial load data with data from studies focusing on health effects caused by inhalation of specific airborne microorganisms will allow the evaluation of various risks to which inhabitants are exposed. Bioaerosols in Indoor Environment -A Review with Special Reference to Residential and Occupational LocationsJyotshna Mandal and Helmut Brandl* Institute of Evolutionary Biology and Environmental Studies, University of Zurich, SwitzerlandAbstract: Bioaerosols such as bacterial and fungal cells and their spores are -along with non-biological particles -part of indoor airborne particulate matter and have been related since a long time to health issues of human beings as well as flora, and fauna. To identify the different risks and to establish exposure thresholds, microbiology of air samples from a series of indoor environments must be characterized, i.e. the different microorganisms (bacteria and fungi) must be identified and quantified. This review discusses the techniques of air sampling and sample analysis. In addition, a literature study has been performed regarding the levels of these microorganisms in various indoor occupational (e.g., schools, offices, hospitals, museums) and dwelling environments. These results will provide a significant scientific basis for indoor air quality control and help in elaborating risk prevention programs for workers and dwellers. This review shall contribute to the knowledge of identification and quantification of airborne microbial constituents in various indoor environments. Combining the indoor microbial load data with data from studies focusing on health effects caused by inhalation of specific airborne microorganisms will allow the evaluation of various risks to which inhabitants are exposed.

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“…These differences derived from culture media can be used to track the bacterial growing environment and source. Thus, the present method may have the potential for the rapid bioaerosol detection, which is a new approach compared with the single particle aerosol MALDI that has been used as a tool to achieve the online bioaerosol detection [33,34].…”

Section: Resultsmentioning

confidence: 99%

Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry

Shu

Liang

et al. 2012

International Journal of Mass Spectrometry

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a b s t r a c tThe rapid identification of bacteria is of increasing interest in food, biosafety, and clinical analysis. In this paper, we report a technique for rapid lipid profiling of bacteria by online droplet-deposited MALDI mass spectrometry. In the experiment, lipids in bacterial cells (Escherichia coli, Salmonella, and Bacillus subtilis) are extracted with the mixture of chloroform/methanol via ultrasonication. The extracts are nebulized into suspended droplets and then instantly analyzed with an online droplet-deposited MALDI-TOF mass spectrometer. The lipid profiles of the three bacteria cultured in nutrient broth and LB (Lysogeny Broth) medium are obtained. The lipids phosphatidylethanolamine (PE), diglycosyl diacylglycerol (DGDG), and phosphatidylglycerol (PG) are observed in the MALDI-TOF mass spectra. The MALDI-TOF mass spectrum of each bacterial extract shows a characteristic profile. The experimental results demonstrate that the present technique is capable of conducting rapid lipid analysis and has the potential for rapid bioaerosol detection.

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Single Particle Mass Spectrometry–Bioaerosol Analysis by MALDI MS

Cited by 19 publications

References 24 publications

Primary biological aerosol particles in the atmosphere: a review

Primary biological aerosol particles in the atmosphere: a review

Bioaerosols in Indoor Environment - A Review with Special Reference to Residential and Occupational Locations

Rapid lipid profiling of bacteria by online MALDI-TOF mass spectrometry

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