Single scattering by red blood cells

Hammer, Martin; Schweitzer, Dietrich; Michel, Bernhard; Thamm, E.; Kolb, Achim

doi:10.1364/ao.37.007410

Cited by 209 publications

(155 citation statements)

References 18 publications

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“…The measured indices of refraction of HbO 2 and Hb solutions at 800 nm are 1:392 0:001 and 1:388 0:002, respectively, average SD of 1000 measurements. These values are in very close agreement with values reported in the literature, recalculated at 800 nm when appropriate [13][14][15][16][17]. In Ref.…”

supporting

confidence: 90%

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Oxygen Saturation-Dependent Absorption and Scattering of Blood

et al. 2004

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supporting

confidence: 90%

“…Other, possibly more accurate scattering theories (e.g., Ref. [17,23]) also take the complex refractive index as input, and it is its SO 2 dependence that determines the SO 2 dependent scattering properties. Our method therefore provides a template for accurate evaluation of the blood scattering properties.…”

Section: P H Y S I C a L R E V I E W L E T T E R Smentioning

confidence: 99%

Oxygen Saturation-Dependent Absorption and Scattering of Blood

et al. 2004

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“…Therefore, our work was first focused on assessing the HDFM protocol for red blood cell (RBC) imaging and developing the spectral library for healthy human RBCs. The morphology and optical characteristics of erythrocytes have been widely studied by numerous research groups [8,23,27]. Spectral characterization and information obtained from RBC can be further applied to the evaluation of health and disease conditions, since pathological status can alter the structure and molecular content of RBC [4].…”

Section: Introductionmentioning

confidence: 99%

Building up spectral libraries for mapping erythrocytes by hyperspectral dark field microscopy

Conti

Scanferlato

Louka³

et al. 2016

BSI

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Abstract. BACKGROUND:Red blood cells (RBC) are obtained by non-invasive methods and widely used for diagnostic tests of health status. Hyperspectral Dark Field Microscopy (HDFM) is a promising technique for nanoscale bio imaging and spectral analysis without additional sample preparation. OBJECTIVE: Develop a protocol for human RBC characterization by HDFM, checking the feasibility of a reference spectral library that can image and afford a new comprehensive descriptor of RBC status. METHOD: A step-by-step protocol for HDFM measurement of human RBC was for the first time established using 5 µl of EDTA-treated whole blood from healthy adults (n = 30). Hyperspectral characteristics of solutions/suspensions at biological concentrations of phospholipids, hemoglobin, spectrin, cholesterol and protoporphyrin IX, as the most relevant RBC components, were also determined. RESULTS: A library made of 8 end-member spectra and classification of their spectral distribution carried out by Single Angle Mapper (SAM) were determined, furnishing a comprehensive mapping and descriptor of healthy human RBC. The spectra of single components allowed some of the RBC spectral bands to be attributed. CONCLUSIONS: This work reports for the first time the hyperspectral optical imaging of the human RBC by a library made of 8 scattering spectra, whose spectral signatures are compared with those of the main RBC molecular components. The percent distribution of the spectral end-members was also achieved, thus giving for the first time the HDFM mapping of human healthy RBCs. The protocol developed herein allows the clinical potential of hyperspectral imaging to be developed for the use of RBC mapping in health and disease.

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“…The approximate theory of light scattering by large spherical particles was first proposed by Van de Hulst [23] and can be extended to large particles of an arbitrary shape. Early and later studies of light scattering by cells [23][24][25][26][27][28][29] found good agreement with Mie theory when the cell was approximated as denser sphere imbedded into a larger and softer sphere. The sizes of the spheres corresponded to the average sizes of the cell nuclei and cell, respectively.…”

Section: Light Scattering From Cells and Organellesmentioning

confidence: 77%

Quantum squeezed light for probing mitochondrial membranes and study of neuroprotectants.

Gourley¹,

Copeland²,

McDonald³

et al. 2005

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We report a new nanolaser technique for measuring characteristics of human mitochondria. Because mitochondria are so small, it has been difficult to study large populations using standard light microscope or flow cytometry techniques. We recently discovered a nano-optical transduction method for high-speed analysis of submicron organelles that is well suited to mitochondrial studies. This ultrasensitive detection technique uses nano-squeezing of light into photon modes imposed by the ultrasmall organelle dimensions in a semiconductor biocavity laser. In this paper, we use the method to study the lasing spectra of normal and diseased mitochondria. We find that the diseased mitochondria exhibit larger physical diameter and standard deviation. This morphological differences are also revealed in the lasing spectra. The diseased specimens have a larger spectral linewidth than the normal, and have more variability in their statistical distributions. 4This page left intentionally blank 5

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Single scattering by red blood cells

Cited by 209 publications

References 18 publications

Oxygen Saturation-Dependent Absorption and Scattering of Blood

Oxygen Saturation-Dependent Absorption and Scattering of Blood

Building up spectral libraries for mapping erythrocytes by hyperspectral dark field microscopy

Quantum squeezed light for probing mitochondrial membranes and study of neuroprotectants.

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