Single Sodium Pyruvate Ingestion Modifies Blood Acid-Base Status and Post-Exercise Lactate Concentration in Humans

Abstract

This study examined the effect of a single sodium pyruvate ingestion on a blood acid-base status and exercise metabolism markers. Nine active, but non-specifically trained, male subjects participated in the double-blind, placebo-controlled, crossover study. One hour prior to the exercise, subjects ingested either 0.1 g·kg−1 of body mass of a sodium pyruvate or placebo. The capillary blood samples were obtained at rest, 60 min after ingestion, and then three and 15 min after completing the workout protocol to analyze acid-base status and lactate, pyruvate, alanine, glucose concentrations. The pulmonary gas exchange, minute ventilation and the heart rate were measured during the exercise at a constant power output, corresponding to ~90% O2max. The blood pH, bicarbonate and the base excess were significantly higher after sodium pyruvate ingestion than in the placebo trial. The blood lactate concentration was not different after the ingestion, but the post-exercise was significantly higher in the pyruvate trial (12.9 ± 0.9 mM) than in the placebo trial (10.6 ± 0.3 mM, p < 0.05) and remained elevated (nonsignificant) after 15 min of recovery. The blood pyruvate, alanine and glucose concentrations, as well as the overall pulmonary gas exchange during the exercise were not affected by the pyruvate ingestion. In conclusion, the sodium pyruvate ingestion one hour before workout modified the blood acid-base status and the lactate production during the exercise.

Single pyruvate intake induces blood alkalization and modification of resting metabolism in humans

“…In the previous study, 1 h after single oral NaP ingestion, blood pH, HCO 3 À , and BE achieved 7.415, 27.15, and 2.18 mmol/L, respectively [18]. Similar values were observed in the present study, but more time was necessary to reach the peak.…”

“…The buffering character of pyruvate is consistent with the earlier results [18]. In the previous study, 1 h after single oral NaP ingestion, blood pH, HCO 3 À , and BE achieved 7.415, 27.15, and 2.18 mmol/L, respectively [18].…”

Pyruvate anaplerosis is a mechanism of resistance to pharmacological glutaminase inhibition in triple-receptor negative breast cancer

“…We show that extracellular pyruvate, at physiological concentrations of 20-100 μM, can significantly impair CB-839 potency in vitro by acting as an anaplerotic substrate. Normal blood pyruvate concentration is reported in the range of 30-150 μM [26][27][28]. Furthermore, we demonstrate that paracrine secretion of de novo produced pyruvate into the extracellular environment can act as a source of pyruvate and this process can be antagonised using a monocarboxylate transporter 1 (MCT1) inhibitor.…”

Adaptive Changes After 2 Weeks of 10-s Sprint Interval Training With Various Recovery Times

“…Due to the small number of subjects, the effect size (η 2 ) has been calculated. The values of η 2 has been interpreted as follows: 0.1 a small effect, 0.3 a medium effect and 0.5 a large effect, as previously (Olek et al, 2014 ). All data are expressed as mean ± SEM (standard error of mean).…”