Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection

Zhu, Wenjuan; Wang, Qi; Ma, Hongmin; Lv, Xiaohui; Wu, Dan; Sun, Xu; Du, Bin; Wei, Qin

doi:10.1038/srep24599

Cited by 14 publications

(7 citation statements)

References 40 publications

(44 reference statements)

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“…Subsequently, the FT-IR spectrum was recorded as shown in Figure 1c, in which the typical peaks of 1552, 1317, 1187, and 917 cm −1 prove the successful preparation of PPy according to the literature. 42,43 These data proved that PPy NPs with excellent monodispersity in aqueous solution and with a diameter of about 70 nm were successfully prepared. This should be due to the fact that the complexation of Fe 3+ with the water-soluble PVA chain resulted in a slow polymerization of PPy, and during the polymerization, the water-soluble PVA provided sufficient steric stability for the nuclei of PPy NPs.…”

Section: ■ Results and Discussionmentioning

confidence: 66%

Cell-Laden Electroconductive Hydrogel Simulating Nerve Matrix To Deliver Electrical Cues and Promote Neurogenesis

Liu

Chen

et al. 2019

ACS Appl. Mater. Interfaces

101

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Natural nerve tissue is composed of nerve bundles with multiple aligned assembles, and matrix electroconductivity is beneficial to the transmission of intercellular electrical signals, or effectively deliver external electrical cues to cells. Herein, aiming at the biomimetic design of the extracellular matrix for neurons, we first synthesized electroconductive polypyrrole (PPy) nanoparticles with modified hydrophilicity to improve their uniformity in collagen hydrogel. Next, cell-laden collagen−PPy hybrid hydrogel microfibers with highly oriented microstructures were fabricated via a microfluidic chip. The hydrogel microfibers formed a biomimetic three-dimensional microenvironment for neurons, resulting from the native cell adhesion domains, oriented fibrous structures, and conductivity. The oriented fibrous microstructures enhanced neuron-like cells aligning with fibers' axon; the matrix conductivity improved cell extension and upregulated neuralrelated gene expression; moreover, external electrical stimulation further promoted the neuronal functional expression. This mechanism was attributed to the electroconductive matrix and its delivered electrical stimulation to cells synergistically upregulated the expression of an L-type voltage-gated calcium channel, resulting in an increase in the intracellular calcium level, which in turn promoted neurogenesis. This approach has potential in constructing the biomimetic microenvironment for neurogenesis.

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Section: ■ Results and Discussionmentioning

confidence: 66%

Cell-Laden Electroconductive Hydrogel Simulating Nerve Matrix To Deliver Electrical Cues and Promote Neurogenesis

Liu

Chen

et al. 2019

ACS Appl. Mater. Interfaces

101

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“…The FTIR spectrum of PPy shows the typical peaks that can be attributed to the C−H in-plane deformation vibration at 1039 cm −1 and the ring-tensile mode of the Py ring at 1547 cm −1 . 32,33 These typical peaks from PPy are clearly shown in the FTIR spectrum of AFCP (1046 and 1555 cm −1 ). This further suggested that the PPy NPs have been successfully coated on the electrospun fibers.…”

Section: Fabrication Of Aligned Pcl-col-ppy Scaffolds Bymentioning

confidence: 94%

Combining Electrospinning and Electrospraying to Prepare a Biomimetic Neural Scaffold with Synergistic Cues of Topography and Electrotransduction

Tang

Chen

et al. 2020

ACS Appl. Bio Mater.

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The nerve tissue consists of aligned fibrous nerve bundles, in which neurons communicate and transmit information through electrical signals. Hence, biocompatibility, oriented fibrous structure, and electrical conductivity are key factors for the biomimetic design of nerve scaffolds. Herein, we built a technical platform to combine electrospinning and electrospraying for preparing a biomimetic scaffold with conductivity and aligned fibrous structure. The highly aligned polycaprolactone (PCL) microfibrous scaffolds with co-sprayed collagen and conductive polypyrrole nanoparticles (PPy NPs) showed good bioactivity, supplying a platform for exploring the effects of topographical guidance, fiber conductivity, and its mediated external electrical signals on neurogenesis. The results revealed that collagen-coated highly aligned PCL microfibrous scaffold induced PC12 cells oriented and elongated along the direction of fibers. In addition, the improved conductivity of PPy-coated aligned fibers and its mediated external electrical stimulation collectively contributed to the functional expression, including elongation, gene expression, and protein expression, of PC12 cells. We further demonstrated the potential mechanism where the fiber conductivity and its mediated external electrical signals resulted in the upregulation of voltage-gated calcium channel, leading to the influx of Ca2+, thereby activating intracellular signaling cascades, ultimately enhancing neurogenesis. This approach provides a strategy to design aligned fibrillary scaffolds with bioactive adhesion domains and electroconductivity for neural regeneration.

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“…Zhu et al constructed a novel ECL immune-based biosensor using anti-CEA–luminol–AuNP@PPy ( Figure 11 A) [ 144 ]. The PPy nanostructure made it possible to enhance the conductivity of the biosensor and provided a high specific surface area for the combination with the AuNPs, thus enabling the attachment of abundant amounts of the ECL reagent (luminol) and promoting the immobilization of antibodies.…”

Section: Ppy-based Biosensors For Crc Biomarker Detectionmentioning

confidence: 99%

Application of Polypyrrole-Based Electrochemical Biosensor for the Early Diagnosis of Colorectal Cancer

et al. 2023

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Although colorectal cancer (CRC) is easy to treat surgically and can be combined with postoperative chemotherapy, its five-year survival rate is still not optimistic. Therefore, developing sensitive, efficient, and compliant detection technology is essential to diagnose CRC at an early stage, providing more opportunities for effective treatment and intervention. Currently, the widely used clinical CRC detection methods include endoscopy, stool examination, imaging modalities, and tumor biomarker detection; among them, blood biomarkers, a noninvasive strategy for CRC screening, have shown significant potential for early diagnosis, prediction, prognosis, and staging of cancer. As shown by recent studies, electrochemical biosensors have attracted extensive attention for the detection of blood biomarkers because of their advantages of being cost-effective and having sound sensitivity, good versatility, high selectivity, and a fast response. Among these, nano-conductive polymer materials, especially the conductive polymer polypyrrole (PPy), have been broadly applied to improve sensing performance due to their excellent electrical properties and the flexibility of their surface properties, as well as their easy preparation and functionalization and good biocompatibility. This review mainly discusses the characteristics of PPy-based biosensors, their synthetic methods, and their application for the detection of CRC biomarkers. Finally, the opportunities and challenges related to the use of PPy-based sensors for diagnosing CRC are also discussed.

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Single-step cycle pulse operation of the label-free electrochemiluminescence immunosensor based on branched polypyrrole for carcinoembryonic antigen detection

Cited by 14 publications

References 40 publications

Cell-Laden Electroconductive Hydrogel Simulating Nerve Matrix To Deliver Electrical Cues and Promote Neurogenesis

Cell-Laden Electroconductive Hydrogel Simulating Nerve Matrix To Deliver Electrical Cues and Promote Neurogenesis

Combining Electrospinning and Electrospraying to Prepare a Biomimetic Neural Scaffold with Synergistic Cues of Topography and Electrotransduction

Application of Polypyrrole-Based Electrochemical Biosensor for the Early Diagnosis of Colorectal Cancer

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