Single step, rapid identification of pathogenic microorganisms in a culture bottle

Abstract

Efforts to treat bloodstream infections, which have a relatively high mortality rate, are delayed by the lengthy multi-step process required to identify the causative bacteria. Due to this delay, broad spectrum antibiotics are prescribed on a presumptive basis, leading to the rise of antibiotic resistant microorganisms. Here, as proof of principle, we describe a colourimetric sensor that rapidly identifies opportunistic pathogenic bacteria in a single step in TSB media. The device is composed of a reaction chamber and an array of chemoresponsive dyes deposited on a substrate in a prearranged pattern. This single step, disposable, automated system can detect and identify of eight strains of bacteria, starting with clinically relevant concentrations bacteria in twenty four hours in TSB media. Thus, this technology may be used to streamline the current blood culture process by combining detection and identification in a single step.

Detection of bacterial metabolites for the discrimination of bacteria utilizing gold nanoparticle chemiresistor sensors

“…The results (Fig. 5) demonstrated clear discrimination of the bacteria after 24 h. A similar result was reported by Chu et al [9] who used a colourimetric sensor to discriminate different strains of bacteria integrated into a blood culture bottle. Like this application, the AuNP chemiresistor sensor array described here has the potential to be used for real time monitoring within culture bottles over a 24 h period.…”

“…As the results from the blank control did not indicate such a change, it can be assumed that the analytes have derived from E. coli growth. This sort of transient trend has been reported by other types of sensors utilized for monitoring bacterial growth [9,30].…”

“…As yet, no single method has met the key ideal requirements of being rapid, specific, inexpensive and simple to use. To address this challenge, sensor technologies are being widely investigated as a means to rapidly detect bacteria [4][5][6][7][8][9] including label free detection [10].…”

Analysis of pathogenic bacteria using exogenous volatile organic compound metabolites and optical sensor detection

“…For example, a disposable colorimetric sensing array to identify 10 strains of bacteria has been developed. 6 Dyes incorporated into the sensor changed colour upon exposure to VOCs generated by bacteria. The use of enzyme substrates as a potential tool for bacterial detection, via exogenous VOC evolution, has previously been described.…”

Toward Rapid Detection of Viable Bacteria in Whole Blood for Early Sepsis Diagnostics and Susceptibility Testing

“…Alternative promising technologies are now being developed based on different detection strategies. Chu et al ., 11 developed a colorimetric sensor for the detection of low concentrations (5–300 CFU/mL) of eight strains of bacteria using tryptic soy broth (TSB) media. Although high sensitivity is demonstrated, this method has two important drawbacks, namely, (i) the need for sample pretreatment when using real blood samples (ii) and the duration of the assay, which expands up to 24 h. 11 This long incubation time is the most limiting aspect of these bioassays for their implementation as routine techniques.…”