In view of the intelligent demand of tennis line examination, this paper performs a systematic analysis on the intelligent recognition of tennis line examination. Then, a tennis line recognition method based on machine vision is proposed. In this paper, the color region of the image recognition region is divided based on the region growth, and the rough estimation of the court boundary is realized. In order to achieve the effect of camera calibration, a fast camera calibration method which can be used for a variety of court types is proposed. On the basis of camera calibration, a tennis line examination and segmentation system based on machine vision analysis is constructed, and the experimental results are verified by design experiments. The results show that the machine vision analysis-based intelligent segmentation system of tennis line examination has high recognition accuracy and can meet the actual needs of tennis line examination.