Single-Walled Carbon Nanotubes Downregulate Stress-Responsive Genes in Human Respiratory Tract Cells

Hitoshi, Kotaro; Katoh, Miki; Suzuki, Tomoko; Ando, Yumiko; Nadai, Masayuki

doi:10.1248/bpb.35.455

Cited by 4 publications

(1 citation statement)

References 45 publications

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“…THP-1 cells were employed to represent the resident phagocytic cells that have the main function of removing pathogens, senescent cells and external particles from the lungs2930, while A549 cells represented an in vitro model for lung alveoli cells, which are not associated with inflammatory responses. A549 cells have been extensively used for assessing pulmonary cytotoxicity, including nanomaterials-induced cytotoxicity103132.…”

Section: Discussionmentioning

confidence: 99%

Citrullination as early-stage indicator of cell response to Single-Walled Carbon Nanotubes

Mohamed

Movia

Knyazev

et al. 2013

Sci Rep

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Single-walled carbon nanotubes (SWCNTs) have been widely explored as potential technologies for information systems and medical applications. The impact of SWCNTs on human health is of prime concern, if SWCNTs have a future in the manufacturing industry. This study proposes a novel, inflammation-independent paradigm of toxicity for SWCNTs, identifying the protein citrullination process as early-stage indicator of inflammatory responses of macrophages (THP-1) and of subtle phenotypic damages of lung epithelial (A549) cells following exposure to chemically-treated SWCNTs. Our results showed that, while most of the cellular responses of A549 cells exposed to SWCNTs are different to those of similarly treated THP-1 cells, the protein citrullination process is triggered in a dose- and time-dependent manner in both cell lines, with thresholds comparable between inflammatory (THP-1) and non-inflammatory (A549) cell types. The cellular mechanism proposed herein could have a high impact in predicting the current risk associated with environmental exposure to SWCNTs.

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Section: Discussionmentioning

confidence: 99%

Citrullination as early-stage indicator of cell response to Single-Walled Carbon Nanotubes

Mohamed

Movia

Knyazev

et al. 2013

Sci Rep

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Genotoxicity but not the AhR-mediated activity of PAHs is inhibited by other components of complex mixtures of ambient air pollutants

et al. 2014

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In this study, we compared the genotoxicity and aryl hydrocarbon receptor (AhR)-dependent transcriptional changes of selected target genes in human lung epithelial A549 cells incubated for 24 h, either with extractable organic matter (EOMs) from airborne particles <2.5 μm (PM2.5) collected at four localities from heavily polluted areas of the Czech Republic or two representative toxic polycyclic aromatic hydrocarbons (PAHs) present in EOMs, benzo[a]pyrene (B[a]P) and benzo[k]fluoranthene (B[k]F). Genotoxic effects were determined using DNA adduct analysis or analysis of expression of selected AhR-related genes involved in bioactivation of PAHs (CYP1A1, CYP1B1) and transcriptional repression (TIPARP). Sampled localities differing in the extent and source of air pollution did not exhibit substantially different genotoxicity. DNA adduct levels induced by three subtoxic EOM concentrations were relatively low (1-5 adducts/10(8) nucleotides), compared to levels induced by similar concentrations of B[a]P, while B[k]F gave very low DNA adduct levels. Here, we compared genotoxicity and gene deregulation induced by complex mixtures containing PAHs with the effects of the comparable concentrations of individual PAHs. Our results suggested inhibition of formation of B[a]P-induced DNA adducts compared to individual B[a]P, probably attributable to competitive inhibition by other non-genotoxic EOM components. In contrast, induction of AhR target genes appeared not to be antagonized by the components of complex mixtures, as induction of CYP1A1, CYP1B1 and TIPARP transcripts reached maximum levels induced by PAHs.

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X-ray Absorption Improvement of Single Wall Carbon Nanotube through Gadolinium Encapsulation

Alimin

Narsito

Kartini

et al. 2016

IOP Conf. Ser.: Mater. Sci. Eng.

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Single-Walled Carbon Nanotubes Downregulate Stress-Responsive Genes in Human Respiratory Tract Cells

Cited by 4 publications

References 45 publications

Citrullination as early-stage indicator of cell response to Single-Walled Carbon Nanotubes

Citrullination as early-stage indicator of cell response to Single-Walled Carbon Nanotubes

Genotoxicity but not the AhR-mediated activity of PAHs is inhibited by other components of complex mixtures of ambient air pollutants

X-ray Absorption Improvement of Single Wall Carbon Nanotube through Gadolinium Encapsulation

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