Single-walled carbon nanotubes (SWCNTs) induce vasodilation in isolated rat aortic rings

Gutiérrez-Hernández, José Manuel; Ramirez-Lee, Manuel Alejandro; Rosas-Hernández, Héctor; Salazar-García, Samuel; Maldonado-Ortega, Daniel Alberto; González, Francisco

doi:10.1016/j.tiv.2015.02.001

Cited by 9 publications

(2 citation statements)

References 31 publications

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“…The cytotoxicity assay indicated that all types of MWCNTs significantly decreased cellular viability to a similar extent (Figure 3). Previous studies showed that the cytotoxicity of MWCNTs17 or single-walled carbon nanotubes23 to endothelial cells could be increased once they were carboxylated. Orecna et al17 further suggested that the cytotoxicity of carboxylated MWCNTs to human endothelial cells was due to the blockade of autophagic flux.…”

Section: Discussionmentioning

confidence: 99%

<p>Multi-Walled Carbon Nanotubes (MWCNTs) Activate Apoptotic Pathway Through ER Stress: Does Surface Chemistry Matter?</p>

Sun¹,

Gong²,

Cao³

2019

IJN

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PurposePhysicochemical properties play a crucial role in determining the toxicity of multi-walled carbon nanotubes (MWCNTs). Recently we found that MWCNTs with longer length and smaller diameters could induce toxicity to human umbilical vein endothelial cells (HUVECs) through the activation of endoplasmic reticulum (ER) stress. In this study, we further investigated the possible contribution of hydroxylation and carboxylation to the cytotoxicity of MWCNTs.MethodsThe HUVECs were exposed to pristine (code XFM19), hydroxylated (code XFM20; content of hydroxyl groups 1.76 wt%) and carboxylated (code XFM21; content of carboxyl groups 1.23 wt%) MWCNTs, respectively. Then, the internalization, cytotoxicity, oxidative stress and activation of apoptosis-ER stress pathway were measured.ResultsIn consequence, all types of MWCNTs could be internalized into the HUVECs, and the cellular viability was significantly reduced to a similar level. Moreover, the MWCNTs increased intracellular reactive oxygen species (ROS) and decreased glutathione (GSH) to similar levels, indicating their capacity of inducing oxidative stress. The Western blot results showed that all types of MWCNTs reduced BCL-2 and increased caspase-3, caspase-8, cleaved caspase-3 and cleaved caspase-8. The expression of ER stress gene DNA damage-inducible transcript 3 (DDIT3) and protein level of chop were only significantly induced by XFM20 and XFM21, whereas protein level of p-chop was promoted by XFM19 and XFM21. In addition, the pro-survival gene XBP-1s was significantly down-regulated by all types of MWCNTs.ConclusionThese results suggested that MWCNTs could induce cytotoxicity to HUVECs via the induction of oxidative stress and apoptosis-ER stress, whereas a low degree of hydroxylation or carboxylation did not affect the toxicity of MWCNTs to HUVECs.

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Section: Discussionmentioning

confidence: 99%

<p>Multi-Walled Carbon Nanotubes (MWCNTs) Activate Apoptotic Pathway Through ER Stress: Does Surface Chemistry Matter?</p>

Sun¹,

Gong²,

Cao³

2019

IJN

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“…In previous in vivo and in vitro studies, carbon-based nanosized allotropes (mostly single-walled carbon nanotubes, multi-walled carbon nanotubes and CB, diamond and graphite NPs, C 60 ) have been widely described to cause the inhibition of angiogenesis and vasorelaxation, enhance monocyte adhesion even atherosclerosis and other adverse effects on the cardiovascular system (Cao, Jacobsen, et al, 2014;Cao, Roursgaard, Danielsen, Moller, & Loft, 2014;Gutierrez-Hernandez et al, 2015;Thompson et al, 2014;Wierzbicki et al, 2013). Lately, there was mounting epidemiological evidence of a link between long-term BC exposure and human cardiovascular diseases.…”

Section: Discussionmentioning

confidence: 99%

Prolonged exposure to carbon nanoparticles induced methylome remodeling and gene expression in zebrafish heart

Zhou

Tian²,

et al. 2018

J of Applied Toxicology

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Growing black carbon (BC) emission has become one of the major urgent environmental issues facing human beings. Usually, BC or BC‐containing carbon nanoparticles (CNPs) were recognized as non‐directly toxic components of atmospheric particulate matter. However, epidemiology studies have provided much evidence of the associations of exposure of particulate‐containing carbon particles with cardiovascular diseases. There are still no related studies to support the epidemiological conclusions. Hence, in this article we exposed adult zebrafish to CNPs for 60 days, and then explored the heart location and potential adverse effects on cardiac tissues of these nanosized carbon particles. Our results first showed direct visualization of cardiac endothelial uptake and heart deposition of CNPs in zebrafish. In addition, CNPs caused significant ultrastructural alterations in myocardial tissue and induced the expression of inflammatory cytokines in a dose‐dependent manner, resulting in sub‐endocardial inflammation and cell apoptosis. Moreover, our data demonstrated the perturbations caused by CNPs on DNA methylation, suggesting that DNA methylome remodeling might play a critical role in CNP‐induced cardiotoxicity in zebrafish heart. Therefore, this study not only proved a laboratory link between CNP exposure and cardiotoxicity in vivo, but also indicated a possible toxicity mechanism involved.

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Systemic Administrations of Water-Dispersible Single-Walled Carbon Nanotubes: Activation of NOS in Spontaneously Hypertensive Rats

et al. 2020

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Single-walled carbon nanotubes (SWCNTs) induce vasodilation in isolated rat aortic rings

Cited by 9 publications

References 31 publications

<p>Multi-Walled Carbon Nanotubes (MWCNTs) Activate Apoptotic Pathway Through ER Stress: Does Surface Chemistry Matter?</p>

<p>Multi-Walled Carbon Nanotubes (MWCNTs) Activate Apoptotic Pathway Through ER Stress: Does Surface Chemistry Matter?</p>

Prolonged exposure to carbon nanoparticles induced methylome remodeling and gene expression in zebrafish heart

Systemic Administrations of Water-Dispersible Single-Walled Carbon Nanotubes: Activation of NOS in Spontaneously Hypertensive Rats

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