Singlet Oxygen Inhibits the Repair of Photosystem II by Suppressing the Translation Elongation of the D1 Protein in <i>Synechocystis</i> sp. PCC 6803

Nishiyama, Yoshitaka; Allakhverdiev, Suleyman I.; Yamamoto, Hiroshi; Hayashi, Hidenori; Murata, Norio

doi:10.1021/bi036178q

Cited by 296 publications

(272 citation statements)

References 42 publications

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“…Mutants without zeaxanthin or echinenone and especially the double mutant defective of both zeaxanthin and echinenone are sensitive to photooxidative damage (Schäfer et al, 2005). Salt stress has been suggested to induce ROS production, and both photosynthesis and translation are known to be ROS sensitive (Nishiyama et al, 2004(Nishiyama et al, , 2006Kojima et al, 2007). Enhanced accumulation of carotenoids in salt stress might actually be important for reactivation of photosynthesis and translation during salt acclimation.…”

Section: Sigb Factor Regulates the Carotenoid Content Of The Cellsmentioning

confidence: 99%

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

et al. 2011

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Changing of principal s factor in RNA polymerase holoenzyme to a group 2 s factor redirects transcription when cyanobacteria acclimate to suboptimal environmental conditions. The group 2 sigma factor SigB was found to be important for the growth of the cyanobacterium Synechocystis sp. PCC 6803 in high-salt (0.7 M NaCl) stress but not in mild heat stress at 43°C although the expression of the sigB gene was similarly highly, but only transiently up-regulated at both conditions. The SigB factor was found to regulate many salt acclimation processes. The amount of glucosylglycerol-phosphate synthase, a key enzyme in the production of the compatible solute glucosylglycerol, was lower in the inactivation strain DsigB than in the control strain. Addition of the compatible solute trehalose almost completely restored the growth of the DsigB strain at 0.7 M NaCl. High-salt conditions lowered the chlorophyll and phycobilin contents of the cells while protective carotenoid pigments, especially zeaxanthin and myxoxanthophyll, were up-regulated in the control strain. These carotenoids were up-regulated in the DsigCDE strain (SigB is the only functional group 2 s factor) and down-regulated in the DsigB strain under standard conditions. In addition, the HspA heat shock protein was less abundant and more abundant in the DsigB and DsigCDE strains, respectively, than in the control strain in high-salt conditions. Some cellular responses are common to heat and salt stresses, but pretreatment with mild heat did not protect cells against salt shock although protection against heat shock was evident.

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Section: Sigb Factor Regulates the Carotenoid Content Of The Cellsmentioning

confidence: 99%

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

et al. 2011

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“…3), indicating that the acceleration of photodamage upon the impairment of CEF is attributable to neither the impairment of qE nor the production of hydrogen peroxide. Furthermore, both interruption of the Calvin cycle that causes overreduction of photosynthetic electron transport (Hakala et al, 2005;Murata, 2005, 2006) and the exogenous supply of singlet oxygen (Nishiyama et al, 2004) have been demonstrated to have no effect on the process of photodamage. However, nigericin accelerated photoinhibition in both the presence and absence of chloramphenicol (Fig.…”

Section: Cef Reduces Direct Photodamage To Psiimentioning

confidence: 99%

“…In cyanobacteria, exogenous supply of singlet oxygen and hydrogen peroxide has been demonstrated to inhibit the repair of photodamaged PSII with no effect on the process of photodamage to PSII (Nishiyama et al, 2001(Nishiyama et al, , 2004. This inhibition of the repair occurs through inhibition of the de novo synthesis of PSII proteins, primarily the D1 protein, at the step of translation (Nishiyama et al, 2004Kojima et al, 2007). Inhibition of the synthesis of the D1 protein upon impairment of qE and CEF, therefore, might be attributable to the production of reactive oxygen species (Fig.…”

Section: Cef Reduces Inhibition Of Protein Synthesis-dependent Repairmentioning

confidence: 99%

How Does Cyclic Electron Flow Alleviate Photoinhibition in Arabidopsis?

et al. 2008

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Cyclic electron flow (CEF) around photosystem I has a role in avoiding photoinhibition of photosystem II (PSII), which occurs under conditions in which the rate of photodamage to PSII exceeds the rate of its repair. However, the molecular mechanism underlying how CEF contributes to photoprotection is not yet well understood. We examined the effect of impairment of CEF and thermal energy dissipation (qE) on photoinhibition using CEF (pgr5) and qE (npq1 and npq4) mutants of Arabidopsis (Arabidopsis thaliana) exposed to strong light. Impairment of CEF by mutation of pgr5 suppressed qE and accelerated photoinhibition. We found that impairment of qE, by mutations of pgr5, npq1, and npq4, caused inhibition of the repair of photodamaged PSII at the step of the de novo synthesis of the D1 protein. In the presence of the chloroplast protein synthesis inhibitor chloramphenicol, impairment of CEF, but not impairment of qE, accelerated photoinhibition, and a similar effect was obtained when leaves were infiltrated with the protonophore nigericin. These results suggest that CEF-dependent generation of DpH across the thylakoid membrane helps to alleviate photoinhibition by at least two different photoprotection mechanisms: one is linked to qE generation and prevents the inhibition of the repair of photodamaged PSII at the step of protein synthesis, and the other is independent of qE and suppresses photodamage to PSII.

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“…Since the reduction state of Q A linearly increases with irradiance, the probability of photodamage increases under strong light (Melis 1999). While light damages PSII directly, oxidative stress during photosynthesis has been demonstrated to suppress the de novo synthesis of proteins, in particular, the D1 protein, which is required for the repair of PSII (Nishiyama et al 2004. Photodamage or chronic photoinhibition mainly occurs in seaweeds growing in the lower sublittoral zone when exposed to high irradiances (Hanelt 1998).…”

Section: Introductionmentioning

confidence: 99%

Exposure to ultraviolet radiation delays photosynthetic recovery in Arctic kelp zoospores

Roleda

Hanelt²,

Wiencke

2006

Photosynth Res

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Seasonal reproduction in some Arctic Laminariales coincides with increased UV-B radiation due to stratospheric ozone depletion and relatively high water temperatures during polar spring. To find out the capacity to cope with different spectral irradiance, the kinetics of photosynthetic recovery was investigated in zoospores of four Arctic species of the order Laminariales, the kelps Saccorhiza dermatodea, Alaria esculenta, Laminaria digitata, and Laminaria saccharina. The physiology of light harvesting, changes in photosynthetic efficiency and kinetics of photosynthetic recovery were measured by in vivo fluorescence changes of Photosystem II (PSII). Saturation irradiance of freshly released spores showed minimal I k values (photon fluence rate where initial slope intersects horizontal asymptote of the curve) values ranging from 13 to 18 lmol photons m )2 s )1 among species collected at different depths, confirming that spores are low-light adapted. Exposure to different radiation spectra consisting of photosynthetically active radiation (PAR; 400-700 nm), PAR+UV-A radiation (UV-A; 320-400 nm), and PAR+ UV-A+UV-B radiation (UV-B; 280-320 nm) showed that the cumulative effects of increasing PAR fluence and the additional effect of UV-A and UV-B radiations on photoinhibition of photosynthesis are species specific. After long exposures, Laminaria saccharina was more sensitive to the different light treatments than the other three species investigated. Kinetics of recovery in zoospores showed a fast phase in S. dermatodea, which indicates a reduction of the photoprotective process while a slow phase in L. saccharina indicates recovery from severe photodamage. This first attempt to study photoinhibition and kinetics of recovery in zoospores showed that zoospores are the stage in the life history of seaweeds most susceptible to light stress and that ultraviolet radiation (UVR) effectively delays photosynthetic recovery. The viability of spores is important on the recruitment of the gametophytic and sporophytic life stages. The impact of UVR on the zoospores is related to the vertical depth distribution of the large sporophytes in the field.

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Singlet Oxygen Inhibits the Repair of Photosystem II by Suppressing the Translation Elongation of the D1 Protein in Synechocystis sp. PCC 6803

Cited by 296 publications

References 42 publications

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

How Does Cyclic Electron Flow Alleviate Photoinhibition in Arabidopsis?

Exposure to ultraviolet radiation delays photosynthetic recovery in Arctic kelp zoospores

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