Key words Matrix differential operator, essential spectrum, Hamiltonian system, deficiency index MSC (2010) Primary: 34L05, Secondary: 34B20, 34L40The present paper is concerned with the essential spectrum of the singular matrix differential operator of mixed orderIt is shown that if the coefficients are locally integrable functions and T is in the limit circle case, then the essential spectrum of T is given by the essential range of. The main idea is to transform the original spectral problem into a spectral problem of a singular Hamiltonian differential operator so that the classical Titchmarsh-Weyl theory can apply. The approach used here can be applied to many other cases.