Sinomenine inhibits primary CD4⁺ T‐cell proliferation via apoptosis

Abstract

Sinomenine is an active component isolated from Sinomenium acutum and is widely used as an immunosuppressive drug for treating autoimmune diseases. CD4(+) T-cell population plays a key role in adaptive immune response and is related to some autoimmune diseases. In this study, we investigated the possible immunosuppressive effect of sinomenine on CD4(+) T cells and its underlying mechanism. Our data demonstrated that sinomenine remarkably suppressed the proliferation of CD4(+) T cells, blocked the cell cycle progression from G0/G1 phase to S plusG2/M phases. Finally, the immunosuppressive activity elicited by sinomenine in CD4(+) primary lymphocytes was found to be largely accounted for by caspase 3-dependent cells apoptosis. Sinomenine did not significantly alter the expression of bcl-2 in activated CD4(+) primary T cells, suggesting that bcl-2 might not be involved in sinomenine-induced T cells apoptosis. In sum, this study proposes a novel mechanism for the immunosuppressive function of sinomenine on primary mouse CD4(+) T cells.

Suppressive Effect of Sinomenine Combined with 5-Fluorouracil on Colon Carcinoma Cell Growth

“…The topical application of SIN in the treatment of rheumatoid arthritis has potential advantages (Zheng et al, 2007). It has previously been demonstrated that SIN exhibited a wide range of pharmacological actions including anti-inflammatory, anti-rheumatic, immunosuppressive, analgesic and antiarrhythmic effects (Shu et al, 2007;Teng et al, 2012;Song et al, 2013). And there are many other researches indicate that SIN has many pharmacological characters such as inducing apoptosis and anti-angiogenesis effect what has been treated as means of treating colon carcinoma (Kok et al, 2005;Ju et al, 2010;Chen et al, 2011).…”

“…Extracted from the Chinese medicinal plant, Sinomenium acutum, SIN has been utilized to treat rheumatoid arthritis (RA) in China for over 2000 years. Previous reports have demonstrated a wide range of pharmacological actions including anti-inflammatory, anti-rheumatic, immunosuppressive, analgesic and antiarrhythmic effects (Shu et al, 2007;Teng et al, 2012;Song et al, 2013). But its potential value of anti-tumor by inhibiting proliferation and induced apoptosis was recognized in recent years (He et al, 2005;Li et al, 2008).…”

“…Many reports show that SIN has the potent of inhibiting proliferation and inducing apoptosis on chondrocytes (Ju et al, 2010), CD4+ T cells (Shu et al, 2007), monocytes (Jiang et al, 2007) and tumor cells (Jiang et al, 2010). But the specific mechanism about how SIN inducing apoptosis has not been researched clearly.…”

Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation

“…Therefore, we hypothesized that SIN could also inhibit bone resorption and the survival of mature osteoclasts by inducing osteoclast apoptosis. Recently, SIN has been shown to modulate caspase activation to induce apoptosis [9,12,13] . However, there is currently no report about the effect of SIN on the apoptosis of osteoclasts.…”

“…We found that SIN can significantly inhibit www.nature.com/aps He LG et al Acta Pharmacologica Sinica npg osteoclastogenesis and resorptive activity in vitro and in vivo (data not shown). Notably, SIN is known to induce apoptosis in various types of cells [9][10][11][12][13][14] . Therefore, we hypothesized that SIN could also inhibit bone resorption and the survival of mature osteoclasts by inducing osteoclast apoptosis.…”