Sister Chromatid Exchange in Garlic (<i>Allium Sativum</i>L.) Meristem Root-Tip Cells

Doležel, Jaroslav; Číhalíková, Jarmila; Novák, František

doi:10.1080/00087114.1986.10797766

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Treatment means for cells which had been exposed to two cell cycles were at least halved when compared to unifilarly substituted cells at the same concentration. These results are in agreement with Dolezel et al (1986). No trend toward a decrease in the mitotic index was noted in plants substituted at increasing concentrations of BrdU.…”

Section: Factors Affecting the Mitotic Indexsupporting

confidence: 82%

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Gerster

Grant

1989

CYTOLOGIA

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Section: Factors Affecting the Mitotic Indexsupporting

confidence: 82%

A cytogenetic study of factors affecting sister chromatid differentiation in Vicia faba and Hordeum vulgare.

Gerster

Grant

1989

CYTOLOGIA

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“…SCE studies with chromosomes of Vicia (G eard and P eacock 1969); hamster (W olff and P erry 1974), Allium (S chvarztman and C ortes 1977; C ortés et al 1983; D olezel et al 1986), state that SCE were distributed accordingly to a Poisson distribution. In the present study the statistical results are consistent with these authors.…”

Section: Discussionmentioning

confidence: 99%

Statistical and image analysis of sister chromatid exchange in maize

2005

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The present study reports the use of the fluorescence plus Giemsa (FPG) technique, image analysis and statistical methods to assess the sister chromatid exchanges (SCEs) frequency in maize. Roots derived from germinated maize seeds were treated with BrdU solution and fixed. The slides were prepared by enzymatic cellular dissociation, air-drying technique, stained with Hoechst 33258 fluorochrome, and incubated in salt solution. The chromosomes were irradiated with ultraviolet light and stained with Giemsa solution. The FPG technique associated with digital analysis system was used to measure the length of 597 BrdU-incorporated maize chromosomes and to identify 0.5243 SCE per chromosome. A range from 0 to 4 SCE events were classified and the chi-square test (chi2=1.586, P=0.662) showed a good fit to the hypothesis that the SCEs are independent and random events that follow Poisson distribution. The SCE frequencies in long and short chromosome arms corresponded to a mean value of 0.876 SCE microm(-1). Considering that the maize line used in this study contains 5.78 picogram (pg) DNA (2C value) in interphasic G0/G1 nuclei or 11.56 pg DNA (4C value) in metaphase, and that the DNA mean value corresponds to 0.578 pg/metaphasic chromosome, the analysis suggests an occurrence of approximately 0.9 SCE/pg DNA.

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