Sister chromatid exchanges in barley

Abstract

A mean frequency of 20.6 sister chromatid exchanges (SCEs) per cell has been observed in a reconstructed karyotype of Hordeum vulgare by application of the FPG technique after unifilar incorporation of BrdU into chromosomes. The involvement in SCEs of the 48 segments into which the chromosome set had been subdivided was, with a single deviation, length proportional and independent of the segment's heterochromatin content. Asymmetric bands, indicative of an uneven distribution of adenine and thymidine between the DNA strands in adenine (A)-thymidine (T) rich chromosome regions, could not be detected after incubation of the cells in BrdU for one cycle of DNA replication.

Sister Chromatid Exchange in Garlic (Allium SativumL.) Meristem Root-Tip Cells

“…The optimal concentration was 10 times lower. Similarly ScHUBERT et al (1980) applied 2.5 X 10-5 M Thd for Hordeum vulgare. In contrast to ScHVARTZMAN and CoRTES (1977) we recorded that even the second interphase following the treated one was slightly prolonged.…”

“…Vicia faba (KIHLMAN and KRONBORG 1975), Allium cepa (SCHVARTZMAN and CoRTES 1977), Secale cereale (FRIEBE 1978), Zea mays (CHou and WEBER 1980), Hordeum vulgare (SCHUBERT et at. 1980), Zebrina pendula (EvANS and FILION 1980), Allium· fistulosum (SuzuKI et at.…”

Somaclonal variation — a novel source of variability from cell cultures for plant improvement

“…The estimates of frequency of sister chromatid exchange are very high (e.g. 20.6 per barley cell per division) (Schubert et al 1980). Asymmetric exchanges have been observed in Vicia faba and were thought to occur in AT-rich segments (Schubert and Rieger 1979).…”

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

“…No mutagen-induced SCEs were analyzed previously, which is crucial to test the sensitivity of a new method by using an experimental design to study the response to physical and chemical agents. The SCE test has previously been used in barley using BrdU-substituted chromatids (Schubert et al, 1980;Andronic et al, 2010). Although the results of our studies confirmed the formation of SCEs under control conditions, a direct comparison with previous data is difficult due to differences in the experimental conditions, such as the BrdU concentrations and the time of its incorporation, as well as the different barley varieties and lines that were used for the studies.…”