Site of Intracellular Antigen Production by Myxoviruses.

Traver, Myrna; Northrop, Robert L.; Walker, Duard L.

doi:10.3181/00379727-104-25803

Cited by 50 publications

(17 citation statements)

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“…A comparison was made with conjunctiva cells infected with the cytopathogenic line of mumps virus. The distribution of the antigen of the latter virus during the full cycle of multiplication has been described previously (9). The major point to be emphasized here is that in the cytopathogenic, acute infection the antigen was widely dispersed throughout the cytoplasm, first in fine granules, and later in dozens of granules and larger clumps (Fig.…”

Section: Relationship Between Cell Number and Infectious Virus Unitssupporting

confidence: 61%

A Carrier State of Mumps Virus in Human Conjunctiva Cells

Walker

Hinze

1962

The Journal of Experimental Medicine

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P~ 94(Received for publication, July 13, 1962.) Viral carrier states in cell cultures have been described by several investigators using a variety of virus-cell systems (reviewed in reference 1). Because of techniques applicable to cell cultures, such carrier states would seem to offer particularly good opportunities to gain understanding of interactions of virus and cell that may be important to those naturally occurring infections in which viruses invade and remain in hosts for relatively long periods without causing apparent harm. From the studies already reported, however, it is evident that there is considerable variation in the virus-cell relationships found in carrier systems in vitro. This is to be expected since, at least superficially, the examples of persistent, inapparent viral infections found in man and animals present a diversity of characteristics. Those carrier cultures so far described have, however, usually had several things in common in that they often have utilized cells relatively resistant to the virus; many have required antibody in the medium or have appeared to depend upon some form of interference for protection of the cells; many show evidence for infection of only a small proportion of the cells in the culture. In this and the following paper we shall describe a virus carrier system that is unusual in its combination of features that include (a) lack of any requirement for antibody, (b) indication that there is not a selection of resistant cells, (c) alteration in the cytopathogenicity of the persisting virus, (d) infection of almost all of the cells of the culture, and (e) indication that infected cells are capable of repeated division. Materials and MethodsViruses.--The Dunai strain of mumps virus was isolated from a patient with parotitis (2) by inoculation of throat washings into the amniotie sac of the chick embryo. After the initial amniofic passage, it was cultivated in human conjunctiva cells.

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Section: Relationship Between Cell Number and Infectious Virus Unitssupporting

confidence: 61%

A Carrier State of Mumps Virus in Human Conjunctiva Cells

Walker

Hinze

1962

The Journal of Experimental Medicine

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“…Immunofluorescence studies by Kisch, Johnson and Chanock (45) confirmed observations of the replicative cycle observed by others (4,68). In their more detailed observations, they noted specific fluorescence only in the cytoplasm throughout the replicative cycle (45).…”

Section: It Was In This Context That the Search For New Viral Agentssupporting

confidence: 67%

Isolation, characterization, and pathogenicity studies of a bovine respiratory syncytial virus

Smith

Frey

Dierks

1975

Archives of Virology

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“…Early studies with fluorescent antibodies indicated that synthesis of paramyxovirus proteins occurred in the cytoplasm of infected cells (Wheelock and Tamm, 1959;1961a;Traver et al, 1960;Cohen et a/., 1961). Although antigens, eosinophilic inclusions, and nucleocapsids have been seen in the nuclei of infected cells by a number of workers with various paramyxsoviruses, the weight of evidence obtained in many laboratories has indicated that paramyxovirus proteins are synthesized in the cytoplasm, and that such nuclear accumulations, which are usually seen late in infection, result from transport from the cytoplasm to the nucleus.…”

Section: Proteinsmentioning

confidence: 99%