Site-specific control of silica mineralization on DNA using a designed peptide

Abstract

We developed a site-specific method for precipitating inorganic compounds using organic compounds, DNA, and designed peptides with peptide nucleic acids (PNAs). Such a system for site-specific precipitation represents a powerful tool for use in nanobiochemistry and materials chemistry.

Interactions between Metal Oxides and Biomolecules: from Fundamental Understanding to Applications

“…To date, a wide range of SiNPs has been generated and their physical, chemical, and functional properties tuned either by directing silica nanoparticle formation directly or by modifying them with inorganic or organic molecules. The molecules reported to bind with silica NPs include proteins (silica BPs, antibodies or antibody fragments, enzymes, and streptavidin or avidin BPs), ,− nucleic acids (DNA or RNA), , other targeting molecules (ligands and peptide motifs), , as well as cells (bacterial or viral). − Continued research efforts by scientists have led to silica being a key material in the design of novel bionanostructures, further broadening its application from nanotechnology to biomedical and further industrial fields as shown schematically in Figure . A review of applications and latest patents of solid material BPs have been recently reported by Thota and Perry …”

“…Often studies employing combinations of techniques are particularly valuable. For example, in a study using a combination of atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering (DLS), Ozaki and co-workers developed a site-specific process for precipitating silica using silica BPs combined with peptide nucleic acids (PNAs) in which the PNA sequence could act as a binding module for a complementary DNA sequence . Here, AFM showed the formation of long chains and unique dumbbell-shaped silica nanospheres as a result of site-specific silica biomineralisation on DNA sites.…”

“…Here, AFM showed the formation of long chains and unique dumbbell-shaped silica nanospheres as a result of site-specific silica biomineralisation on DNA sites. In addition, transmission electron microscope (TEM) and dynamic light scattering (DLS) analysis revealed that the control of nanoparticles depended on the number of silica binding peptide-PNA conjugates attached to the DNA molecule . Furthermore, Thyparambil et al functionalized an AFM tip with an oligopeptide and obtained thermodynamic data arising from peptide adsorption on quartz and fused glass …”

Effect of tryptophan residues on gold mineralization by a gold reducing peptide

“…For example, biomineralization (precipitation of inorganic compounds) systems can provide various inorganic precipitates following the addition of organic compounds such as proteins and peptides. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] The reaction conditions for the production of inorganic compounds in biomineralization systems are much milder than those used in conventional methods. Therefore, such inorganic compounds formed by mineralization have a low environmental load and reduced energy consumption.…”

“…(1) Small peptides derived from the sequences of proteins isolated for mineralization or articial sequences can precipitate many types of inorganic compounds. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] (2) Peptides can be conjugated with articial amino acids and functional molecules. 19,[26][27][28] (3) Peptides are easier to design and handle than proteins.…”