Sites of integration of infectious DNA of avian reticuloendotheliosis viruses in different avian cellular DNAs

Battula, Narayana; Temin, Howard M.

doi:10.1016/0092-8674(78)90207-6

Cited by 39 publications

(30 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(iii) Viral DNA is also found integrated at multiple sites in chronically infected cells. However, consistent with our previous studies (6,7), infectious viral DNA is only present at a restricted fraction of these sites.…”

supporting

confidence: 81%

“…DNA fragments that contain viral nucleotide sequences were specifically detected by nucleic acid hybridization with iodinated viral RNA by the blotting technique of Southern (8) (ii) The majority of this integrated viral DNA is absent from chronically infected cells, a result consistent with the hypothesis that the cell death that occurs after infection of avian cells by reticuloendotheliosis virus is a consequence of these multiple integrations (6,7). (iii) Viral DNA is also found integrated at multiple sites in chronically infected cells.…”

supporting

confidence: 57%

“…Previous studies from our laboratory have shown that infectious reticuloendotheliosis virus DNA is integrated at multiple sites in acutely infected avian cells (soon after infection) and at a unique site in chronically infected avian cells (late after infection) (6,7). However, since the assay method used in these experiments detected only infectious viral DNA molecules, it is possible that noninfectious viral DNA is also present at additional sites in the host cell.…”

mentioning

confidence: 42%

“…Chicken cells were infected with SNV at a multiplicity of less than 1 plaque-forming unit per cell. Data on the growth of SNV-infected avian cells have been presented (7,9,10 …”

mentioning

confidence: 99%

See 3 more Smart Citations

Sites of integration of reticuloendotheliosis virus DNA in chicken DNA.

Temin¹

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The pattern of integration of spleen necrosis virus (SNV) DNA in DNA from a large population of SNV-infected chicken cells was studied by nucleic acid hybridization with iodinated viral RNA by the blotting technique of Southern. SNV DNA was found to be integrated at multiple sites in acutely infected chicken cells. Concomitant with the transition from acute to chronic infection, a shift in the pattern of integration was observed. The majority of integrated SNV DNA found in acutely infected cells was absent from chronically infected cells. This result is consistent with the hypothesis that the cell death that occurs after infection of avian cells with reticuloendotheliosis viruses is a consequence of the multiple integrations of the provirus. Viral DNA was also integrated at multiple sites in chronically infected cells. However, infectious viral DNA molecules in chronically infected cells migrated in a uniform manner in agarose gel electrophoresis after EcoRI digestion (which does not cut viral DNA), indicating that not all integrated SNV copies are equally infectious. Retrovirus DNA is stably integrated in host cell DNA (1, 2). However, little is known about the sites of integration and the mechanism by which viral DNA integrates into host cell DNA. Early after retrovirus infection of sensitive cells, many complete viral DNA molecules are synthesized and some are also found in the nucleus. Only a portion of these molecules become stably integrated in the host genome (2-5). The reason for the restricted number of integrated viral DNA molecules is unknown. A limited number of sites in host cell DNA that can integrate viral DNA molecules has been suggested (3).Previous studies from our laboratory have shown that infectious reticuloendotheliosis virus DNA is integrated at multiple sites in acutely infected avian cells (soon after infection) and at a unique site in chronically infected avian cells (late after infection) (6, 7). However, since the assay method used in these experiments detected only infectious viral DNA molecules, it is possible that noninfectious viral DNA is also present at additional sites in the host cell. We used nucleic acid hybridization techniques to test this possibility and to obtain more information about the pattern of integration of spleen necrosis virus (SNV) in chicken cell DNA.DNA from a large population of SNV-infected chicken cells was digested with restriction endonucleases. DNA fragments that contain viral nucleotide sequences were specifically detected (ii) The majority of this integrated viral DNA is absent from chronically infected cells, a result consistent with the hypothesis that the cell death that occurs after infection of avian cells by reticuloendotheliosis virus is a consequence of these multiple integrations (6, 7). (iii) Viral DNA is also found integrated at multiple sites in chronically infected cells. However, consistent with our previous studies (6, 7), infectious viral DNA is only present at a restricted fraction of these sites. MATERIALS AND METHODSThe ...

show abstract

supporting

confidence: 81%

supporting

confidence: 57%

mentioning

confidence: 42%

“…Chicken cells were infected with SNV at a multiplicity of less than 1 plaque-forming unit per cell. Data on the growth of SNV-infected avian cells have been presented (7,9,10 …”

mentioning

confidence: 99%

See 2 more Smart Citations

Sites of integration of reticuloendotheliosis virus DNA in chicken DNA.

Temin¹

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Our heteroduplex analysis (Fig. 2) (24,(31)(32)(33)(34)(35). For the MuSV variants used here, at least two distinct sites in the mink genome can be identified, and only direct sequence analysis will reveal local similarity in the actual integration sites.…”

Section: Resultsmentioning

confidence: 99%

Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.

Woude

Oskarsson

Enquist

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

100

View full text Add to dashboard Cite

We have identified integrated proviral DNA sequences of m1 and HT-1 isolates of Moloney sarcoma virus (MuSV) in EcoRI digests of transformed mink cell genomic DNA and have cloned these fragments in bacteriophage lambda. Both the lambda-HT1 phage recombinant, containing a 12.3-kilobase MuSV pair (kb) fragment, and the lambda-m1 phage recombinant, containing a 7.0-kb fragment, possess full copies of the sarcoma viruses along with 5' and 3' host flanking sequences. The MuSV proviral DNA sequences, 6.7 kb for HT-1 and 5.2 kb for m1, are colinear by heteroduplex microscopy with the 1.5-kb difference in size accounted for by two approximately equal to 0.8-kb deleted regions in m1. Both integrated viral genomes are terminally redundant and have integrated at the same site in the provirus but at different sites on the host chromosome. The host sequence flanking integrated HT-1 MuSV have been identified as a single EcoRI restriction fragment of 5.6 kb in normal mink cells.

show abstract

RNA Viruses, Cancer and Development

Temin

1980

Results and Problems in Cell Differentiation

View full text Add to dashboard Cite

Sites of integration of infectious DNA of avian reticuloendotheliosis viruses in different avian cellular DNAs

Cited by 39 publications

References 23 publications

Sites of integration of reticuloendotheliosis virus DNA in chicken DNA.

Sites of integration of reticuloendotheliosis virus DNA in chicken DNA.

Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.

RNA Viruses, Cancer and Development

Contact Info

Product

Resources

About