Six-Month Experience of Silver-Hydrogen Peroxide Treatment for <i>Legionella</i> Control in Two Nursing Home Water Systems

Ricci, Maria Luisa; Dell’Eva, I.; Scaturro, Maria; Baruchelli, P.; Ponte, Giulia De; Losardo, M.; Ottaviani, Massimo; Guizzardi, F.

doi:10.1128/9781555815660.ch122

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…Another two experimentations, performed in Italian long-term care facilities, did not yield a total abatement of the contamination, although an approximate 2-log reduction in Legionella mean count was attained [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Application of Hydrogen Peroxide as an Innovative Method of Treatment for Legionella Control in a Hospital Water Network

et al. 2017

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Objectives: To evaluate the effectiveness of hydrogen peroxide (HP) use as a disinfectant in the hospital water network for the control of Legionella spp. colonization. Methods: Following the detection of high levels of Legionella contamination in a 136-bed general hospital water network, an HP treatment of the hot water supply (25 mg/L) was adopted. During a period of 34 months, the effectiveness of HP on Legionella colonization was assessed. Legionella was isolated in accordance with ISO-11731 and identification was carried out by sequencing of the mip gene. Results: Before HP treatment, L. pneumophila sg 2–15 was isolated in all sites with a mean count of 9950 ± 8279 cfu/L. After one-month of HP treatment, we observed the disappearance of L. pneumophila 2–15, however other Legionella species previously not seen were found; Legionella pneumophila 1 was isolated in one out of four sampling sites (2000 cfu/L) and other non-pneumophila species were present in all sites (mean load 3000 ± 2887 cfu/L). Starting from September 2013, HP treatment was modified by adding food-grade polyphosphates, and in the following months, we observed a progressive reduction of the mean load of all species (p < 0.05), resulting in substantial disappearance of Legionella colonization. Conclusion: Hydrogen peroxide demonstrated good efficacy in controlling Legionella. Although in the initial phases of treatment it appeared unable to eliminate all Legionella species, by maintaining HP levels at 25 mg/L and adding food-grade polyphosphates, a progressive and complete control of colonization was obtained.

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Section: Discussionmentioning

confidence: 99%

Application of Hydrogen Peroxide as an Innovative Method of Treatment for Legionella Control in a Hospital Water Network

et al. 2017

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“…H 2 O 2 decomposes rapidly in different environmental conditions due to microbial catalase and peroxidase, and other than abiotic action, the decomposition is promoted by heavy metal, oxidative, and reductive reactions. It shows a broad antimicrobial spectrum and has been shown to be active against bacteria, yeast, fungi, viruses, spores, proto-, and metazoans [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Advances in Legionella Control by a New Formulation of Hydrogen Peroxide and Silver Salts in a Hospital Hot Water Network

et al. 2019

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Legionella surveillance is an important issue in public health, linked to the severity of disease and the difficulty associated with eradicating this bacterium from the water environment. Different treatments are suggested to reduce Legionella risk, however long-term studies of their efficiency are lacking. This study focused on the activity of a new formulation of hydrogen peroxide and silver salts, WTP828, in the hospital hot water network (HWN) to contain Legionella contamination during two years of treatment. The effectiveness of WTP828 was tested measuring physical-chemical and microbiological parameters such as Legionella, Pseudomonas aeruginosa (P. aeruginosa), and a heterotopic plate count (HPC) at 36 °C. Legionella isolates were identified by serotyping and genotyping. WTP 828 induced a reduction in Legionella–positive sites (60% to 36%) and contamination levels (2.12 to 1.7 log10 CFU/L), with isolates belonging to L. pneumophila SG1 (ST1 and ST104), L. anisa and L. rubrilucens widely distributed in HWN. No relevant contamination was found for other parameters tested. The long-term effect of WTP828 on Legionella containment suggest the easy and safe application of this disinfectant, that combined with knowledge of building characteristics, an adequate environmental monitoring and risk assessment plan, become the key elements in preventing Legionella contamination and exposure.

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“…Other two experimentations, performed in Italian long-term-care facilities, didn't yield a total abatement of the contamination, although a reduction in Legionella mean count of about 2 log was attained [33,34].…”

Section: Beforementioning

confidence: 89%

The Effect of Hydrogen Peroxide on Reducing the Colonization of <em>Legionella spp.</em> in a Hospital Water Network

Casini

Aquino

Totaro

et al. 2017

Preprint

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Objectives: To evaluate the effectiveness of hydrogen peroxide (HP) use in the hospital water network disinfection to control Legionella spp. colonization. Methods:Following the detection of high levels of Legionella contamination in a 136-bed general hospital water network, an HP treatment of the hot water (25 mg/L) was adopted. During a period of 34 months, the effectiveness of HP on Legionella colonization was assessed. Legionella was isolated in accordance with ISO-11731 and identification was carried out by sequencing of the mip gene.

show abstract

Six-Month Experience of Silver-Hydrogen Peroxide Treatment for Legionella Control in Two Nursing Home Water Systems

Cited by 3 publications

References 9 publications

Application of Hydrogen Peroxide as an Innovative Method of Treatment for Legionella Control in a Hospital Water Network

Application of Hydrogen Peroxide as an Innovative Method of Treatment for Legionella Control in a Hospital Water Network

Advances in Legionella Control by a New Formulation of Hydrogen Peroxide and Silver Salts in a Hospital Hot Water Network

The Effect of Hydrogen Peroxide on Reducing the Colonization of <em>Legionella spp.</em> in a Hospital Water Network

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