Size and metabolic properties of fibers in rat fast-twitch muscles after hindlimb suspension

Roy, Roland R.; Bello, M. A.; Bouissou, P.; Edgerton, V. Reggie

doi:10.1152/jappl.1987.62.6.2348

Cited by 71 publications

(35 citation statements)

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“…Arguing against this possibility, however, is the observation that fiber size did not differ significantly among groups. Previous studies have documented correlations between fiber size and metabolic capacity in rats and lizards (18,37). In a previous study of 35 male Hsd:ICR mice, we reported a positive correlation between sprint speed and maximal oxygen consumption (',¢O2max) determined during forced treadmill exercise (13).…”

Section: Discussionmentioning

confidence: 75%

Maximal sprint speeds and muscle fiber composition of wild and laboratory house mice

Garland

Gleeson

Aronovitz

et al. 1995

Physiology & Behavior

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GARLAND, T., JR., T. T. GLEESON, B. A. ARONOVITZ, C. S. RICHARDSON AND M. R. DOHM. Maximal sprint speeds and muscle fiber composition of wiM and laboratory house mice. PHYSIOL BEHAV 58(5) 869-876, 1995.--We compared males from four groups of house mice (Mus domesticus), all bred and raised under common conditions in the laboratory: randombred Hsd:ICR, a wild population from Wisconsin; hybrids from lab dams; hybrids from wild dams.. Wild mice were much faster sprinters (maximal forced sprint speeds over 1.0 m ranged from 2.38 to 3.34 m/s) than were lab mice (range = 0.89-1.68 m/s). Hybrids exhibited intermediate speeds (range = 1.54-2.70 m/s) and body masses, indicating largely additive inheritance. Type-specific mean muscle fiber cross-sectional areas of the gastrocnemius muscle did not differ significantly among groups. Percentage cross-sectional areas occupied by each of the three identified fiber types also did not differ significantly among groups, nor did they covary with body mass. For their body mass, however, lab mice had smaller gastrocnemius muscles than did wild and hybrid mice, which had muscles of similar size. Although we cannot rule out the possibility that smaller gastrocnemius muscles or slight differences in fiber composition account for the lower sprint speeds of the lab mice, we suggest that differences in unmeasured physiological, behavioral or motivational factors are probably the primary cause. This interpretation is supported by a lack of correlation between individual differences in sprint speed and either relative gastrocnemius muscle mass or muscle fiber type composition.

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Section: Discussionmentioning

confidence: 75%

Maximal sprint speeds and muscle fiber composition of wild and laboratory house mice

Garland

Gleeson

Aronovitz

et al. 1995

Physiology & Behavior

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“…So far, lots of in vitro studies have focused on the muscle energy metabolism (Baldwin et al, 1993;Desplanches et al, 1990;Fell et al, 1985;Krieger et al, 1980;Levio et al, 1998;Roy et al, 1987) of muscles affected by disuse atrophy, and have indicated that oxidative capacity decreased in the case of disuse. However, the course of the decreasing process has not been investigated as yet.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Disuse Atrophy of Rat Skeletal Muscle Based on Muscle Energy Metabolism Assessed by 31P-MRS.

Yoshida¹,

Ikata²,

Sairyo³

et al. 2001

J. Physiol. Anthropol.

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The purpose of this study was to evaluate disuse atrophy of skeletal muscle using a hind-limb suspension model, with special reference to energy metabolism. Twenty-four Sprague-Dawley rats were divided into four groups: control group (C), hind-limb suspended for 3 days (HS-3), for 7 days (HS-7) and for 14 days . The gastrocnemius-plantaris-soleus (GPS) muscles in each group were subjected to the following measurements. After a 2-min rest, contraction of the GPS muscles was induced by electrical stimulation of the sciatic nerve at 0.25 Hz for 10 min, then the frequency was increased to 0.5 and 1.0 Hz every 10 min. During the stimulation, twitch forces were recorded by a strain gauge, and 31 P-MRS was performed simultaneously. M a x i m u m t en s i o n wa s m e a s u r e d a t th e m u s cl e contraction induced at 0.25 Hz; the wet weight of the whole and each muscle in the GPS muscles was also measured. From the 31 P-MR spectra during muscle contraction, the oxidative capacity was calculated and compared among the groups. The weights of the whole GPS muscles in C, HS-3, HS-7 and HS-14, were 2.66 ± 0.09, 2.39 ± 0.21, 2.34 ± 0.21 and 2.18 ± 0.14 (g) respectively. Thus, the muscle mass significantly decreased with time (p<0.05). Among the GPS muscles, the decrease in weight of the soleus muscle was especially remarkable; in the HS-14 group its weight decreased to 60% of that in the C group. We evaluated maximum tension and oxidative capacity as the muscle function. The maximum tensions in C, HS-3, HS-7 and HS-14 were 519 ± 43, 446 ± 66, 450 ± 23 and 465 ± 29 (g), respectively. This was significantly greater in the C group than in any other groups, however there were no significant differences among the three HS g r oups . T h e oxi dat i ve c apac i t y d uri n g m usc l e contraction in the C group was higher than in any HS group and it did not further decrease even if the suspension of the limbs was prolonged beyond 3 days. The present study showed that in disuse atrophy, muscle mass and muscle function did not change simultaneously. Thus, it is necessary to develop countermeasures to p r e v e n t m u s c l e a t r o p h y a n d m u s c l e f u n c t i o n deterioration independently.

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“…TEMPLETON et al (1988) reported that following a 4-week suspension of rat hindlimbs, the percent distribution of type I fiber was reduced and that of type ha fiber was significantly increased. On the other hand, RoY et al (1987) reported that no significant changes in fiber-type distribution were observed in either the superficial region of the gastrocnemius or the tibialis anterior muscles following a 4-week hindlimb suspension in rats. These reports suggested that hindlimb suspension affected not only the functional profiles but also the structural profiles in the skeletal muscle fiber, however, skeletal muscle consists of three major types of fiber classified by contraction speed and metabolic profiles (PETER et al, 1972).…”

mentioning

confidence: 95%

“…Currently, it is not clear whether the structural and metabolic changes occurred equally or not in different types of fibers following hindlimb suspension. RoY et al (1987) and HAUSCHKA et al (1987) analyzed the size and metabolic properties of fast-twitch or slow-twitch fibers, respectively, following a 4-week hindlimb suspension in rats. They reported that the hindlimb suspension affected each type of muscle fiber differently, although the relationship between metabolic and ultrastructural profiles in each type of single muscle fiber was not examined.…”

mentioning

confidence: 99%

Ultrastructural and metabolic profiles on single muscle fibers of different types after hindlimb suspension in rats.

Takekura

Yoshioka

1989

Jpn.J.Physiol

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Abstracts The relationships between ultrastructural and metabolic profiles in different types of single muscle fiber after hindlimb suspension in rats were examined. Glycolytic (lactate dehydrogenase, LDH; phosphofructokinase, PFK) and oxidative (succinate dehydrogenase, SDH; malate dehydrogenase, MDH) enzyme activities in extensor digitorum longus (EDL) and soleus (SOL) muscles were measured. Relative mitochondrial and lipid droplet volumes were also measured in single muscle fiber of different types. Glycolytic enzyme activity in EDL muscle and oxidative enzyme activity in soleus muscle decreased following suspension for 2 weeks. LDH and PFK activities in fast-twitch (FG, fast-twitch glycolytic; FOG, fast-twitch oxidative glycolytic) fibers and oxidative enzymes in FOG and FG fibers decreased following suspension. Relative mitochondrial volume decreased significantly in all types (SO, slow-twitch oxidative; FOG, and FG) of fibers following suspension. The mitochondrial volume in SO fiber of the control group was significantly (p <0.01) higher than that of suspended group; however, SDH and MDH activities were not different between the control and suspended groups. The structural and metabolic changes following hindlimb suspension were influenced by different factors, respectively. Changes in ultrastructural and metabolic profiles in response to the hindlimb suspension differed according to the type of fibers.

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Size and metabolic properties of fibers in rat fast-twitch muscles after hindlimb suspension

Cited by 71 publications

References 0 publications

Maximal sprint speeds and muscle fiber composition of wild and laboratory house mice

Maximal sprint speeds and muscle fiber composition of wild and laboratory house mice

Evaluation of Disuse Atrophy of Rat Skeletal Muscle Based on Muscle Energy Metabolism Assessed by 31P-MRS.

Ultrastructural and metabolic profiles on single muscle fibers of different types after hindlimb suspension in rats.

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