Size and surface charge effect of layered double hydroxide particles upon blood cells

Yamaguchi, T.; Kim, Hyoung-Mi; Jung, Byung Chul; Kim, Yoon Suk; Oh, Jae‐Min

doi:10.1016/j.clay.2022.106549

Cited by 8 publications

(10 citation statements)

References 60 publications

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“…In order to comprehend the biocompatibility of layered double hydroxide (LDH) nanomaterials to whole blood, the interactions between the LDHs with blood proteins were investigated, adding to the previous research on LDH–blood cell interaction [ 34 ]. The behavior of LDH depending on particle size and surface charge was investigated by adsorption isotherms and fluorescence quenching.…”

Section: Discussionmentioning

confidence: 99%

“…The situation was similar in LDH-based delivery carriers; increasing demand on development of the injectable LDH carrier was not satisfied by the research on hematocompatibility of the LDH nanoparticles [ 29 , 30 , 31 , 32 , 33 ]. According to a recent study [ 34 ], a LDH with a diameter of 2000 nm showed only 2% hemolysis after 24 h incubation, while the smaller ones did not show significant effect, suggesting high hematocompatibility of LDHs. In order to comprehend LDH–blood interactions in detail, it is highly recommended to investigate interaction between LDHs and blood plasma proteins.…”

Section: Introductionmentioning

confidence: 99%

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Photochemical Consideration in the Interactions between Blood Proteins and Layered Inorganic Materials

Yamaguchi

Kim

2022

IJMS

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Interactions between layered double hydroxide (LDH) nanomaterials and plasma proteins according to their particle size and surface charge were evaluated. The LDHs with different particle size (150, 350 and 2000 nm) were prepared by adjusting hydrothermal treatment and urea hydrolysis and subsequent organic coating with citrate, malite and serite was applied to control the surface charge (ζ-potential: −15, 6 and 36 mV). Adsorption isotherms and Stern–Volmer plots for fluorescence quenching indicated that the human blood plasma had weak interactions toward all the types of LDHs. The adsorption isotherms did not show significant differences in the size and surface charges, while the fluorescence quenching ratio increased with the increase in the surface charge, implying that electrostatic interaction played a major role in their interactions. The fluorescence quenching of three types of plasma proteins (human serum albumin, γ-globulin and fibrinogen) by the surface charge-controlled LDHs suggested that the proteins adsorbed on the LDHs with a single layer and additional proteins were weakly adsorbed to surround the LDHs with adsorbed proteins. It was concluded that the LDH nanomaterials are fairly compatible for blood components due to the protein corona while the electrostatic interaction can affect their interaction with the proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photochemical Consideration in the Interactions between Blood Proteins and Layered Inorganic Materials

Yamaguchi

Kim

2022

IJMS

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“…Taking into account the previous studies on LDH alone, the blood compatibility of MTX-LDH was researched in terms of the blood cell interaction and protein interaction. As we reported previously [18], we hypothesized that the MTX-LDH hybrid can also take advantage of RBC hitchhiking and the protein corona effect (Scheme 1). The RBCs' interaction with MTX-LDH was investigated by hemolysis and the cell counting assay.…”

Section: Introductionmentioning

confidence: 94%

“…Despite the growing interest in LDH as a DDS, there has been only limited information on the hematocompatibility of LDH. We recently reported that LDH itself demonstrated negligible blood cell damage regardless of the particle size surface charge [18] and that LDH did not seriously denature the plasma proteins [11,19]. Other research also reported that surface modification of LDH with polysulfobetaine had excellent blood compatibility [20,21].…”

Section: Introductionmentioning

confidence: 97%

“…Landgraf et al presented that the gold nanoparticleincorporated Fe 3 O 4 with protein corona had enhanced biocompatibility in mouse systems compared with corona-free ones [30]. Meanwhile, we previously reported that LDH nanomaterials were fairly inert to red blood cells (RBCs) regardless of the size and surface charge [18], and that LDH could interact with proteins to different degrees depending on the size and surface charge [19]. The drug-incorporated LDH, of which the surface might be partially covered by the drug, would undergo different blood interactions [11].…”

Section: Introductionmentioning

confidence: 99%

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Blood Compatibility of Drug–Inorganic Hybrid in Human Blood: Red Blood Cell Hitchhiking and Soft Protein Corona

Xie,

Kim,

Kamada

et al. 2023

Materials

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A drug-delivery system consisting of an inorganic host—layered double hydroxide (LDH)—and an anticancer drug—methotrexate (MTX)—was prepared via the intercalation route (MTX-LDH), and its hematocompatibility was investigated. Hemolysis, a red blood cell counting assay, and optical microscopy revealed that the MTX-LDH had no harmful toxic effect on blood cells. Both scanning electron microscopy and atomic force microscopy exhibited that the MTX-LDH particles softly landed on the concave part inred blood cells without serious morphological changes of the cells. The time-dependent change in the surface charge and hydrodynamic radius of MTX-LDH in the plasma condition demonstrated that the proteins can be gently adsorbed on the MTX-LDH particles, possibly through protein corona, giving rise to good colloidal stability. The fluorescence quenching assay was carried out to monitor the interaction between MTX-LDH and plasma protein, and the result showed that the MTX-LDH had less dynamic interaction with protein compared with MTX alone, due to the capsule moiety of the LDH host. It was verified by a quartz crystal microbalance assay that the surface interaction between MTX-LDH and protein was reversible and reproducible, and the type of protein corona was a soft one, having flexibility toward the biological environment.

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Effects of charges of Eu3+-doped LDH laminates on drug loading capacity and drug release as well as its anticancer effects

Li,

Shang,

Liu

et al. 2023

Res Chem Intermed

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Size and surface charge effect of layered double hydroxide particles upon blood cells

Cited by 8 publications

References 60 publications

Photochemical Consideration in the Interactions between Blood Proteins and Layered Inorganic Materials

Photochemical Consideration in the Interactions between Blood Proteins and Layered Inorganic Materials

Blood Compatibility of Drug–Inorganic Hybrid in Human Blood: Red Blood Cell Hitchhiking and Soft Protein Corona

Effects of charges of Eu3+-doped LDH laminates on drug loading capacity and drug release as well as its anticancer effects

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