Size, conformation and purity of chloroplast DNA of some higher plants

Herrmann, Reinhold G.; Bohnert, H. J.; Kowallik, Klaus V.; Schmitt, Jürgen

doi:10.1016/0005-2787(75)90118-5

Cited by 118 publications

(38 citation statements)

References 19 publications

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“…This low recovery of circular DNA forms might result from a transition from the folded molecule directly to a nicked linear form which is induced by high salt core stripping. The fact (20) Folding of ctDNA may not be entirely dependent upon the presence of a core structure. When membrane-free E. coli chromosomes are isolated, an RNase sensitive site is apparent (36).…”

Section: Discussionmentioning

confidence: 99%

“…This limited information exists as the only data on the chloroplast chromosome unit size for any member of this important evolutionary line of plants. In contrast to the paucity of information on ctDNA3 structure among the Chromophyta, considerable data is available from a wide variety of Chlorophyta, those plants which contain both Chl a and b. Chloroplasts of the Chlorophyta contain multiple DNA copies (4,18) whose unit chromosome is a circular molecule (20,26). Land 3 Abbreviations: ctDNA, chloroplast DNA; Sarkosyl, sodium n-laurol sarcosinate; ATA, aurintricarboxylic acid.…”

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confidence: 99%

“…Land 3 Abbreviations: ctDNA, chloroplast DNA; Sarkosyl, sodium n-laurol sarcosinate; ATA, aurintricarboxylic acid. contour length (22) for liverwort, 43 to 45 ,Im for fern (22), and 38 and 46 ,im for angiosperm species (20,26) such as corn, oats, pea, snapdragon, and evening primrose. Chloroplast DNA contour lengths of four chlorophytic algal representatives have been successfully analyzed.…”

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confidence: 99%

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Isolation and Characterization of Chloroplast DNA from the Marine Chromophyte, Olisthodiscus luteus: Electron Microscopic Visualization of Isomeric Molecular Forms

Aldrich¹,

Cattolico²

1981

Plant Physiol.

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Chloroplast DNA (ctDNA) from the marine chromophytic alga, Olisthodiscus luteus, has been isolated using a whole cel lysis method folowed by CsCI-Hoechst 33258 dye gradient centrifugation. This DNA, which has a buoyant density of 1.691 grams per cubic centimeter was identified as plastidic in origin by enrichment experiments. Inclusion of the nuclease inhibitor aurintricarboxylic acid in all lysis buffers was mandatory for isolation of high molecular weight DNA. Long linear molecules (40 to 48 micrometers) with considerable internal organization comprised the majority of the ctDNA isolated, whereas supertwisted ctDNA and open circular molecules averaging 46 micrometers were occasionally present. Also observed in this study were folded ctDNA molecules with electron dense centers ("rosettes") and plastid DNA molecules which have a tightly wound "key-ring" center. The ctDNA of Olisthodiscus has a contour length that is median to the size range reported for chiorophytic plants.A minor component of the total celular DNA, which originates from a DNase insensitive cellular structure, has a buoyant density of 1.694 grams per cubic centimeter. This DNA consists predominantly oflinear molecules, but open circles 11.5 micrometers in length and rare 22-micrometer dimers were also present.This study represents the first analysis of the extranuclear DNA of a chromophytic alga.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Isolation and Characterization of Chloroplast DNA from the Marine Chromophyte, Olisthodiscus luteus: Electron Microscopic Visualization of Isomeric Molecular Forms

Aldrich¹,

Cattolico²

1981

Plant Physiol.

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“…This report established the Broken Circles theory for cpDNA that has dominated the field until the present day. Using similar procedures for cpDNA isolation and EM with several plant species, Herrmann et al (1975) found as much as 80% circular cpDNA molecules in some preparations, and they too adopted the Broken Circles theory. EM was again used to provide additional examples of plants with circular cpDNA (Kolodner and Tewari, 1975a).…”

Section: Chromosomal Dna Before the Identification Of Chloroplast Dnamentioning

confidence: 99%

Circular Chloroplast Chromosomes: The Grand Illusion

2004

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“…Manning et al (1971) observed the first circular DNA molecules in lysates of Euglena chloroplasts. Large circular DNA molecules have since been obtained from chloroplasts of several higher plant species (Kolodner and Tewari 1975;Herrmann et al 1975).…”

Section: Discovery Of Chloroplast Dnamentioning

confidence: 99%

History of chloroplast genomics

Sugiura

Discoveries in Photosynthesis

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The presence of chloroplast DNA was established in 1963. With the development of recombinant DNA technologies, chloroplast DNA was selected as one of the first candidates for genome sequencing. The first physical map was reported for maize chloroplasts in 1976. As tobacco has been popular as an experimental system, tobacco chloroplast DNA has been extensively analyzed and the complete nucleotide sequence was established in 1986. This sequencing and the availability of tobacco chloroplast transformation techniques and of in vitro expression systems have formed the basis of an ongoing functional genomics program.

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Size, conformation and purity of chloroplast DNA of some higher plants

Cited by 118 publications

References 19 publications

Isolation and Characterization of Chloroplast DNA from the Marine Chromophyte, Olisthodiscus luteus: Electron Microscopic Visualization of Isomeric Molecular Forms

Isolation and Characterization of Chloroplast DNA from the Marine Chromophyte, Olisthodiscus luteus: Electron Microscopic Visualization of Isomeric Molecular Forms

Circular Chloroplast Chromosomes: The Grand Illusion

History of chloroplast genomics

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