Size-controlled biodegradable nanoparticles: Preparation and size-dependent cellular uptake and tumor cell growth inhibition

Choi, Jin‐Seok; Cao, Jiafu; Naeem, Muhammad; Noh, Jin-Ki; Hasan, Nurhasni; Choi, Hoo‐Kyun; Yoo, Jin‐Wook

doi:10.1016/j.colsurfb.2014.07.030

Cited by 112 publications

(70 citation statements)

References 24 publications

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“…The phenomenon of particle size-dependent uptake has been a subject of study. However, the results of their studies differ [47][48][49]. Most researchers agreed that the shortest wrapping time depends on the particle size.…”

Section: In Vitro Cellular Uptakementioning

confidence: 94%

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

et al. 2015

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Abstract. This study was conducted to develop formulations of hydrocortisone butyrate (HB)-loaded poly(D,L-lactic-co-glycolic acid) nanoparticles (PLGA NP) suspended in thermosensitive gel to improve ocular bioavailability of HB for the treatment of bacterial corneal keratitis. PLGA NP with different surfactants such as polyvinyl alcohol (PVA), pluronic F-108, and chitosan were prepared using oil-in-water (O/W) emulsion evaporation technique. NP were characterized with respect to particle size, entrapment efficiency, polydispersity, drug loading, surface morphology, zeta potential, and crystallinity. In vitro release of HB from NP showed a biphasic release pattern with an initial burst phase followed by a sustained phase. Such burst effect was completely eliminated when nanoparticles were suspended in thermosensitive gels and zero-order release kinetics was observed. In HCEC cell line, chitosanemulsified NP showed the highest cellular uptake efficiency over PVA-and pluronic-emulsified NP (59.09±6.21%, 55.74±6.26%, and 62.54±3.30%, respectively) after 4 h. However, chitosan-emulsified NP indicated significant cytotoxicity of 200 and 500 μg/mL after 48 h, while PVA-and pluronic-emulsified NP exhibited no significant cytotoxicity. PLGA NP dispersed in thermosensitive gels can be considered as a promising drug delivery system for the treatment of anterior eye diseases.

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Section: In Vitro Cellular Uptakementioning

confidence: 94%

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

et al. 2015

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“…We selected PLGA as the basic polymer because it is an established biocompatible and biodegradable polymer that has long been used for controlled release drug delivery. 23,24 PEI was selected as the NO donor polymer. The PEI/diazeniumdiolate (PEI/NONOate) was synthesized by reacting NO with the secondary amine groups of PEI and incorporated in the matrix of PLGA NPs.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the comparatively prolonged NO release of our NO/PPNPs would be beneficial for biomedical applications such as antibacterial activity, which requires sustained NO release. Since drug release from the PLGA NPs can be controlled by changing the polymer hydrophobicity and particle size, 23 NO release from NO/PPNPs could be further optimized.…”

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confidence: 99%

Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity

Hasan¹,

Cao²,

Choi³

et al. 2015

IJN

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Nitric oxide (NO)-releasing nanoparticles (NPs) have emerged as a wound healing enhancer and a novel antibacterial agent that can circumvent antibiotic resistance. However, the NO release from NPs over extended periods of time is still inadequate for clinical application. In this study, we developed NO-releasing poly(lactic- co -glycolic acid)-polyethylenimine (PEI) NPs (NO/PPNPs) composed of poly(lactic- co -glycolic acid) and PEI/diazeniumdiolate (PEI/NONOate) for prolonged NO release, antibacterial efficacy, and wound healing activity. Successful preparation of PEI/NONOate was confirmed by proton nuclear magnetic resonance, Fourier transform infrared spectroscopy, and ultraviolet/visible spectrophotometry. NO/PPNPs were characterized by particle size, surface charge, and NO loading. The NO/PPNPs showed a prolonged NO release profile over 6 days without any burst release. The NO/PPNPs exhibited potent bactericidal efficacy against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa concentration-dependently and showed the ability to bind on the surface of the bacteria. We also found that the NO released from the NO/PPNPs mediates bactericidal efficacy and is not toxic to healthy fibroblast cells. Furthermore, NO/PPNPs accelerated wound healing and epithelialization in a mouse model of a MRSA-infected wound. Therefore, our results suggest that the NO/PPNPs presented in this study could be a suitable approach for treating wounds and various skin infections.

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“…Particle size is a key parameter for drug and nutrition delivery, as the size can significantly influence the interactions of particles with cells and therefore their absorption and distribution in vivo. 27 Moreover, key factors and related mechanisms were also investigated and discussed. As far as we know, this study is the first report on size-controlled fabrication of zein particles through adjusting a single factor.…”

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confidence: 99%

Size-controlled fabrication of zein nano/microparticles by modified anti-solvent precipitation with/without sodium caseinate

Li¹,

Yan²,

Liu³

et al. 2017

IJN

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Zein-based nano/microparticles have been demonstrated to be promising carrier systems for both the food industry and biomedical applications. However, the fabrication of size-controlled zein particles has been a challenging issue. In this study, a modified anti-solvent precipitation method was developed, and the effects of various factors, such as mixing method, solvent/anti-solvent ratio, temperature, zein concentrations and the presence of sodium caseinate (SC) on properties of zein particles were investigated. Evidence is presented that, among the previously mentioned factors, the mixing method, especially mixing rate, could be used as an effective parameter to control the size of zein particles without changing other parameters. Moreover, through fine-tuning the mixing rate together with zein concentration, particles with sizes ranging from nanometers to micrometers and low polydispersity index values could be easily obtained. Based on the size-controlled fabrication method, SC-coated zein nanoparticles could also be obtained in a size-controlled manner by incubation of the coating material with the already-formed zein particles. The resultant nanoparticles showed better performance in both drug loading and controlled release, compared with zein/SC hybrid nanoparticles fabricated by adding aqueous ethanol solution to SC solution. The possible mechanisms of the nanoprecipitation process and self-assembly formation of these nanoparticles are discussed.

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Size-controlled biodegradable nanoparticles: Preparation and size-dependent cellular uptake and tumor cell growth inhibition

Cited by 112 publications

References 24 publications

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity

Size-controlled fabrication of zein nano/microparticles by modified anti-solvent precipitation with/without sodium caseinate

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Size-controlled biodegradable nanoparticles: Preparation and size-dependent cellular uptake and tumor cell growth inhibition

Cited by 112 publications

References 24 publications

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and&nbsp;in&nbsp;vivo wound healing activity

Size-controlled fabrication of zein nano/microparticles by modified anti-solvent precipitation with/without sodium caseinate

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Nitric oxide-releasing poly(lactic-co-glycolic acid)-polyethylenimine nanoparticles for prolonged nitric oxide release, antibacterial efficacy, and in vivo wound healing activity