Size‐dependent light scattering of CoOOH nanoflakes for convenient and sensitive detection of alkaline phosphatase in human serum

Abstract

As a natural enzyme, alkaline phosphatase (ALP) plays an essential role in clinicopathological examinations and biomedical research, and is capable of hydrolyzing the phosphate group of l‐ascorbic acid‐2‐phosphate (AAP) to yield l‐ascorbic acid (L‐AA). L‐AA reduced cobalt oxyhydroxide (CoOOH) nanoflakes to Co2+, leading to a smaller size and weaker light scattering, which could be monitored by electron microscopic images and optical spectra. The indirect detection of ALP was achieved by the reduced light scattering signal of CoOOH nanoflakes. Under optimal conditions, the decrease in scattering intensity was proportional to the ALP concentration over the range 0.1–160 U/L and the detection limit was 0.034 U/L (3σ/k). Compared with other assays, this proposed light scattering method was more convenient and economic for ALP sensing. The method was successfully applied to ALP analysis in human serum samples, and was similar to the results obtained by commercial kits.

Silver-Coated Gold Nanorods as Optical Probes for the Sensitive Detection of Ascorbic Acid in Tablets

“…For example, traditional iodometric titration [4] has been employed in pharmacopoeias. In recent years, modern technical methods have been proposed, such as light scattering [5], colorimetry [6,7], fluorimetry [8], electrochemistry [9,10], and chemiluminescence [11]. Among them, absorption spectrometry has attracted widespread concern owing to its low cost, easy operation, and flexible probes [12].…”