Size-Dependent MRI Relaxivity and Dual Imaging with Eu0.2Gd0.8PO4·H2O Nanoparticles

Li, Yichen; Chen, Tao; Tan, Weihong; Talham, Daniel R.

doi:10.1021/la500602x

Cited by 27 publications

(21 citation statements)

References 43 publications

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“…174 Talham and co-workers synthesized three different sizes of Eu 0.2 Gd 0.8 PO 4 ·H 2 O nanoparticles and found that r 1 increased for smaller particles. 185 Rahman and coworkers synthesized Gd 2 O 3 nanoparticles with the size varying from 1.5 to 194.0 nm. The nanoparticles with size of 2.3 nm showed the highest r 1 , while r 1 drastically decreased for other nanoparticles with smaller and larger sizes, indicating there is an optimum Gd 2 O 3 nanoparticle size of 2.3 nm.…”

Section: 2mentioning

confidence: 99%

Lanthanide Nanoparticles: From Design toward Bioimaging and Therapy

et al. 2015

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Section: 2mentioning

confidence: 99%

Lanthanide Nanoparticles: From Design toward Bioimaging and Therapy

et al. 2015

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“…137 GdF 3 NPs doped with Er 3+ /Yb 3+ and Tm 3+ /Yb 3+ have also been proposed as bimodal probes for both in vitro and in vivo optical and MRI, the latter technique taking advantage from the high R 2 relaxivity found for these hybrid NPs. In principle, the combination of fluorescent bioimaging with MRI capabilities is appealing because the greater sensitivity and resolution of fluorescence imaging may complement MRI data.…”

Section: View Article Onlinementioning

confidence: 99%

Imaging agents based on lanthanide doped nanoparticles

et al. 2015

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Nanotechnology has recently allowed us to design and prepare nanoplatforms with the potential to face currently unresolved problems. Among these platforms, nanoparticles in particular are versatile objects that find applications in many different areas. In the vast ensemble of materials that have been explored to obtain nanoparticles with improved performances, we here focus our attention on lanthanide-based nanocrystals. These recently developed species are extremely interesting and well known particularly for their ability to emit anti-Stokes shifted light (upconversion) with relatively high brightness. Many advantageous characteristics of such materials are emerging, and their use as multimodal imaging agents is rapidly growing. We here survey some recent examples on this subject, mainly focusing on systems having NIR-to-NIR emission properties for in vivo applications.

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“…The corresponding T2 values were 56.6 ms for the control, 47.5 ms (labeling with 10 µg Fe/mL), 36.8 ms (labeling with 30 µg Fe/mL) and 23.2 ms (labeling with 50 µg Fe/mL). These results show adequate internalization and, due to the M-SPIONs, a high r2/r1 ratio of 165.8 (r2 = (19.9 ± 0.9) × 10 −4 ms −1 µg Fe −1 mL and r1 = (1.2 ± 0.5) × 10 −5 ms −1 µg Fe −1 mL), indicating a strong contrast in T2-weighted images [95][96][97][98][99]. In relation to the NIRF qualitative analysis, the NIRF intensity signal was possible due to the M-SPIONs used for granulocyte-labeling having a fluorophore coupled to the infrared wavelength.…”

Section: Discussionmentioning

confidence: 74%

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

et al. 2020

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This in vitro study aimed to find the best method of granulocyte isolation for subsequent labeling with multimodal nanoparticles (magnetic and fluorescent properties) to enable detection by optical and magnetic resonance imaging (MRI) techniques. The granulocytes were obtained from venous blood samples from 12 healthy volunteers. To achieve high purity and yield, four different methods of granulocyte isolation were evaluated. The isolated granulocytes were labeled with multimodal superparamagnetic iron oxide nanoparticles (M-SPIONs) coated with dextran, and the iron load was evaluated qualitatively and quantitatively by MRI, near-infrared fluorescence (NIRF) and inductively coupled plasma mass spectrometry (ICP-MS). The best method of granulocyte isolation was Percoll with Ficoll, which showed 95.92% purity and 94% viability. After labeling with M-SPIONs, the granulocytes showed 98.0% purity with a yield of 3.5 × 10 6 cells/mL and more than 98.6% viability. The iron-loading value in the labeled granulocytes, as obtained by MRI, was 6.40 ± 0.18 pg/cell. Similar values were found with the ICP-MS and NIRF imaging techniques. Therefore, our study shows that it is possible to isolate granulocytes with high purity and yield and labeling with M-SPIONs provides a high internalized iron load and low toxicity to cells. Therefore, these M-SPION-labeled granulocytes could be a promising candidate for future use in inflammation/infection detection by optical and MRI techniques.Hard-to-reach or occult inflammation and infection processes are clinically challenging. Their clinical diagnoses involve biochemical and radiological examinations, but these methods can produce false negatives [11][12][13][14][15]. To determine the localization, extent and severity of inflammation, imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI) can be performed but have limitations in the early stages of the disease, as they are dependent on morphological changes [16][17][18]. Accurate and early diagnosis of inflammation and infection helps reduce mortality and morbidity and increase drug treatment success [19][20][21].Proinflammatory effects help leukocytes migrate from the blood vessel to the site of injury, due to increased vascular permeability through stimulation by cytokines and interleukins. There is an initial accumulation of granulocytes, mainly neutrophils, in the inflamed area and later of lymphocytes and macrophages [22][23][24]. Granulocytes are the most abundant subset of leukocytes [25] and are produced in the bone marrow, originating from myeloid precursor cells and undergoing a maturing process before they are able to perform their functions, mainly phagocytosis [25,26]. They differ from other cells in that they have granules with proteolytic enzymes that fight microorganisms or other inflammatory agents [25,27]. Granulocytes are considered frontline cells in inflammation and infection processes [28]. In many cases, to detect inflammation/infection, it is necessary to label isolated granulocyte...

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Size-Dependent MRI Relaxivity and Dual Imaging with Eu_0.2Gd_0.8PO₄·H₂O Nanoparticles

Cited by 27 publications

References 43 publications

Lanthanide Nanoparticles: From Design toward Bioimaging and Therapy

Lanthanide Nanoparticles: From Design toward Bioimaging and Therapy

Imaging agents based on lanthanide doped nanoparticles

Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles

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