1993
DOI: 10.1007/bf02272182
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Size exclusion chromatographic characterization of sodium hyaluronate fractions prepared by high energetic sonication

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Cited by 23 publications
(9 citation statements)
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“…According to Bothner and Wich [21], high viscosity hyaluronate solutions must therefore be based on high molecular weight material and a twofold increase in molecular weight results in a 10-fold increase in bulk viscosity. Controlled progressive cleavage of hyaluronan chains can be induced by ultrasound [14]. The column retention time, T r (in minutes), was found to increase as a negative exponential function of the sonication time, indicating progressive limited cleavage of the chains (Fig.…”
Section: Resultsmentioning
confidence: 95%
See 1 more Smart Citation
“…According to Bothner and Wich [21], high viscosity hyaluronate solutions must therefore be based on high molecular weight material and a twofold increase in molecular weight results in a 10-fold increase in bulk viscosity. Controlled progressive cleavage of hyaluronan chains can be induced by ultrasound [14]. The column retention time, T r (in minutes), was found to increase as a negative exponential function of the sonication time, indicating progressive limited cleavage of the chains (Fig.…”
Section: Resultsmentioning
confidence: 95%
“…Several research groups agree that ultrasound does not allow complete degradation of HA chains into monomers [1]. The mechanism and kinetics are not yet well understood and different relationships between the Mw of HA and the reaction time have been observed [12][13][14]. Sonication appears to degrade HA in a non-random fashion that results in constant bimodal molecular weight distribution.…”
Section: Introductionmentioning
confidence: 91%
“…Here, ultrasonication was used to mechanically break down the hyaluronan network within synovial fluid in a controlled manner (Orvisky 1993). As sonication time increased, synovial fluid viscosity decreased.…”
Section: Discussionmentioning
confidence: 99%
“…After each increment, a 12 mL aliquot was collected. Using this serial sonication technique, aliquots were generated at time points ranging from 10 seconds to 200 seconds, each with a progressively degraded polysaccharide network (Orvisky 1993). To mimic the extremes of synovial fluid degradation, native synovial fluid and enzymatically degraded synovial fluid were included as controls.…”
Section: Experimental Methodsmentioning
confidence: 99%
“…The sample-5 mg of native high molecular weight hyaluronandissolved in 10mL of the HPLC eluent was exposed to high energy ultrasound (150 W; 20 kHz) generated by a UGA 20452 device (Tesla, Vrable, Slovak Republic). The molecular characteristics of hyaluronan degraded for 5, 15,30,45 or 75 min were determined by HPLC (Chabretek et al, 1990b, 1191aOrvisky et al, 1993). Figure 4 illustrates the original chromatographic records of a sample pair-native pleuran and pleuran denatured by DMSO.…”
Section: Introductionmentioning
confidence: 99%