Size Exclusion Chromatography of Dextrans in Dmso as Eluent

Merienne, S.; Busnel, Jean‐Pierre; Fricoteaux, Florence; Prudhomme, Jean-Claude

doi:10.1081/jlc-100100449

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…The refractive index increments of many polysaccharides in commonly used SEC solvents have not been extensively studied. DMSO is a better solvent than water for polysaccharides such as dextrans and xylans due to the aggregation of polysaccharides in water , . In addition, DMSO is a strong hydrogen-bond acceptor.…”

Section: Discussionmentioning

confidence: 99%

Structural Comparison of Arabinoxylans from Two Barley Side-Stream Fractions

Pitkänen

Tuomainen

Virkki

et al. 2008

J. Agric. Food Chem.

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The structures of barley ( Hordeum vulgare) arabinoxylans isolated from two industrial side fractions, barley husks (BH) and barley fiber (BF), were characterized. Arabinoxylans were extracted with saturated barium hydroxide after enzymatic pretreatment. Barium hydroxide was selective toward arabinoxylans, and only a minor amount of glucose-containing material was coextracted. Acid methanolysis followed by gas chromatography, 1H NMR spectroscopy, and specific enzymatic treatments followed by anion exchange chromatography with pulse amperometric detection (HPAEC-PAD) revealed that the chemical structure of barley husk arabinoxylan (BHAX) clearly differed from that of barley fiber arabinoxylan (BFAX). BFAX was more branched, containing more beta-D-xylopyranosyl (beta-D-Xylp) residues carrying alpha-L-arabinofuranosyl (alpha-L-Araf) units at both O-2 and O-3 positions. BHAX, on the other hand, contained more 2-O-beta-D-Xyl p-alpha-L-Ara f substituents than BFAX. BHAX and BFAX also differed with respect to the hydrodynamic properties investigated with multidetector size exclusion chromatography. BFAX had a higher weight-average molar mass and larger hydrodynamic volume, the latter indicating less dense conformation than BHAX. Mn, Mw /Mn, Rh, and the Mark-Houwink a value were also determined for both arabinoxylans.

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Section: Discussionmentioning

confidence: 99%

Structural Comparison of Arabinoxylans from Two Barley Side-Stream Fractions

Pitkänen

Tuomainen

Virkki

et al. 2008

J. Agric. Food Chem.

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“…The separation of amylopectin was unexpected as its molecular weight (up to 10 8 ) is far higher than the exclusion limit of the column. This may have been due to electrostatic repulsion between amylopectin and the gel used in the column, as already observed for dextrans in certain elution conditions (Merienne et al ., ) which may accelerate elution of the amylopectin. With normal maize, P1 and P2 were observed and the area of the first peak was twice as large as that of the second, as expected from the relative amounts of amylopectin and amylose.…”

Section: Resultsmentioning

confidence: 99%

Dextrin, sugar and organic acid profiles of spontaneous and modified gowe: a malted and fermented sorghum beverage from Benin

Vieira‐Dalodé

Akissoé

Hounhouigan

et al. 2014

Int J of Food Sci Tech

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Gow e is a traditional Beninese fermented red sorghum beverage made from a blend of malted and precooked nonmalted flour. The main objective of this work was to evaluate the degradation of starch during the production of gow e and to characterise the organic acid and sugar profiles of the product obtained using starter cultures. Inoculation was performed with strains of Lactobacillus fermentum or Weissella confusa and of Kluyveromyces marxianus or Pichia anomala used singly or in combination. Size exclusion chromatography revealed that starch was hydrolysed into high molecular weight dextrins (DP over 35), oligosaccharides and glucose, mainly due to the malting and precooking steps. Glucose was the main free sugar, and lactic acid was the main organic acid. The choice of the inoculation strain directly influenced the acidity but also indirectly the sugar content of the resulting gow e and will thus affect consumer acceptability.

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“…Another approach is in-situ pretreatment of crystalline cellulose in an ionic liquid or non-derivatizing solvent. [71][72][73] As it has been discussed extensively, [74][75][76] ionic liquid will deconstruct the crystallinity of the cellulose to a far less crystalline structure.…”

Section: Subcritical and Supercritical Watermentioning

confidence: 99%

Reaction kinetics of cellulose hydrolysis in subcritical and supercritical water

Olanrewaju¹

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The uncertainties in the continuous supply of fossil fuels from the crisis-ridden oil-rich region of the world is fast shifting focus on the need to utilize cellulosic biomass and develop more efficient technologies for its conversion to fuels and chemicals. One such technology is the rapid degradation of cellulose in supercritical water without the need for an enzyme or inorganic catalyst such as acid. This project focused on the study of reaction kinetics of cellulose hydrolysis in subcritical and supercritical water. Cellulose reactions at hydrothermal conditions can proceed via the homogeneous route involving dissolution and hydrolysis or the heterogeneous path of surface hydrolysis. The work is divided into three main parts. First, the detailed kinetic analysis of cellulose reactions in micro-and tubular reactors was conducted. Reaction kinetics models were applied, and kinetics parameters at both subcritical and supercritical conditions were evaluated. The second major task was the evaluation of yields of water soluble hydrolysates obtained from the hydrolysis of cellulose and starch in hydrothermal reactors. Lastly, changes in molecular weight distribution due to hydrothermolytic degradation of cellulose were investigated. These changes were also simulated based on different modes of scission, and the pattern generated from simulation was compared with the distribution pattern from experiments. For a better understanding of the reaction kinetics of cellulose in subcritical and supercritical water, a series of reactions was conducted in the microreactor. Hydrolysis of cellulose was performed at subcritical temperatures ranging from 270 to 340 °C (τ = 0.40-0.88 s). For the dissolution of cellulose, the reaction was conducted at supercritical temperatures ranging from 375 to 395 °C (τ = 0.27-0.44 s). The operating pressure for vii TABLE OF CONTENTS LIST OF TABLES .

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Size Exclusion Chromatography of Dextrans in Dmso as Eluent

Abstract: consider that the dextrans were primarily eluted by means of size exclusion.

Cited by 5 publications

References 15 publications

Structural Comparison of Arabinoxylans from Two Barley Side-Stream Fractions

Structural Comparison of Arabinoxylans from Two Barley Side-Stream Fractions

Dextrin, sugar and organic acid profiles of spontaneous and modified gowe: a malted and fermented sorghum beverage from Benin

Reaction kinetics of cellulose hydrolysis in subcritical and supercritical water

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