Skeletal growth and bone mineral acquisition in type 1 diabetic children; abnormalities of the GH/IGF-1 axis

Raisingani, Manish; Preneet, Brar; Kohn, Brenda; Yakar, Shoshana

doi:10.1016/j.ghir.2017.04.003

Cited by 55 publications

(53 citation statements)

References 127 publications

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“…Aşırı kilolu ve Tip 1 DM'li hastalarda kan leptin miktarını araştıran bir çalışmada DM'li grupta leptin ifadesinin anlamlı bir şekilde düştüğü saptanmıştır (25). Bu konuda yapılan çalışmaların bazısı Tip 1 DM durumunda leptin ifadesinin değişmediğini (26), bazısı ise arttığını göstermiştir (27)(28)(29).…”

Section: Discussionunclassified

Ti̇p 1 Di̇yabetes Melli̇tus Oluşturulan Siçanlarin Beyni̇nde Lepti̇n Mi̇ktari Azalmaktadir

Şahin¹,

Özcan²,

Bektur³

et al. 2019

Kocatepe Tıp Dergisi

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Leptin hormonu, iştah ve vücut metabolizmasının düzenlenmesinde önemli görevler üstlenmekte ve başlıca yağ dokusunda sentezlenmektedir. Leptinin yağ dokuda sentezlendikten sonra koroid pleksus aracılığı ile beyne taşındığı bilinmektedir. Bu çalışmanın amacı Tip 1 diyabetes mellitus oluşturulan sıçanların beyninde leptin ifadesinin ve miktarının araştırılmasıdır. GEREÇ VE YÖNTEM: Çalışmamızda toplam 14 adet yetişkin, erkek Wistar Albino sıçan 2 eşit gruba ayrıldı (n=7). Kontrol grubuna hiçbir uygulama yapılmadı. Diyabetes mellitus grubundaki hayvanlara ise tek doz (55 mg/kg) streptozotosin intraperitoneal olarak verildi ve kan glukoz seviyesi >280 mg/dL ölçüldüğünde Tip 1diyabetes mellitus geliştiği kabul edildi. Deney sonunda alınan beyin örnekleri %10'luk formaldehit ile fikse edildikten sonra rutin doku takip işleminin ardından alınan kesitlere leptin immünohistokimyasal boyaması uygulandı. Beyin dokularında western blot yöntemi ile leptin miktarına bakıldı. BULGULAR: Tüm gruplara ait beyin kesitlerinde sadece koroid pleksusta leptin boyanması saptandı. Buna göre, leptin boyanmasının Tip 1 diyabetes mellitus geliştirilen sıçanlarda azaldığı saptandı. Western blot ile Tip 1 diyabetes mellitus grubunda leptin miktarının belirgin olarak azaldığı saptandı. SONUÇ: Bu çalışmayla tokluk hissini oluşturmak üzere koroid pleksus üzerinden beyne geçen leptin hormonu ile diyabet arasında yakın bir ilişki olduğu ve bu hormonun diyabetle azaldığı gösterilmiştir. Bu çalışmadan yola çıkarak Tip 1 diyabet ve leptin hormonu ilişkisi derinlemesine irdelenmelidir.

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Section: Discussionunclassified

Ti̇p 1 Di̇yabetes Melli̇tus Oluşturulan Siçanlarin Beyni̇nde Lepti̇n Mi̇ktari Azalmaktadir

Şahin¹,

Özcan²,

Bektur³

et al. 2019

Kocatepe Tıp Dergisi

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“…Відомо, що хронічна гіперглікемія викликає пригнічення остеобластів, а також неферментативне глікування білків, що в подальшому веде до порушення їх функції [6,7].…”

Section: вступunclassified

Microscopic Structure and Morphometric Parametres of Long Tubular Bones of Senile Rats With Induced Hyperglycemia

Ponyrko

2019

Act. Probl. of the Modern Med.

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Recent studies have shown that patients with diabetes mellitus are found very often to have pathological changes in the musculoskeletal system. Discussing the development of pathological changes in the bone tissue due to hyperglycemia, it should be noted that many aspects of this problem are still unexplained, and in particular the frequency of bone damage varying in a fairly wide range, the mechanism of reducing bone mineral density that leads to the occurrence of frequent fractures. This article discusses the effects of hyperglycemia on the state of long tubular bones. The goal of our experiment was to study the structural changes in the long tubular bones of senile rats of both sexes with induced hyperglycemia. The period the rats were included into the experiment ranged from 30 to 180 days. During the experiment, various research methods were applied. To perform the histological analysis, we took rat femurs and fixed them in 10% phosphate buffered formalin for 24 hours. Then samples were decalcified in 10% formic acid solution, the materials obtained were embedded in paraffin. The study revealed a gradual thinning of the cortical layer in senile rats that is of great importance due to the fact that there is clinically confirmed increased risk of femoral neck fracture in individuals with type II diabetes. The level of blood glucose throughout the experiment was rather high that indicates the development of chronic hyperglycemia. Having analyzed the data on the effect of hyperglycemia on bone growth parameters and comparing these indicators with the control, we can suggest a delay in longitudinal growth of bones. The results and timing of the experiment show that the degree of changes obtained depends on the duration of the experiment.

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“…Studies have shown low IGF-1 in patients with T1D, which could indirectly affect bone turnover. (13)(14)(15) With impaired endogenous secretion, portal levels of insulin are low in T1D whereas the exogenous insulin results in peripheral hyperinsulinemia. A consequence is that, despite adequate dose of insulin to normalize blood glucose, this does not result in an increase in IGF-1.…”

Section: Introductionmentioning

confidence: 99%

Acute Hyperinsulinemia Alters Bone Turnover in Women and Men With Type 1 Diabetes

et al. 2020

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Type 1 diabetes (T1D) increases fracture risk across the lifespan. The low bone turnover associated with T1D is thought to be related to glycemic control, but it is unclear whether peripheral hyperinsulinemia due to dependence on exogenous insulin has an independent effect on suppressing bone turnover. The purpose of this study was to test the bone turnover marker (BTM) response to acute hyperinsulinemia. Fifty‐eight adults aged 18 to 65 years with T1D over 2 years were enrolled at seven T1D Exchange Clinic Network sites. Participants had T1D diagnosis between age 6 months to 45 years. Participants were stratified based on their residual endogenous insulin secretion measured as peak C‐peptide response to a mixed meal tolerance test. BTMs (CTX, P1NP, sclerostin [SCL], osteonectin [ON], alkaline phosphatase [ALP], osteocalcin [OCN], osteoprotegerin [OPG], osteopontin [OPN], and IGF‐1) were assessed before and at the end of a 2‐hour hyperinsulinemic‐euglycemic clamp (HEC). Baseline ON (r = −0.30, p = .022) and OCN (r = −0.41, p = .002) were negatively correlated with age at T1D diagnosis, but baseline BTMs were not associated with HbA1c. During the HEC, P1NP decreased significantly (−14.5 ± 44.3%; p = .020) from baseline. OCN, ON, and IGF‐1 all significantly increased (16.0 ± 13.1%, 29.7 ± 31.7%, 34.1 ± 71.2%, respectively; all p < .001) during the clamp. The increase in SCL was not significant (7.3 ± 32.9%, p = .098), but the decrease in CTX (−12.4 ± 48.9, p = .058) neared significance. ALP and OPG were not changed from baseline (p = .23 and p = .77, respectively). Baseline ON and SCL were higher in men, but OPG was higher in women (all p ≤ .029). SCL was the only BTM that changed differently in women than men. There were no differences in baseline BTMs or change in BTMs between C‐peptide groups. Exogenous hyperinsulinemia acutely alters bone turnover, suggesting a need to determine whether strategies to promote healthy remodeling may protect bone quality in T1D. © 2020 American Society for Bone and Mineral Research © 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

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Skeletal growth and bone mineral acquisition in type 1 diabetic children; abnormalities of the GH/IGF-1 axis

Cited by 55 publications

References 127 publications

Ti̇p 1 Di̇yabetes Melli̇tus Oluşturulan Siçanlarin Beyni̇nde Lepti̇n Mi̇ktari Azalmaktadir

Ti̇p 1 Di̇yabetes Melli̇tus Oluşturulan Siçanlarin Beyni̇nde Lepti̇n Mi̇ktari Azalmaktadir

Microscopic Structure and Morphometric Parametres of Long Tubular Bones of Senile Rats With Induced Hyperglycemia

Acute Hyperinsulinemia Alters Bone Turnover in Women and Men With Type 1 Diabetes

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