Skeletal Muscle Architecture and Fiber-Type Distribution with the Multiple Bellies of the Mouse Extensor digitorum longus Muscle

Chleboun, Gary S.; Patel, Tina; Lieber, Richard L.

doi:10.1159/000147977

Cited by 36 publications

(36 citation statements)

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“…Mice were anesthetized, and the muscle belly of the fifth digit of the EDL was dissected as described (44). Matched muscles from the same animal transduced with AAV1-LacZ or AAV1-Perm1 were analyzed.…”

Section: Ex Vivo Muscle Contractile Function and Fatigue Resistancementioning

confidence: 99%

“…Time to fatigue was defined as the time it took each muscle to reach 40% of the force generated in the first contraction of the fatigue protocol. Force development was normalized to the muscle physiologic cross-sectional area, calculated as described (44). EDL muscles were also analyzed by Western blot analysis for Perm1 and oxphos protein expression, to confirm efficient transduction and Perm1 function.…”

Section: Ex Vivo Muscle Contractile Function and Fatigue Resistancementioning

confidence: 99%

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Perm1 enhances mitochondrial biogenesis, oxidative capacity, and fatigue resistance in adult skeletal muscle

et al. 2015

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Skeletal muscle mitochondrial content and oxidative capacity are important determinants of muscle function and whole‐body health. Mitochondrial content and function are enhanced by endurance exercise and impaired in states or diseases where muscle function is compromised, such as myopathies, muscular dystrophies, neuromuscular diseases, and age‐related muscle atrophy. Hence, elucidating the mechanisms that control muscle mitochondrial content and oxidative function can provide new insights into states and diseases that affect muscle health. In past studies, we identified Perm1 (PPARGC1‐ and ESRR‐induced regulator, muscle 1) as a gene induced by endurance exercise in skeletal muscle, and regulating mitochondrial oxidative function in cultured myotubes. The capacity of Perm1 to regulate muscle mitochondrial content and function in vivo is not yet known. In this study, we use adeno‐associated viral (AAV) vectors to increase Perm1 expression in skeletal muscles of 4‐wk‐old mice. Compared to control vector, AAV1‐Perm1 leads to significant increases in mitochondrial content and oxidative capacity (by 40‐80%). Moreover, AAV1‐Perm1‐transduced muscles show increased capillary density and resistance to fatigue (by 33 and 31 %, respectively), without prominent changes in fiber‐type composition. These findings suggest that Perm1 selectively regulates mitochondrial biogenesis and oxidative function, and implicate Perm1 in muscle adaptations that also occur in response to endurance exercise.—Cho, Y., Hazen, B. C., Gandra, P. G., Ward, S. R., Schenk, S., Russell, A. P., Kralli, A. Perm1 enhances mitochondrial biogenesis, oxidative capacity, and fatigue resistance in adult skeletal muscle. FASEB J. 30, 674‐687 (2016). http://www.fasebj.org

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Section: Ex Vivo Muscle Contractile Function and Fatigue Resistancementioning

confidence: 99%

Section: Ex Vivo Muscle Contractile Function and Fatigue Resistancementioning

confidence: 99%

Perm1 enhances mitochondrial biogenesis, oxidative capacity, and fatigue resistance in adult skeletal muscle

et al. 2015

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“…To determine the longitudinal stress within the muscle, force was normalized to PCSA, an anatomical parameter whose value is directly proportional to force-generating capacity . L f was determined for each muscle by multiplying L m by the characteristic fifth toe EDL L f :L m ratio of 0.69 (Chleboun et al, 1997). Muscle mass (M in milligrams), muscle density ( = 1.056 g/cm 3 ), fiber pennation angle ( = 11.3°), and L f were used to compute PCSA (in millimeters squared), which is equal to (Mcos)/(L f ).…”

Section: Western Blottingmentioning

confidence: 99%

“…Muscle mass (M in milligrams), muscle density ( = 1.056 g/cm 3 ), fiber pennation angle ( = 11.3°), and L f were used to compute PCSA (in millimeters squared), which is equal to (Mcos)/(L f ). The constants in this formula have been published previously (Mendez and Keys, 1960;Chleboun et al, 1997). Differences between genotypes were detected using the Student's t test.…”

Section: Western Blottingmentioning

confidence: 99%

Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology

Gokhin¹,

Lewis²,

McKeown³

et al. 2010

J Gen Physiol

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“…The myofiber lengths of the muscle belly of the digit II, digit III, digit IV, and digit V in the m. extensor digitorum longus of the mouse are about 0.5, 0.5, 0.55, and 0.6 cm, respectively (Chleboun et al, 1997).…”

mentioning

confidence: 99%

Myofiber Length and Myofiber Arrangement in the Antebrachial and Leg Muscles of Sheep

Konno

Suzuki

2000

Okajimas Folia Anatomica Japonica

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Summary: The myofibers of the skeletal muscle vary in length in muscle and from muscle to muscle. Information about myofiber length and myofiber arrangement is necessary to understand the architecture of muscles and differences in myofiber length in muscles. The purpose of the present study was to determine myofiber lengths, ratio of myofiber length to muscle length, and myofiber arrangement of the antebrachial and leg muscles in the sheep. The myofibers of the antebrachial muscles ranged from 7.3 mm (m. extensor carpi ulneris, bipennate muscle) to 39.6 mm (m. flexor digitorum profundus caput radiale, unipennate muscle) in length. The myofibers of the leg muscles ranged from 10.0 mm (m. flexor digitorum superficialis, multipennate muscle) to 119.2 mm (m. soleus, fusiform muscle) in length. The ratio of myofiber length to muscle length in the fusiform muscle was higher than that in the uni-, bi-, and multipennate muscles. The unipennate muscles tended to have a higher ratio of myofibers length to muscle length than did the bi-and multipennate muscles. No difference in the ratio of myofiber length to muscle length existed between the bipennate muscles and multipennate muscles.The myofibers of the skeletal muscle extend across the entire length of muscle or are shorter than the muscle length. In the human, the myofibers of the m. sartorius range from 1.5 to 30 cm in length, whereas the myofiber lengths of the m. biceps femoris, m. semimembranosus, and m. semitendinosus range from 6 to 11 cm (Barrett, 1962). The myofibers vary in length in muscle and from muscle to muscle.The myofiber length of the distal portion of the m. extensor carpi radialis of the horse is 3.1 cm and of the proximal portion 9.2 cm (Hermanson, 1997). The myofiber lengths of the muscle belly of the digit II, digit III, digit IV, and digit V in the m. extensor digitorum longus of the mouse are about 0.5, 0.5, 0.55, and 0.6 cm, respectively (Chleboun et al., 1997).The elbow extensor muscles of the monkey tend to have shorter myofibers (3.05-4.08 cm) than do the elbow flexor muscles (3.20-6.65 cm) (Roy et al., 1984). The knee extensor muscles of the cat tend to have shorter myofibers (1.92-2.73 cm) than do the knee flexor muscles (2.12-8.45 cm) (Sacks & Roy, 1982). The myofibers of muscle of horse (Hermanson, 1997), mouse (Chleboun et aL, 1997), monkey (Roy et al. , 1984), and cat (Sacks & Roy, 1982) are shorter than the muscle length. The facts imply that the variation in ratio of myofiber length to muscle length is related with the myofiber arrangement.Information about myofiber length and myofiber arrangement is necessary to understand the relation between the architecture of muscles and myofiber length. The purpose of the present study was to determine myofiber lengths, ratio of myofiber length to muscle length, and myofiber arrangement of the antebrachial and leg muscles and to examine correlation between ratio of myofiber length to muscle length and myofiber arrangement in the sheep. Materials and MethodsSix adult sheep were used in this stu...

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Skeletal Muscle Architecture and Fiber-Type Distribution with the Multiple Bellies of the Mouse Extensor digitorum longus Muscle

Cited by 36 publications

References 4 publications

Perm1 enhances mitochondrial biogenesis, oxidative capacity, and fatigue resistance in adult skeletal muscle

Perm1 enhances mitochondrial biogenesis, oxidative capacity, and fatigue resistance in adult skeletal muscle

Tropomodulin isoforms regulate thin filament pointed-end capping and skeletal muscle physiology

Myofiber Length and Myofiber Arrangement in the Antebrachial and Leg Muscles of Sheep

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