Skeletal Muscle Lipidomics as a New Tool to Determine Altered Lipid Homeostasis in Fish Exposed to Urban and Industrial Wastewaters

Abstract

This work applies ultra-high performance liquid chromatography coupled to highresolution mass spectrometry (UPLC-HRMS) to characterize for the first time the lipidome of the skeletal muscle of two fish species (Barbus meridionalis, Squalius laietanus) collected in a Mediterranean River affected by urban and industrial wastewater outflows. The untargeted analysis allowed a clear separation of the lipidome of fish from polluted and reference sites; phosphatidylcholines (PCs), phosphatidylethanolamines (PEs) and their lyso and ether-linked forms were among the distinctive features. The targeted analysis consistently detected a decrease in PC

Metabolomics study of fibroblasts damaged by UVB and BaP

“…LysoPC may affect the degree of oxidation of cells by affecting the lipid composition of the mitochondrial membrane 17 , 18 . The higher the oxidation intensity of PC in cells, the more highly unsaturated PC will be oxidised 35 . The additive effects of BaP and UVB exacerbated the oxidative stress response of HFF-1 cells, and increased LysoPC may have enhanced the inflammatory effect or may have protected damaged cells.…”

Biochemical aspects of susceptibility to stressors in two small cyprinids Squalius laietanus and Barbus meridionalis from the NW Mediterranean

“…Comparisons of baseline enzymatic activities were made in fish sampled in a confirmed reference area, all considering the undeniable anthropogenic fingerprint of NW Mediterranean watercourses (Blanco et al 2019;Carbajal et al 2019;Colin et al 2016b;Maceda-Veiga et al 2013;Marqueño et al 2019;Soler et al 2020). Markers of neurotoxicity signs (AChE), protection (BuChE), energy metabolism (LDH and CS),…”

“…However, the lipidomic composition was more seriously affected in barbel from the downstream sites, which lead the researchers to conclude that this species was more sensitive to obesogens (Marqueño et al 2019). However, fewer pathological responses in blood parameters were seen in chub downstream the same WWTP with respect to the barbel facing metal contamination of a comparable magnitude (Maceda-Veiga et al 2013).…”

Shotgun Lipidomics for the Determination of Phospholipid and Eicosanoid Profiles in Atlantic Salmon (Salmo salar L.) Muscle Tissue Using Electrospray Ionization (ESI)-MS/MS Spectrometric Analysis

“…Meanwhile, high-resolution mass spectrometry (HRMS) has also been effectively employed for lipidomics. For example, Marqueño et al [13] utilized HPLC-HRMS for the first time for the determination of lipid species in the muscle of two fish species: Barbus meridionalis and Squalius laietanus. So far, much of the research on the identification of phospholipids has been carried out using the product-ion analysis mode, which utilizes fragment ions to identify each phospholipid.…”

“…Thus, it is expected that the application of the neutral loss (NL) scanning mode will im-prove the identification process of phospholipids in marine taxa. Indeed, neutral loss (NL) scanning was an effective means for the identification and quantification of phospholipids in marine species such as mussels, clams, and whelks [13][14][15][16].…”