Skeletal muscle proteome of piglets is affected in a muscle-dependent manner by a limiting total sulfur amino acid supply

Conde-Aguilera, Jose Alberto; Lefaucheur, Louis; Gondret, Florence; Delgado-Andrade, Cristina; Mercier, Yves; Tesseraud, Sophie; Milgen, Jaap van

doi:10.1007/s00394-019-02133-y

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…Altogether, this seems to suggest that ULU piglets are breaking down endogenous protein, using the resulting amino acids as an energy source to meet their requirements. A similar mechanism has been reported in the muscle of piglets fed with restricted dietary contents of sulphur amino acids, where restricted piglets increased pyruvate oxidation along with oxidative stress response proteins, as well as heat shock proteins, including HSPB1 43 . Yu et al 44 have reported that pigs fed with low starch and high NDF and ether extract diets suppress mTOR signalling protein synthesis and increase the ubiquitin–proteasome pathway in the skeletal muscle.…”

Section: Discussionsupporting

confidence: 74%

“…These can then be used in the TCA cycle further downstream, where proteins such as IDH3B participate. This is rather interesting given that Conde-Aguilera et al 43 have reported that a lack of amino acid balance leads to fattier carcasses and IDH3B has furthermore been related to fat-accumulation in pigs 49 . The disruption of muscle metabolism homeostasis is additionally supported by the higher abundance of oxidative metabolism proteins (CAT, GSTM3, GLRX3) and heat shock proteins (HSP90, HSPB1 and HSPA1A) in ULU muscle.…”

Section: Discussionmentioning

confidence: 86%

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Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

Ribeiro,

Coelho,

Costa

et al. 2024

Sci Rep

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Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC–MS) approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation may have contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintaining tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects of cell wall degradation during digestion.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 86%

Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

Ribeiro,

Coelho,

Costa

et al. 2024

Sci Rep

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show abstract

“…These results are consistent with other studies showing that changes in feed consumption and growth rate of pigs modify muscle metabolism according to muscle type 31 . For instance, reduced growth rate induced by dietary deficiency in sulphur aminoacids decreased glycolytic metabolism in LM and increased oxidative metabolism and capacity to oxidize fatty acids in rhomboideus muscle, a mixed slow and fast-twitch oxidative muscle 20 , 39 . The current study also indicates an activation of the AMPK pathway in SM in response to hygiene degradation of pig housing conditions.…”

Section: Discussionmentioning

confidence: 99%

Poor hygiene of housing conditions influences energy metabolism in a muscle type-dependent manner in growing pigs differing in feed efficiency

Vincent

Dessauge

Gondret

et al. 2022

Sci Rep

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The ability of pigs to cope with inflammatory challenges may by modified by selection for residual feed intake (RFI), a measure of feed efficiency. In the current study, we evaluated skeletal muscle metabolic responses to degraded hygiene conditions in pigs divergently selected for RFI. At 82 d of age, low RFI and high RFI pigs were housed in either poor or good hygiene conditions. After a 6-week challenge, the poor hygiene conditions induced a decrease in growth performance (P < 0.001) and in plasma IGF-I concentrations (P < 0.003) in both lines. In the slow-twitch oxidative semispinalis muscle, poor hygiene conditions induced a shift towards a more oxidative metabolism and an activation of the AMPK pathway in pigs of both RFI lines. In the fast-twitch glycolytic longississimus muscle, poor hygiene conditions were associated to a less glycolytic metabolism in the HRFI line only. Poor hygiene conditions also increased the protein level of lipidation of microtubule-associated protein 1 light-chain 3β (LC3-II) in both RFI lines, suggesting an activation of the autophagy pathway. Altogether, the data revealed muscle-type specific metabolic adaptations to poor hygiene conditions, which may be related to different strategies to fuel the activated immune system.

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Flash dietary methionine supply over growth requirements in pigs: Multi-facetted effects on skeletal muscle metabolism

Gondret

Floc'H

Batonon-Alavo³

et al. 2021

Animal

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Skeletal muscle proteome of piglets is affected in a muscle-dependent manner by a limiting total sulfur amino acid supply

Cited by 4 publications

References 37 publications

Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

Poor hygiene of housing conditions influences energy metabolism in a muscle type-dependent manner in growing pigs differing in feed efficiency

Flash dietary methionine supply over growth requirements in pigs: Multi-facetted effects on skeletal muscle metabolism

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