Skeletal site‐specific response to ovariectomy in a rat model: change in bone density and microarchitecture

Liu, Xi Ling; Li, Chun Lei; Lu, Wenjia; Cai, Wei; Zheng, Li Wu

doi:10.1111/clr.12360

Cited by 77 publications

(84 citation statements)

References 26 publications

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“…Jiang et al [36] analyzed mandibular trabecular bone in the first and second molar region and also found a negative effect of estrogen deficiency. In the same way, Liu et al [37] found a decrease in BV/TV in maxilla and mandible only 24 weeks after ovariectomy. On the other hand, some authors observed no maxillary or periodontal alterations resulting from ovariectomy [28,38].…”

Section: Discussionmentioning

confidence: 67%

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Esteves

Moraes

Gomes

et al. 2015

Pathology - Research and Practice

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Section: Discussionmentioning

confidence: 67%

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Esteves

Moraes

Gomes

et al. 2015

Pathology - Research and Practice

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“…The ovariectomy (OVX) rat is the most commonly used animal model for evaluating the mechanisms underlying postmenopausal osteoporosis and therapeutic strategies for treating this disease (6)(7)(8)(9). However, compared with rats, mice may be a more effective model as they have a more easily manipulated genome and lower drug doses are required for treatment.…”

Section: Introductionmentioning

confidence: 99%

Age-dependent variations of cancellous bone in response to ovariectomy in C57BL/6J mice

et al. 2018

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Abstract. The ovariectomized (OVX) mouse model has been widely accepted to be suitable for the study of postmenopausal osteoporosis. However, whether C57BL/6J mice, a commonly used genetic background mouse strain, is an appropriate model for postmenopausal osteoporosis remains controversial. The present study investigated the effect of the OVX model on alterations in bone density and microarchitecture in C57BL/6J female mice of different ages. C57BL/6J mice were divided into 8-, 12-and 16-week-old groups (OVX 8 , OVX 12 and OVX 16 ) from the beginning of OVX. At 8 weeks post-surgery, the mice were anesthetized and micro-computed tomography was used to analyze the bone density and microarchitecture. The results revealed that OVX-induced loss of cancellous bone was greatest in OVX 8 , moderate in OVX 12 , and only a weak bone loss was observed in the OVX 16 group when compared with the SHAM 16 control group. In addition, the effect of genetic backgrounds in response to the OVX model were examined. Several other strains of mice, including inbred (BALB/c) and outbred (ICR and Kunming), were used in the present study, all of which were subjected to OVX at 8 weeks of age. The present findings revealed that the highest rate of bone loss was detected in C57BL/6J female mice. In addition, treatment with estrogen (17β-estradiol, 30 µg/kg five times per week) led to a significant increase in bone density in C57BL/6J mice compared with the other strains of mice. Therefore, these results may provide novel insights into the age-and strain-associated effect of OVX on regulating turnover of bone in female mice. The present findings also suggest 8-week-old C57BL/6J mice as an animal model for postmenopausal osteoporosis and preclinical testing of potential therapies for this disease.

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“…5 In rats, the scan typically starts 1 mm away from the growth plate to capture the remodelling, rather than modelling, characteristics. [6][7][8][9] In mice, scans often start at the growth plate, as there is a smaller volume of bone available for analysis. 10 However, scans starting at a distance of up to 0.29 mm from the growth plate have been reported.…”

Section: Scanning Proceduresmentioning

confidence: 99%

“…The size of the scan in the axial direction typically ranges from 1 to 2 mm for mice [10][11][12] and from 2 to 3 mm for rats. 8,9,13 For the vertebra and femoral neck, the entire regions are scanned, and specific analysis volumes are segmented afterwards by the user.…”

Section: Scanning Proceduresmentioning

confidence: 99%

“…The majority of studies on the vertebra examine the entire vertebral body. 11,14,15,19,20 Alternatively, some researchers have specified a volume (for example, 2 mm) centred at the midline, 8 or they have used the growth plate as a landmark. 7,21,22 As the mid-region is sparsely populated with trabecular bone, and the bone volume density is inhomogeneously distributed throughout the vertebral body, it is recommended to analyse the entire volume unless the study question gives cause to focus on a specific subvolume.…”

Section: Trabecular Bonementioning

confidence: 99%

See 1 more Smart Citation

Quantitative analysis of bone and soft tissue by micro-computed tomography: applications to ex vivo and in vivo studies

Campbell¹,

Sophocleous²

2014

BoneKEy Reports

124

106

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Micro-computed tomography (micro-CT) is a high-resolution imaging modality that is capable of analysing bone structure with a voxel size on the order of 10 mm. With the development of in vivo micro-CT, where disease progression and treatment can be monitored in a living animal over a period of time, this modality has become a standard tool for preclinical assessment of bone architecture during disease progression and treatment. For meaningful comparison between micro-CT studies, it is essential that the same parameters for data acquisition and analysis methods be used. This protocol outlines the common procedures that are currently used for sample preparation, scanning, reconstruction and analysis in micro-CTstudies. Scan and analysis methods for trabecular and cortical bone are covered for the femur, tibia, vertebra and the full neonate body of small rodents. The analysis procedures using the software provided by ScancoMedical and Bruker are discussed, and the routinely used bone architectural parameters are outlined. This protocol also provides a section dedicated to in vivo scanning and analysis, which covers the topics of anaesthesia, radiation dose and image registration. Because of the expanding research using micro-CT to study other skeletal sites, as well as soft tissues, we also provide a review of current techniques to examine the skull and mandible, adipose tissue, vasculature, tumour severity and cartilage. Lists of recommended further reading and literature references are included to provide the reader with more detail on the methods described.

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Skeletal site‐specific response to ovariectomy in a rat model: change in bone density and microarchitecture

Abstract: This study for the first time assessed the systemic site-specific bone loss and microarchitecture changes in OVX rat model. It provided valuable information for selecting bone site and observation time in osteoporosis-related study.

Cited by 77 publications

References 26 publications

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Age-dependent variations of cancellous bone in response to ovariectomy in C57BL/6J mice

Quantitative analysis of bone and soft tissue by micro-computed tomography: applications to ex vivo and in vivo studies

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