Skin mucus metabolomics provides insights into the interplay between diet and wound in gilthead seabream (Sparus aurata)

Albaladejo-Riad, Nora; Espinosa, Cristóbal; Esteban, María Ángeles; Lazado, Carlo C.

doi:10.1016/j.fsi.2023.108590

Cited by 3 publications

(6 citation statements)

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“…More recently, metabolic pathway analysis of significantly affected metabolites in wounded and non‐wounded gilthead seabream fed with SF microparticles revealed perturbations in aminoacyl t‐RNA biosynthesis in the skin. In particular, skin wounds resulted in a decreased level of methionine in mucus, whereas SF supplementation increased the level of methionine in skin mucus (Albaladejo‐Riad et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…This was also observed when studying the humoral immune parameters (Albaladejo‐Riad et al, 2022). Perhaps this may be due to the higher amino acid intake of fish fed with SF microparticles, as compared to fish fed with the control diet (Albaladejo‐Riad et al, 2023). In addition, these results show the importance of diet in immunity and in the repair, regeneration and maintenance of tissues and organs.…”

Section: Discussionmentioning

confidence: 99%

“…For the preparation of the diets, the commercial feed (Skretting; protein-56%, fats-15%, fibre-1.2%, ash-9.2%, calcium-2.5%, sodium-0.8% and phosphorus-1.5%) was converted into powder, moistened and mixed with different amounts of silk microparticles: 0% or non-supplemented diet (control), 50 mg of SF microparticles kg −1 supplemented diet (SF50) and 100 mg of SF microparticles kg −1 supplemented diet (SF100). Then, the pellets were remake again (Albaladejo-Riad et al, 2022, 2023. In this research, we tested two concentrations of SF microparticles, 50 and 100 mg per kg of feed, chosen based on similar concentrations reported in other studies involving the administration of micro-or nanoparticles in fish diets, as cited in several papers (Abdel-Tawwab et al, 2018;Espinosa-Ruiz et al, 2017;Sun et al, 2016;Tayemeh et al, 2020).…”

Section: Sf Microparticles and Dietsmentioning

confidence: 99%

“…For this purpose, fish were fed for 30 days with a silk-supplemented diet and then experimental wounds were performed to compromise the welfare status of the fish. The samples were taken from the same fish used in two previous works (Albaladejo-Riad et al, 2022, 2023. In these fish, different samples were taken to analyse different immune parameters present in the skin mucus (local humoral immunity), the macroscopic closure of the wound by taking macroscopic photographs and subsequent image analysis of the same and the processes of skin regeneration using microscopic techniques and expression of genes of interest.…”

Section: Gene Expressionmentioning

confidence: 99%

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Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Albaladejo‐Riad,

Espinosa‐Ruiz,

Esteban

2024

Animal Physiology Nutrition

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Silk fibroin (SF) microparticles were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercially available diet was enriched with varying amount of SF: 0, 50 and 100 mg kg−1 (representing the control, SF50 and SF100 diets respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed on the rest of fish, and they continued to be fed the same diet. At 7 days post‐wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularisation and congestion of blood vessels). In addition, it influences the expression of genes that serve as markers of oxidative stress, endoplasmic reticulum stress and apoptosis. The highest dose of SF (SF100) increased the phagocytic capacity of leucocytes the most, as well as the expression of genes related to blood vessel formation in the liver. Furthermore, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Sf Microparticles and Dietsmentioning

confidence: 99%

Section: Gene Expressionmentioning

confidence: 99%

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Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Albaladejo‐Riad,

Espinosa‐Ruiz,

Esteban

2024

Animal Physiology Nutrition

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“…Plasma antiproteases protect against pathogens by inhibiting the released proteases. Little is known about the influence of fasting on antiprotease activity, and existing studies focus mainly on tissues other than blood or analyze antiprotease activity after a bacterial challenge (54,63,64). However, in a study of Nile tilapia (Oreochromis niloticus) fasted for 21 days, plasma antiprotease activity significantly increased but then decreased during refeeding.…”

Section: Discussionmentioning

confidence: 99%

Gut physiology of rainbow trout (Oncorhynchus mykiss) is influenced more by short-term fasting followed by refeeding than by feeding fishmeal-free diets

Frohn

Peixoto

Terrier

et al. 2023

Preprint

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Supplementing a fishmeal-free diet with yeast extract improves rainbow trout (Oncorhynchus mykiss) growth performance and modulates the hepatic and intestinal transcriptomic response. These effects are often observed in the long term, but are not well documented after short periods of fasting. Fasting for a few days is a common practice in fish farming, especially before handling the fish, such as for short sorting, tank transfers and vaccinations. In the present study, rainbow trout were subjected to a four-day fast and then refed, for eight days, a conventional diet containing fishmeal (control diet) or alternative diets composed of terrestrial animal by-products supplemented or not with a yeast extract. During the refeeding period alone, most of the parameters considered did not differ significantly in response to the different feeds. Only the expression of claudin-15 was upregulated in fish fed the yeast-supplemented diet compared to the control diet. Conversely, fasting followed by refeeding significantly influenced most of the parameters analyzed. In the proximal intestine, the surface area of villi significantly increased and the density of goblet cell tended to decrease during refeeding. Although no distinct plasma immune response or major signs of gut inflammation were observed, some genes involved in the structure, complement pathway, antiviral functions, coagulation and endoplasmic reticulum stress response of the liver and intestine were significantly regulated by refeeding after fasting. These results indicate that short-term fasting, as commonly practiced in fish farming, significantly alters the physiology of the liver and intestine regardless of the composition of the diet.

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Cyrtocarpa edulis fruit and its immunostimulant effect on Almaco Jack Seriola rivoliana: in vitro, in vivo and ex vivo studies

Reyes-Becerril,

Maldonado-García,

López

et al. 2024

Vet Res Commun

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Skin mucus metabolomics provides insights into the interplay between diet and wound in gilthead seabream (Sparus aurata)

Cited by 3 publications

References 73 publications

Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Gut physiology of rainbow trout (Oncorhynchus mykiss) is influenced more by short-term fasting followed by refeeding than by feeding fishmeal-free diets

Cyrtocarpa edulis fruit and its immunostimulant effect on Almaco Jack Seriola rivoliana: in vitro, in vivo and ex vivo studies

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