Skin Prolyl Hydroxylase in Patients With Obstructive Jaundice

Skin Prolylhydroxylase Activity and Wound Healing

“…It is interesting that, although in our pre vious report [6] no correlation could be dem onstrated between plasma total protein or se rum albumin levels and SPHA in jaundiced patients, in the present report, nutritional sta tus measured by serum albumin levels and total lymphocyte count was significantly relat ed with SPHA and wound healing impair ment. On the other hand, we have previously found a significant difference between SPHA in patients with malignant or benign biliary obstruction [6] while the presence of malig nancy did not appear to influence wound healing in the present and other studies [3,18]. Jaundiced patients may have several ab normalities other than hyperbilirubinemia which affect wound healing.…”

“…Either defect in collagen synthesis might account for de layed wound healing [2,3], Although other authors [12] propose the measurement of hydroxyproline accumula tion in the wound space as the most accurate technique to assess wound healing, this meth od has limitations, such as the fact that it is a relatively invasive technique for human stud ies and that results are widely scattered, re flecting the degree of both inter-and intrapa tient variation [13]. The measurement of tis sue prolylhydroxylase is a reasonable alterna tive to measuring collagen synthesis, as dem onstrated in studies on wound healing [2,3,6] and other alterations of fibrinogenesis [14,15], Moreover, it has been argued [ 16] that the amount of collagen present in a wound space is not critical in the development of wound strength, which is due specifically to the crosslinking within collagen fibrils, a process not assessed by the measurement of SPHA. Like other authors [13,17], we contend that the measurement of SPHA is not used in this study to determine ultimate wound healing strength, but rather to measure the capacity of wound healing, as shown in jaundiced pa tients [2,3,6].…”

“…The measurement of tis sue prolylhydroxylase is a reasonable alterna tive to measuring collagen synthesis, as dem onstrated in studies on wound healing [2,3,6] and other alterations of fibrinogenesis [14,15], Moreover, it has been argued [ 16] that the amount of collagen present in a wound space is not critical in the development of wound strength, which is due specifically to the crosslinking within collagen fibrils, a process not assessed by the measurement of SPHA. Like other authors [13,17], we contend that the measurement of SPHA is not used in this study to determine ultimate wound healing strength, but rather to measure the capacity of wound healing, as shown in jaundiced pa tients [2,3,6].…”

“…Collagen synthesis and wound healing. However, the measurement of prolylhydroxylase in tissues is a reasonable alternative to measuring collagen synthesis per se since the activity of this enzyme parallels the rate of synthesis of colla gen, which reflects collagen turnover [2,3].…”

Toxicity of Bilirubin and Bilirubin Diglucuronide to Rat Tissue Culture Fibroblasts

“…Similarly jaundiced pa tients frequently have multiple problems or complications each one of which could be suspected of contributing towards poor wound healing [Armstrong et al, 1984;Bayer and Ellis, 1976;Ellis and Heddle, 1977;Greaney et al, 1979;Irvin et al, 1978;Karakantzas, 1975;Lee, 1972;Than, 1976]. In addition, jaundiced patients may have predominantly raised serum and tissue lev els of conjugated bilirubin or unconjugated bilirubin depending on the cause of the jaun dice.…”