Skin resistance to UVB-induced oxidative stress and hyperpigmentation by the topical use of <i>Lactobacillus helveticus </i>
NS8-fermented milk supernatant

Rong, Jingjing; Shan, Chuang; Liu, Shuzhan; Zheng, Houfeng; Liu, Chen; Liu, Ming; Jin, Feng; Wang, Li

doi:10.1111/jam.13506

Cited by 29 publications

(33 citation statements)

References 37 publications

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“…Lactobacillus is a strain of lactic acid bacteria (LAB) found in the gastrointestinal tract, and these are the most commonly used probiotics in foods. The studies on LAB are usually focused on anti-inflammation and intestinal immune system, with a range of health-promoting effects having been reported for the genus Lactobacillus [18][19][20]. Furthermore, fermentation with probiotics potentially increases the number of microorganisms in the diet and can result in new salutary compounds, which can be beneficial to the body [21].…”

Section: Introductionmentioning

confidence: 99%

Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes

Kang

Hong

Kang³

et al. 2020

JFB

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Ultraviolet (UV) exposure triggers the abnormal production of reactive oxygen (ROS) species and the expression of matrix metalloproteinases (MMPs) that are responsible for photoaging. Probiotics are widely used in healthcare and for immune enhancement. One probiotic, Lactobacillus buchneri is found in Kimchi. This study was aimed at assessing the anti-photoaging effect of plant extracts fermented with L. buchneri (PELB) to develop functional cosmetics. We investigated the anti-photoaging effect of PELB in a UVB-induced photoaging in vitro model and selected effective extracts using the elastase inhibition assay, ELISA for Type I procollagen and collagenase-1, and quantitative real time PCR. Normal human dermal fibroblasts and epidermal keratinocytes were pre-treated with PELB and exposed to UVB. We found that PELB decreased elastase activity and increased type I collagen expression in a UVB-induced photoaging in vitro model. In addition, PELB greatly reduced collagenase activity and MMP mRNA levels in a UVB-induced photoaging in vitro model. Furthermore, PELB promoted the expression of moisture factor and anti-oxidant enzymes in a UVB-induced photoaging in vitro model. These results indicated that the PELB could be potential candidates for the protective effects against UVB-induced photoaging. Overall, these results suggest that PELB might be useful natural components of cosmetic products.

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Section: Introductionmentioning

confidence: 99%

Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes

Kang

Hong

Kang³

et al. 2020

JFB

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“…Subsequently, the complete medium is replaced with 100 µL of PBS, and the cells are exposed to 300 mJ/cm 2 UVB irradiation. The medium is immediately replaced with complete medium and RES, OXYR, or RES + OXYR and incubated at 37 °C with 5% CO 2 for 24 h. The intracellular tyrosinase activity of the PIG1 cells are estimated by measuring the l -DOPA oxidation capacity according to the method of Rong et al [ 21 ]. The PIG1 cells are washed twice with PBS, added to 50 µL of 1% Triton X-100, and then incubated at −80 °C for 30 min, allowed to thaw at room temperature, added to 10 µL of freshly prepared l -DOPA (0.25%, Solarbio, Beijing, China), and incubated at 37 °C for 120 min.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Promotion on Tyrosinase Inhibition by Antioxidants

et al. 2018

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When exposed to ultraviolet radiation, the human skin produces profuse reactive oxygen species (ROS), which in turn activate a variety of biological responses. Mounting ROS levels activate tyrosinase by mobilizing α-melanocyte-stimulating hormone in the epidermis and finally stimulates the melanocytes to produce melanin. Meanwhile, the Keap1-Nrf2/ARE pathway, which removes ROS, is activated at increased ROS levels, and antioxidant compounds facilitates the dissociation of Nrf2. In this study, we explored the possible suppressing effects of antioxidant compounds and tyrosine inhibitors on melanin formation and the promotory effects of these compounds on ROS scavenging. The antioxidant activity of glabridin (GLA), resveratrol (RES), oxyresveratrol (OXYR), and phenylethylresorcinol (PR) were investigated via the stable free radical 2,2-diphenyl-1-picrylhydrazyl method. The inhibitory effects of the four compounds and their mixtures on tyrosinase were evaluated. l-Tyrosine or 3-(3,4-dihydroxyphenyl)-l-alanine (l-DOPA) was used as a substrate. The results showed that all mixtures did not exhibit synergistic effects with the l-tyrosine as a substrate, suggesting that l-tyrosine is not suitable as a substrate. However, the mixtures of “GLA:RES,” “GLA:OXYR,” “OXYR:RES,” and “PR:RES” demonstrated synergistic effects (CI < 0.9, p < 0.05), whereas “GLA:RES” and “PR:OXYR” indicated an additive effect (0.9 ditive1, p < 0.05). Furthermore, we used a molecular docking strategy to study the interactions of the four compounds with tyrosinase and l-DOPA. The molecular docking result is consistent with that of the experiment. Finally, we selected RES + OXYR and used PIG1 cells to verify whether OXYR synergistically promotes RES activity on tyrosinase. The two agents had a synergistic inhibitory effect on tyrosinase activity. These results provided a novel synergistic strategy for antioxidants and tyrosinase inhibitors, and this strategy is useful in skin injury treatment.

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“…Western blotting was performed according to a previously described protocol . The primary antibodies included those against caspase‐3 (1:1000, 9665, Cell Signaling), cleaved caspase‐3 (1:1000, 9664, Cell Signaling), p‐IκBα (1:1000, 2859, Cell Signaling), IκBα (1:1000, 4814, Cell Signaling), and β‐actin (1:2000, sc‐37642, Santa Cruz Biotechnology, Dallas, TX, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative polymerase chain reaction assays were performed according to a previously described protocol . The primer sequences are shown in Table S1.…”

Section: Methodsmentioning

confidence: 99%

Single probiotic supplement suppresses colitis‐associated colorectal tumorigenesis by modulating inflammatory development and microbial homeostasis

Rong

Liu

et al. 2018

J of Gastro and Hepatol

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Background and Aim Chronic inflammation is a major contributor to the initiation and progression of cancers. Lactobacillus helveticus NS8, which was originally separated from fermented koumiss, exhibited anti‐inflammatory functions in our prior studies. In this study, NS8 was investigated for its potential to prevent colitis‐associated colorectal cancer (CAC). Methods The protective effects of NS8 against CAC was explored by employing the azoxymethane plus dextran sodium sulfate‐induced carcinogenesis mouse model. The prevalences of T cells expressing specific inflammatory cytokines were measured by flow cytometry at the early stage of CAC. Inflammatory modulation by NS8 was also tested in the Caco2‐Raw264.7 cell co‐culture system. The alternations in the intestinal microbiota following the health–inflammation–cancer sequence were analyzed by 16S rDNA sequencing. Results Oral intake of NS8 lactobacilli clearly reduced tumor number and the degree of hyperplasia. The increased proliferation of enterocytes at the early stage of CAC was significantly suppressed by NS8, while the level of apoptosis was elevated. The anticancer effects of NS8 were associated with its anti‐colitis outcomes before tumor formation. NS8 significantly suppressed the activation of NF‐κB and upregulated the anti‐inflammatory cytokine IL‐10. Further analysis revealed the marked downregulation of IL‐17‐producing T cells by NS8. Furthermore, NS8 modulated intestinal dysbiosis by promoting beneficial commensal microbes while suppressing cancer‐associated microbes. Notably, Bacteroides acidifaciens was the most sensitive commensal bacteria to NS8 intervention. Conclusion These results provide insight into the protective effects of L. helveticus NS8 against colorectal cancer.

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Skin resistance to UVB-induced oxidative stress and hyperpigmentation by the topical use of Lactobacillus helveticus NS8-fermented milk supernatant

Abstract: Our findings indicate the potential of cell-free fermented products of lactic acid bacteria in topical photoprotection.

Cited by 29 publications

References 37 publications

Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes

Anti-Photoaging Effect of Plant Extract Fermented with Lactobacillus buchneri on CCD-986sk Fibroblasts and HaCaT Keratinocytes

Synergistic Promotion on Tyrosinase Inhibition by Antioxidants

Single probiotic supplement suppresses colitis‐associated colorectal tumorigenesis by modulating inflammatory development and microbial homeostasis

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