SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC

Gao, Qi; Zhang, Guiji; Zheng, Yaqiu; Yang, Yi; Chang, Chen; Xia, Jie; Liang, Li; Lei, Chong; Hu, Yuan; Cai, Xue-Fei; Zhang, Wenlu; Tang, Hua; Chen, Yaxi; Huang, Ailong; Wang, Kai; Tang, Ni

doi:10.1038/s41418-019-0399-1

Cited by 88 publications

(80 citation statements)

References 42 publications

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“…The corresponding reaction is that cancer cells will rise levels of antioxidant proteins (such as TXNRD1), which can detoxify the enhancive ROS to maintain redox balance and anti-apoptosis [50]. Indeed, as described in some hepatocellular carcinoma cases, TXNRD1 was elevated to control ROS level and maintain the tumorigenesis [51,52]. These previous studies suggested that reduced TXNRD1 strengthened the anticancer treatment, which showed a consistent trend in our research.…”

Section: Discussionsupporting

confidence: 88%

Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1

Song

Wang

et al. 2020

Preprint

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Background: The morbidity and mortality of esophageal squamous cell carcinoma (ESCC) remains stubbornly high, in spite of emerging new diagnoses methods. The role of circular RNAs (circRNAs) in ESCC progression is still in need of more exploration. Methods: In this research, we gathered 36 patients’ ESCC tissues to analyze the expression of circ0120816, miR-1305 and TXNRD1. KYSE450 and KYSE510 cells were used to conduct the transfection. Aiming to verify our hypothesis, qRT-PCR, RNase R treatment, nuclear extraction, luciferase reporter assay, RNA immunoprecipitation assay, CCK-8, BrdU, cell adhesion, caspase 3 activity assay were used. Results: Circ0120816, upregulated in ESCC, acted as a sponge for miR-1305. Circ0120816 combined miR-1305 to enhance cell viability, proliferation adhesion and repress cell apoptosis in ESCC cell lines. On the contrary, miR-1305 exerted reversed effect in ESCC cells through decreasing TXNRD1. Conclusions: This research demonstrated that circ0120816 promoted ESCC development by competitive binding miR-1305 which could increase the expression of TXNRD1.

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Section: Discussionsupporting

confidence: 88%

Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1

Song

Wang

et al. 2020

Preprint

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“…SELENOP mRNA was consistently significantly decreased in hepatocellular carcinoma (HCC) in a study conducted in Xi'an, China ( 33 ), and TXNRD1 expression level was also increased in data from the GEO and TCGA databases ( 34 ). TXNRD1 mRNA was significantly increased in HCC patients from Hong Kong ( 35 ), Zhengzhou ( 34 ), Chongqing ( 36 , 37 ), and Guangzhou ( 38 ), China. High levels of TXNRD1 were associated with poor prognosis ( 34-38 ), and Auranofin, a TXNRD1 inhibitor, was shown to inhibit tumour growth in a mouse model, and in HCC cells ( 35-37 ).…”

Section: Discussionmentioning

confidence: 99%

Potential relationship between the selenoproteome and cancer

2020

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The role of the selenoproteome, which is the collection of all proteins containing selenium in an organism, in cancer development, growth and progression requires further investigation, due to the importance of selenium in both cancer and immune system function. Data about the selenoproteome, including its differential expression, single nucleotide variations, copy number variations, methylation, pathways and overall survival (OS) in five leading types of cancer are available from the GSCALite website. Subsequent to the analysis of these datasets, it was revealed that there was increased expression of GPX3 in stomach adenocarcinoma and lung squamous cell carcinoma, SELENOV in oesophageal carcinoma, GPX8 and GPX4 in colon adenocarcinoma, TXNRD1 and SEPHS1 in hepatocellular carcinoma and GPX8 in lung adenocarcinoma were associated with poor survival. Decreased gene expression of SELENOP was indicated in liver hepatocellular carcinoma and GPX3, and SELENOW, SELENOK, SELENBP1 and SECISBP2 in lung adenocarcinoma were associated with a poor prognosis. OS data suggested that hypermethylation of GPX4 in colon adenocarcinoma, GPX8 in lung squamous cell carcinoma, GPX1 in stomach adenocarcinoma and GPX3 in lung adenocarcinoma was associated with low survival, as is hypomethylation of GPX5 in lung adenocarcinoma. The selenoproteome is heterogeneous, especially in its effect on the OS of patients with cancer. The present study demonstrated that the roles of GPX4 in colon adenocarcinoma, SCLY and SELENOV in oesophageal carcinoma, SEPHS1 in liver hepatocellular carcinoma, SELENOK in lung cancer, as well as SELENOM and SELENOW in stomach adenocarcinoma requires further research. The present study may lead to the identification of novel biomarkers or potential therapeutic targets for use in the treatment of cancers, such as colon adenocarcinoma, oesophageal carcinoma, liver hepatocellular carcinoma, lung cancer and stomach adenocarcinoma.

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“…was elevated to control the ROS level and maintain the tumorigenesis [73,74]. These previous studies suggested that reduced TXNRD1 strengthened anticancer treatments.…”

Section: Discussionmentioning

confidence: 93%

Circ0120816 acts as an oncogene of esophageal squamous cell carcinoma via inhibiting miR-1305 and releasing TXNRD1

Song

Wang

et al. 2020

Preprint

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Background: Circular RNAs (circRNAs) have been discovered to participate in the carcinogenesis of multiple cancers. However, the role of circRNAs in esophageal squamous cell carcinoma (ESCC) progression is yet to be properly understood. This research aimed to investigate and understand the mechanism used by circRNAs to regulate ESCC progression.Methods: Bioinformatics analysis was first performed to screen dysregulated circRNAs and differentially expressed genes in ESCC. The ESCC tissue samples and adjacent normal tissue samples utilized in this study were obtained from 36 ESCC patients. All the samples were subjected to qRT-PCR analysis to identify the expression of TXNRD1, circRNAs, and miR-1305. Luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay were later conducted to verify the existing relationship among circ0120816, miR-1305 and TXNRD1. CCK-8, BrdU, cell adhesion, cell cycle, western blot and caspase 3 activity assays were also employed to evaluate the regulation of these three biological molecules in ESCC carcinogenesis. To evaluate the effect of circ0120816 on ESCC tumor growth and metastasis, the xenograft mice model was constructed. Results: Experimental investigations revealed that circ0120816 was the highest upregulated circRNA in ESCC tissues and that this non-coding RNA acted as a miR-1305 sponge in enhancing cell viability, cell proliferation, and cell adhesion as well as repressing cell apoptosis in ESCC cell lines. Moreover, miR-1305 was observed to exert a tumor-suppressive effect in ESCC cells by directly targeting and repressing TXNRD1. It was also noticed that TXNRD1 could regulate cyclin, cell adhesion molecule, and apoptosis-related proteins. Furthermore, silencing circ0120816 was found to repress ESCC tumor growth and metastasis in vivo.Conclusions: This research confirmed that circ0120816 played an active role in promoting ESCC development by targeting miR-1305 and upregulating oncogene TXNRD1.

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SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC

Cited by 88 publications

References 42 publications

Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1

Circ0120816 Acts As An Oncogene of Esophageal Squamous Cell Carcinoma via Inhibiting miR-1305 and Releasing TXNRD1

Potential relationship between the selenoproteome and cancer

Circ0120816 acts as an oncogene of esophageal squamous cell carcinoma via inhibiting miR-1305 and releasing TXNRD1

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