Sleep interruption associated with house staff work schedules alters circadian gene expression

Fang, Ming; Ohman‐Strickland, Pamela; Kelly‐McNeil, Kathie; Kipen, Howard M.; Crabtree, Benjamin F.; Lew, Jenny; Zarbl, Helmut

doi:10.1016/j.sleep.2015.06.011

Cited by 19 publications

(25 citation statements)

References 34 publications

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“…After at least a week on a day-oriented schedule (3 wk according to their work roster), clock genes PER1, PER2, and PER3 had significantly higher gene expression at 10:00 compared with 19:30. These results are consistent with prior studies on individuals living on conventional schedules (30,43,44). After 7 consecutive night shifts, all 3 PER genes lost this morning/evening difference in PBMCs.…”

Section: Discussionsupporting

confidence: 92%

Disruption of central and peripheral circadian clocks in police officers working at night

Koshy¹,

Cuesta²,

Boudreau³

et al. 2019

FASEB j.

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Working atypical schedules leads to temporal misalignments between a worker's rest‐activity cycle and their endogenous circadian system. Several studies have reported disturbed centrally controlled rhythms, but little is known on shift workers' peripheral clocks. Here, we assessed central clock markers, urinary 6‐sulfatoxymelatonin and salivary cortisol, and clock gene expression in 2 peripheral clocks, oral mucosa cells and peripheral blood mononuclear cells (PBMCs), in 11 police officers. Before working 7 consecutive nights, officers' centrally controlled rhythms were aligned to a day‐oriented schedule. These rhythms were partially realigned to the shifted schedule and dampened after a week working nights. For peripheral clocks at baseline, Period (PER)1–3 and nuclear receptor subfamily 1, group D, member 1 (REV‐ERBα) in oral mucosa cells had a significant mRNA peak in the afternoon, whereas in PBMCs, higher PER1–3 expression was observed at 10:00 compared with 19:30. After a week working nights, PER1–3 and REV‐ERBα expression in oral mucosa cells lost rhythmicity, and in PBMCs, the morning/evening difference observed at baseline was lost. To our knowledge, this is the first study to demonstrate the disruption of several peripheral clocks in real shift workers. Molecular circadian disturbances are believed to have important clinical implications for the occurrence of shift work–associated medical disorders.—Koshy, A., Cuesta, M., Boudreau, P., Cermakian, N., Boivin, D. B. Disruption of central and peripheral circadian clocks in police officers working at night. FASEB J. 33, 6789–6800 (2019). http://www.fasebj.org

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Section: Discussionsupporting

confidence: 92%

Disruption of central and peripheral circadian clocks in police officers working at night

Koshy¹,

Cuesta²,

Boudreau³

et al. 2019

FASEB j.

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“…Significantly, the rhythmicity of Per2 mRNA levels in the blood depended on the period of activity of the subjects, therefore, it was higher in the morning for day shift workers, similar to the general population, but higher in the evening for night shift workers (Fang, et al 2015). This shift in Per2 levels was coincident with testosterone synthesis, suggesting strong association between androgen production and the expression of Per2.…”

Section: Relation Between the Circadian Clock And Androgensmentioning

confidence: 84%

WOMEN IN CANCER THEMATIC REVIEW: Circadian rhythmicity and the influence of ‘clock’ genes on prostate cancer

Kiss

Ghosh

2016

Endocrine-Related Cancer

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The androgen receptor (AR) plays a key role in the development and progression of prostate cancer (CaP). Since the mid-1990s, reports in the literature pointed out higher incidences of CaP in some select groups, such as airline pilots and night shift workers in comparison to those working regular hours. The common finding in these “high-risk” groups was that they all experienced a deregulation of the body’s internal circadian rhythm. Here we discuss how the circadian rhythm affects androgen levels and modulates CaP development and progression. Circadian rhythmicity of androgen production is lost in CaP patients, with the clock genes Per1 and Per2 decreasing, and Bmal1 increasing, in these individuals. Periodic expression of the clock genes was restored upon administration of the neurohormone melatonin, thereby suppressing CaP progression. Activation of the melatonin receptors and the AR antagonized each other, and therefore the tumor suppressive effects of melatonin and the clock genes were most clearly observed in the absence of androgens, that is, in conjunction with androgen deprivation therapy (ADT). In addition, a large-scale study found that high-dose radiation was more effective in CaP patients when it was delivered before 5PM, compared to those delivered after 5PM, suggesting that the therapy was more effective when delivered in synchrony with the patient’s circadian clock. As CaP patients are shown to become easily resistant to new therapies, perhaps circadian delivery of these therapeutic agents or delivery in conjunction with melatonin and its novel analogs should be tested to see if they prevent this resistance.

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“…It is well documented that circadian rhythms regulate physiological and biological processes involved in the sleep-wake cycle, body temperature, metabolism, alertness, and cognition1234. Akashi et al .…”

Section: Discussionmentioning

confidence: 99%

“…Circadian clock genes regulate the daily rhythms of physiology and behavior such as locomotor activity, feeding time, sleep-wake cycle, body temperature, metabolism, alertness, and cognition in all mammals including humans1234. Scheduled feeding and exercise in mice can entrain peripheral circadian rhythms under light-dark conditions5.…”

mentioning

confidence: 99%

Positive association between physical activity and PER3 expression in older adults

Takahashi

Haraguchi

Tahara

et al. 2017

Sci Rep

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The circadian clock regulates many physiological functions including physical activity and feeding patterns. In addition, scheduled exercise and feeding themselves can affect the circadian clock. The purpose of the present study was to investigate the relationship between physical/feeding activity and expression of clock genes in hair follicle cells in older adults. Twenty adult men (age, 68 ± 7 years, mean ± SE) were examined in this cross-sectional study. Prior to hair follicle cell collection, the participants were asked to wear a uniaxial accelerometer for one week. The timings of breakfast, lunch, and dinner were also recorded. Hair follicle cells were then collected over a 24 h period at 4 h intervals. The amplitude of PER3 expression was positively correlated with moderate and vigorous physical activity (r = 0.582, p = 0.007) and peak oxygen uptake (r = 0.481, p = 0.032), but these correlations were not observed for NR1D1 or NR1D2. No association was noted between meal times and the amplitude or the acrophase for any of these three clock genes. These findings suggest that rhythmic expression of the circadian clock gene PER3 is associated with the amount of daily physical activity and physical fitness in older adults.

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Sleep interruption associated with house staff work schedules alters circadian gene expression

Cited by 19 publications

References 34 publications

Disruption of central and peripheral circadian clocks in police officers working at night

Disruption of central and peripheral circadian clocks in police officers working at night

WOMEN IN CANCER THEMATIC REVIEW: Circadian rhythmicity and the influence of ‘clock’ genes on prostate cancer

Positive association between physical activity and PER3 expression in older adults

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