Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis

Kuriu, Satoru; Kadonosono, Tetsuya; Kizaka‐Kondoh, Shinae; Ishida, Tadashi

doi:10.3390/mi11050480

Cited by 11 publications

(15 citation statements)

References 31 publications

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“…However, this method requires specialized laboratories and equipment [101][102][103]. Due to its ability to guarantee gases permeability, polydimethylsiloxane (PDMS) is the most used material for making microfluidic devices [104]. In addition, PDMS are biocompatible, easy to make, and are low cost.…”

Section: Microfluidic Devicesmentioning

confidence: 99%

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

et al. 2020

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Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority of promising preclinical drugs have no or weak efficacy in real patients with tumors, thereby delaying the discovery of successful therapeutics. This is because 2D culture lacks cell–cell contacts and natural tumor microenvironment, important in tumor signaling and drug response, thereby resulting in a reduced malignant phenotype compared to the real tumor. In this sense, three-dimensional (3D) cultures of cancer cells that better recapitulate in vivo cell environments emerged as scientifically accurate and low cost cancer models for preclinical screening and testing of new drug candidates before moving to expensive and time-consuming animal models. Here, we provide a comprehensive overview of 3D tumor systems and highlight the strategies for spheroid construction and evaluation tools of targeted therapies, focusing on their applicability in cancer research. Examples of the applicability of 3D culture for the evaluation of the therapeutic efficacy of nanomedicines are discussed.

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Section: Microfluidic Devicesmentioning

confidence: 99%

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

et al. 2020

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“…This system can replicate the in vivo tumour microvasculature and guarantees the permeability of gases. continuous release of oxygen and nutrients [122][123][124][125]. Limitations: Requires specialised equipment; post culture recovery can be difficult; difficult to precisely control the flow speed [122][123][124][125]…”

Section: Microfluidic Platformsmentioning

confidence: 99%

“…Advantages:Can mimic the tumour vasculature; high throughput drug screening; continuous release of oxygen and nutrients[122][123][124][125].Limitations: Requires specialised equipment;post culture recovery can be difficult; difficult to precisely control the flow speed[122][123][124][125] Matrix encapsulationCancers 2022, 14, x FOR PEER REVIEW 12 of 37Microfluidic platformsdistribution of oxygen and nutrients and the elimination of waste[117]. This system can replicate the in vivo tumour microvasculature and guarantees the permeability of gases.…”

mentioning

confidence: 99%

3D Bioprinting: An Enabling Technology to Understand Melanoma

Fernandes

Vyas

Lim

et al. 2022

Cancers

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Melanoma is a potentially fatal cancer with rising incidence over the last 50 years, associated with enhanced sun exposure and ultraviolet radiation. Its incidence is highest in people of European descent and the ageing population. There are multiple clinical and epidemiological variables affecting melanoma incidence and mortality, such as sex, ethnicity, UV exposure, anatomic site, and age. Although survival has improved in recent years due to advances in targeted and immunotherapies, new understanding of melanoma biology and disease progression is vital to improving clinical outcomes. Efforts to develop three-dimensional human skin equivalent models using biofabrication techniques, such as bioprinting, promise to deliver a better understanding of the complexity of melanoma and associated risk factors. These 3D skin models can be used as a platform for patient specific models and testing therapeutics.

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“…Therefore, the components of MIC should be mechanically assembled by sealing with screws. For the tight mechanical sealing, PDMS was a good material from the point of softness and adhesion to EPOX structures [27].…”

Section: Mic 251 Design Of the Micmentioning

confidence: 99%

Microfluidic Device Using Mouse Small Intestinal Tissue for the Observation of Fluidic Behavior in the Lumen

2021

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The small intestine has the majority of a host’s immune cells, and it controls immune responses. Immune responses are induced by a gut bacteria sampling process in the small intestine. The mechanism of immune responses in the small intestine is studied by genomic or histological techniques after in vivo experiments. While the distribution of gut bacteria, which can be decided by the fluid flow field in the small intestinal tract, is important for immune responses, the fluid flow field has not been studied due to limits in experimental methods. Here, we propose a microfluidic device with chemically fixed small intestinal tissue as a channel. A fluid flow field in the small intestinal tract with villi was observed and analyzed by particle image velocimetry. After the experiment, the distribution of microparticles on the small intestinal tissue was histologically analyzed. The result suggests that the fluid flow field supports the settlement of microparticles on the villi.

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Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis

Cited by 11 publications

References 31 publications

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

3D Bioprinting: An Enabling Technology to Understand Melanoma

Microfluidic Device Using Mouse Small Intestinal Tissue for the Observation of Fluidic Behavior in the Lumen

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