Slk19p is necessary to prevent separation of sister chromatids in meiosis I

Kamieniecki, Rebecca J.; Shanks, Robert M. Q.; Dawson, Dean

doi:10.1016/s0960-9822(00)00723-5

Cited by 53 publications

(59 citation statements)

References 40 publications

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“…The phenotype resembled that observed in the dyad mutant of Arabidopsis in which the majority of female meiocytes divide singly to give a dyad followed by an arrest in further development of the female gametophyte (Siddiqi et al, 2000). Single division meiosis has also been described for the spo12, spo13 and slk19 mutants of yeast (Klapholz and Esposito, 1980;Zeng and Saunders, 2000;Kamieniecki et al, 2000).…”

Section: Discussionmentioning

confidence: 88%

TheDUETgene is necessary for chromosome organization and progression during male meiosis inArabidopsisand encodes a PHD finger protein

et al. 2003

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Progression through the meiotic cell cycle is an essential part of the developmental program of sporogenesis in plants. The duet mutant of Arabidopsis was identified as a male sterile mutant that lacked pollen and underwent an aberrant male meiosis. Male meiocyte division resulted in the formation of two cells instead of a normal tetrad. In wild type, male meiosis extends across two successive bud positions in an inflorescence whereas in duet, meiotic stages covered three to five bud positions indicating defective progression. Normal microspores were absent in the mutant and the products of the aberrant meiosis were uni- to tri-nucleate cells that later degenerated, resulting in anthers containing largely empty locules. Defects in male meiotic chromosome organization were observed starting from diplotene and extending to subsequent stages of meiosis. There was an accumulation of meiotic structures at metaphase 1, suggesting an arrest in cell cycle progression. Double mutant analysis revealed interaction with dyad, a mutation causing chromosome cohesion during female meiosis. Cloning and molecular analysis of DUET indicated that it potentially encodes a PHD-finger protein and shows specific expression in male meiocytes. Taken together these data suggest that DUET is required for male meiotic chromosome organization and progression.

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Section: Discussionmentioning

confidence: 88%

TheDUETgene is necessary for chromosome organization and progression during male meiosis inArabidopsisand encodes a PHD finger protein

et al. 2003

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“…We tested the ability of these deleted versions of Slk19p to activate the FEAR network by taking advantage of the fact that FEAR mutants form only dyads after meiosis (Klapholz and Esposito 1980;Kamieniecki et al 2000;Zeng and Saunders 2000;Buonomo et al 2003;Marston et al 2003). We created diploid strains homozygous for each deletion allele and scored for the ability of the strain to form tetrads after meiosis.…”

Section: Resultsmentioning

confidence: 99%

Slk19p of Saccharomyces cerevisiae Regulates Anaphase Spindle Dynamics Through Two Independent Mechanisms

Havens

Gardner

Kamieniecki³

et al. 2010

Genetics

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Slk19p is a member of the Cdc-14 early anaphase release (FEAR) pathway, a signaling network that is responsible for activation of the cell-cycle regulator Cdc14p in Saccharomyces cerevisiae. Disruption of the FEAR pathway results in defects in anaphase, including alterations in the assembly and behavior of the anaphase spindle. Many phenotypes of slk19D mutants are consistent with a loss of FEAR signaling, but other phenotypes suggest that Slk19p may have FEAR-independent roles in modulating the behavior of microtubules in anaphase. Here, a series of SLK19 in-frame deletion mutations were used to test whether Slk19p has distinct roles in anaphase that can be ascribed to specific regions of the protein. Separation-offunction alleles were identified that are defective for either FEAR signaling or aspects of anaphase spindle function. The data suggest that in early anaphase one region of Slk19p is essential for FEAR signaling, while later in anaphase another region is critical for maintaining the coordination between spindle elongation and the growth of interpolar microtubules.

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“…meiosis I spindle but not so much as to permit the assembly of prereplicative complexes onto the DNA, which would result in a new S phase. It has been shown that exit from meiosis I requires only the FEAR pathway, since mutants in this pathway are impaired in meiosis I spindle disassembly, whereas MEN inactivation does not affect exit from the first meiotic division (Kamieniecki et al 2000;Buonomo et al 2003;Marston et al 2003;Kamieniecki et al 2005). Notably, even though chromosome segregation during meiosis I is not efficient in FEAR network mutants, they are able to progress through meiosis II and form two spores (dyads) instead of four (tetrads) (Sharon and Simchen 1990;Marston et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Cdc15 Is Required for Spore Morphogenesis Independently of Cdc14 in Saccharomyces cerevisiae

et al. 2007

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In Saccharomyces cerevisiae exit from mitosis requires the Cdc14 phosphatase to reverse CDK-mediated phosphorylation. Cdc14 is released from the nucleolus by the Cdc14 early anaphase release (FEAR) and mitotic exit network (MEN) pathways. In meiosis, the FEAR pathway is essential for exit from anaphase I. The MEN component Cdc15 is required for the formation of mature spores. To analyze the role of Cdc15 during sporulation, a conditional mutant in which CDC15 expression was controlled by the CLB2 promoter was used. Cdc15-depleted cells proceeded normally through the meiotic divisions but were unable to properly disassemble meiosis II spindles. The morphology of the prospore membrane was aberrant and failed to capture the nuclear lobes. Cdc15 was not required for Cdc14 release from the nucleoli, but it was essential to maintain Cdc14 released and for its nucleo-cytoplasmic transport. However, cells carrying a CDC14 allele with defects in nuclear export (Cdc14-DNES) were able to disassemble the spindle and to complete spore formation, suggesting that the Cdc14 nuclear export defect was not the cause of the phenotypes observed in cdc15 mutants. MEIOSIS is a specialized cell division that produces the haploid cells needed for sexual reproduction, and the completion of meiosis is normally coupled to differentiation programs that produce gametes. In Saccharomyces cerevisiae, sporulation is initiated when diploid cells are grown in the absence of nitrogen and the presence of a nonfermentable carbon source such as acetate (Esposito and Klapholtz 1981). When triggered to enter the sporulation program, cells exit the mitotic cycle from the G1 phase. This is followed by one round of S phase and two meiotic divisions that generate four haploid nuclei, which are packaged into individual spores. As a consequence of this process, four haploid gametes (spores) are formed in the cytoplasm of the mother cell (ascus) (reviewed by Neiman 2005). At the onset of meiosis II, the spindle pole body (SPB) is modified by the incorporation of several meiosis-specific proteins, which together make up the meiosis II outer plaque (Knop and Strasser 2000;Bajgier et al. 2001;Nickas et al. 2003). This change in composition converts the function of the cytoplasmic face of the SPB from microtubule nucleation to membrane nucleation. The process of spore construction requires the de novo assembly of two cellular structures, the prospore membrane (PSM) that is generated around the daughter nuclei to create prospores and a protective spore wall that surrounds the prospores. Once the meiosis II outer plaque of the SPB has been assembled, it becomes a site for the docking and coalescence of secretory vesicles to form flattened double-membrane sheets termed PSMs (Moens 1971;Neiman 1998). These nascent PSMs expand during meiosis II, and at the time of nuclear division each PSM completely engulfs the nuclear lobe to which it is anchored via the SPB. At the completion of meiosis II, each PSM fuses with itself so that each daughter nucleus (and assoc...

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Slk19p is necessary to prevent separation of sister chromatids in meiosis I

Abstract: Our results suggest that Slk19p is essential at the centromere of meiotic chromosomes to prevent the premature separation of sister chromatids at meiosis I.

Cited by 53 publications

References 40 publications

TheDUETgene is necessary for chromosome organization and progression during male meiosis inArabidopsisand encodes a PHD finger protein

TheDUETgene is necessary for chromosome organization and progression during male meiosis inArabidopsisand encodes a PHD finger protein

Slk19p of Saccharomyces cerevisiae Regulates Anaphase Spindle Dynamics Through Two Independent Mechanisms

Cdc15 Is Required for Spore Morphogenesis Independently of Cdc14 in Saccharomyces cerevisiae

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