SlNAC2 overexpression in Arabidopsis results in enhanced abiotic stress tolerance with alteration in glutathione metabolism

Borgohain, Pankaj; Saha, Bedabrata; Agrahari, Raj Kishan; Chowardhara, Bhaben; Sahoo, Smita; Vyver, Christell van der; Panda, Sanjib Kumar

doi:10.1007/s00709-019-01368-0

Cited by 40 publications

(14 citation statements)

References 63 publications

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“…Moreover, ectopic expression of SlNAC2 conferred both salt (200 mM) and drought (20% PEG) tolerance up to 10 days in transgenic Arabidopsis lines, which is correlated with the lower accumulation of ROS. In addition, the transcriptomic abundance GSH metabolizing genes was also observed in transgenic lines, leading to increased GSH synthesis and lesser oxidative damage [236].…”

Section: Genetic Manipulation Of Asa-gsh Pathway and Its Role In Amentioning

confidence: 99%

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

et al. 2019

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Reactive oxygen species (ROS) generation is a usual phenomenon in a plant both under a normal and stressed condition. However, under unfavorable or adverse conditions, ROS production exceeds the capacity of the antioxidant defense system. Both non-enzymatic and enzymatic components of the antioxidant defense system either detoxify or scavenge ROS and mitigate their deleterious effects. The Ascorbate-Glutathione (AsA-GSH) pathway, also known as Asada–Halliwell pathway comprises of AsA, GSH, and four enzymes viz. ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase, play a vital role in detoxifying ROS. Apart from ROS detoxification, they also interact with other defense systems in plants and protect the plants from various abiotic stress-induced damages. Several plant studies revealed that the upregulation or overexpression of AsA-GSH pathway enzymes and the enhancement of the AsA and GSH levels conferred plants better tolerance to abiotic stresses by reducing the ROS. In this review, we summarize the recent progress of the research on AsA-GSH pathway in terms of oxidative stress tolerance in plants. We also focus on the defense mechanisms as well as molecular interactions.

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Section: Genetic Manipulation Of Asa-gsh Pathway and Its Role In Amentioning

confidence: 99%

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

et al. 2019

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“…Antioxidant enzymes such as SOD, CAT and POD are the main enzymes for scavenging excess MDA in cells. [50]. Our results showed that after low temperature treatment, the activities of SOD and CAT, the key enzymes of MDA scavenging in chloroplasts, were the highest in the antisense expression lines and the lowest in the overexpression lines, resulting in the highest MDA content in the CtCP1 overexpression lines and the lowest in the antisense inhibition lines (Fig.…”

Section: Physiological Mechanism Of Ctcp1 Regulating Low Temperature Resistance Of Carthamus Tinctoriusmentioning

confidence: 54%

Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress

Hong¹,

Lv²,

Zhang³

et al. 2021

Preprint

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BackgroundCysteine protease (CP) plays an important role in plant senescence. In this study, the whole genome evolution information of CPs was analyzed by using safflower, and the function of CtCP1 under l abiotic stress was analyzed.Results25 CPs members were identified in the safflower genome and divided into 9 subfamilies. Gene structure analysis showed that the possible evolutionary conservatism and functional similarity of the same family members. qRT-PCR at different florescence showed that the expression of CPs gene was the highest in the decline period, and CtCP1 gene changed significantly under abiotic stress. We cloned the qRT-PCR of CtCP1, at different florescence and stress, which showed that the expression of CtCP1 was the highest in the decline stage and low temperature. In order to study the function of CtCP1 gene, we obtained the overexpression CtCP1 line (OE) and the inhibition CtCP1 expression line (Anti) in Arabidopsis thaliana. The results of transgenic lines under low temperature stress showed that inhibition of CtCP1 expression enhanced the resistance of Carthamus tinctorius to low temperature, and overexpression of CtCP1 weakened the resistance of Carthamus tinctorius to low temperature.ConclusionWe have identified the cysteine protease genome of safflower and CtCP1 gene expression under abiotic stress

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“…Glutathione metabolism plays a key role in cellular defense (Noctor et al, 2002;Ball et al, 2004). In glutathione metabolism, glutathione can be oxidized to glutathione disulfide, and glutathione disulfide is again reduced to glutathione by glutathione reductase (Gong et al, 2018;Borgohain et al, 2019). And, dehydroascorbate (DHA) is reduced to ascorbic acid (Asc) by dehydroascorbate reductase (DHAR) in the presence of reduced glutathione, which in turn is regenerated by glutathione reductase (Chang et al, 2017).…”

Section: Analysis Of Secondary Metabolites Biosynthesis and Glutathione Metabolism In Response To Drought Stressmentioning

confidence: 99%

Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)

Zhao

Cui

et al. 2021

Front. Genet.

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Drought is one of the major abiotic stress factors limiting peanut production. It causes the loss of pod yield during the pod formation stage. Here, one previously identified drought-tolerant cultivar, “L422” of peanut, was stressed by drought (35 ± 5%) at pod formation stage for 5, 7, and 9 days. To analyze the drought effects on peanut, we conducted physiological and transcriptome analysis in leaves under well-watered (CK1, CK2, and CK3) and drought-stress conditions (T1, T2, and T3). By transcriptome analysis, 3,586, 6,730, and 8,054 differentially expressed genes (DEGs) were identified in “L422” at 5 days (CK1 vs T1), 7 days (CK2 vs T2), and 9 days (CK3 vs T3) of drought stress, respectively, and 2,846 genes were common DEGs among the three-time points. Furthermore, the result of weighted gene co-expression network analysis (WGCNA) revealed one significant module that was closely correlated between drought stress and physiological data. A total of 1,313 significantly up-/down-regulated genes, including 61 transcription factors, were identified in the module at three-time points throughout the drought stress stage. Additionally, six vital metabolic pathways, namely, “MAPK signaling pathway-plant,” “flavonoid biosynthesis,” “starch and sucrose metabolism,” “phenylpropanoid biosynthesis,” “glutathione metabolism,” and “plant hormone signal transduction” were enriched in “L422” under severe drought stress. Nine genes responding to drought tolerance were selected for quantitative real-time PCR (qRT-PCR) verification and the results agreed with transcriptional profile data, which reveals the reliability and accuracy of transcriptome data. Taken together, these findings could lead to a better understanding of drought tolerance and facilitate the breeding of drought-resistant peanut cultivars.

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SlNAC2 overexpression in Arabidopsis results in enhanced abiotic stress tolerance with alteration in glutathione metabolism

Cited by 40 publications

References 63 publications

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress

Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress

Transcriptome and Co-expression Network Analyses Reveal Differential Gene Expression and Pathways in Response to Severe Drought Stress in Peanut (Arachis hypogaea L.)

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