Slow controlled-rate freezing of human in vitro matured oocytes: effects on maturation rate and kinetics and parthenogenetic activation

Versieren, Karen; Heindryckx, Björn; O’Leary, Thomas; Croo, Ilse De; Abbeel, E. Van den; Gerris, Jan; Sutter, Petra De

doi:10.1016/j.fertnstert.2011.06.060

Cited by 13 publications

(13 citation statements)

References 35 publications

(41 reference statements)

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“…On the other hand, Zhang et al [32] suggested that, as cryopreservation method, vitrification of immature oocytes better preserves the microtubule organization and reduces the cytoskeletal spindle damage. In another recent study, Versieren et al [33] reported that cryopreserved GV oocytes showed decreased and delayed maturation after warming but activation potential was not affected. In this study, oocytes at different developmental stages of maturation (GV, MI, or MII) and oocytes that failed to fertilize after IVF or ICSI were cryopreserved by slow freezing.…”

Section: Discussionmentioning

confidence: 96%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

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Purpose This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification. Methods A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2). Results Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p<0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p<0.05). Conclusion IVM procedure is more efficient when it is performed before oocyte vitrification.

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Section: Discussionmentioning

confidence: 96%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

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“…Baka et al [29] employed conventional epifluorescence to demonstrate improved spindle and chromosome integrity with slow-freezing at the germinal vesicle stage when compared with the metaphase II stage. Versieren et al [34] reported decreased maturation when slow-freezing at the germinal vesicle stage, with no differences in parthenogenetic activation and development for immature oocytes slow-frozen either before or after IVM. Goud et al [33] showed lower survival, but similar maturation and cleavagestage development, in immature oocytes compared with oocytes that underwent slow-freeze after IVM.…”

Section: Previous Research: In-vitro Maturation Then Cryopreservationmentioning

confidence: 99%

In-vitro maturation of germinal vesicle and metaphase I eggs prior to cryopreservation optimizes reproductive potential in patients undergoing fertility preservation

Lee

Sekhon

Grunfeld

et al. 2014

Current Opinion in Obstetrics &Amp; Gynecology

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Conservation of retrieved immature oocytes after vaginal oocyte retrieval has become a major concern for patients, as they strive to maximize the reproductive viability of all oocytes obtained during treatment. Oocyte cryopreservation is important for patients at risk of ovarian cancer, elective fertility preservation and potentially for ovum donation. The superior maturation rate of germinal vesicle and metaphase I oocytes in the fresh IVM vs. postthaw groups provides strong impetus to mature oocytes to the metaphase II stage prior to cryopreservation.

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“…When considering the results of cryopreservation of the oocytes in IVM, it is necessary to pay attention to whether the reference literature performed the slow‐freezing method or vitrification . Recent reports have not concluded whether it is preferable to cryopreserve immature oocytes at the GV or MII stage . Vitrification results in more normally formed spindles than the slow‐freezing method .…”

Section: Cryopreservation Of Oocytes For Fertility Preservationmentioning

confidence: 99%

In vitro maturation of human immature oocytes for fertility preservation and research material

Shirasawa

Terada

2017

Reprod Medicine & Biology

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AimIn recent years, the importance of fertility preservation (FP) has increased. In vitro maturation (IVM), an important technique in FP, has started to be used in the clinic, but controversies persist regarding this technique. Here, a survey of IVM for FP is provided.MethodsBased on a literature review, the applications of FP, methods of FP, IVM of oocytes that had been collected in vivo and ex vivo, maturation of oocytes after IVM for FP, cryopreservation of oocytes for FP, explanation of the procedures to patients, and recent research on FP using IVM were investigated.ResultsAlthough IVM for FP remains controversial, the application of FP is expected to expand. Depending on the age and disease status of the patient, various methods of oocyte collection and ovarian stimulation, as well as various needle types and aspiration pressures, have been reported. The maturation rate of IVM in FP ranges widely and requires optimization in the future. In regard to cryopreservation for matured oocytes, the vitrification method is currently recommended.ConclusionRegarding FP for patients with cancer, the treatment of cancer is prioritized; thus, the time and use of medicines are often constrained. As several key points regarding IVM remain unclear, well‐designed and specific counseling for patients is necessary.

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Slow controlled-rate freezing of human in vitro matured oocytes: effects on maturation rate and kinetics and parthenogenetic activation

Cited by 13 publications

References 35 publications

In-vitro maturation of human oocytes: before or after vitrification?

In-vitro maturation of human oocytes: before or after vitrification?

In-vitro maturation of germinal vesicle and metaphase I eggs prior to cryopreservation optimizes reproductive potential in patients undergoing fertility preservation

In vitro maturation of human immature oocytes for fertility preservation and research material

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