2009
DOI: 10.1016/j.fertnstert.2008.01.076
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Slow cooling of human oocytes: ultrastructural injuries and apoptotic status

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Cited by 112 publications
(64 citation statements)
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“…We observed this circumstance qualitatively in oocytes cryopreserved with CRSC protocols involving the use of 0.1 or 0.3 mol/l sucrose as a constituent of the freezing solution [19]. Similar findings were described by Gualtieri et al [24]. Consistent with this, we found that oocytes cryopreserved by a CRSC protocol including ethylene glycol (EG) as an intracellular CPA [23] or by vitrification [39] were also affected by a loss of CG.…”
Section: Discussionsupporting
confidence: 87%
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“…We observed this circumstance qualitatively in oocytes cryopreserved with CRSC protocols involving the use of 0.1 or 0.3 mol/l sucrose as a constituent of the freezing solution [19]. Similar findings were described by Gualtieri et al [24]. Consistent with this, we found that oocytes cryopreserved by a CRSC protocol including ethylene glycol (EG) as an intracellular CPA [23] or by vitrification [39] were also affected by a loss of CG.…”
Section: Discussionsupporting
confidence: 87%
“…Images were further enlarged on the PC screen, in order to easily recognize and count CG. Values were expressed in number of CG per 10 μm of the oocyte linear surface profile [24]. Statistical data were shown as mean±SD.…”
Section: Discussionmentioning
confidence: 99%
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“…Mitochondrial membrane potential (ΔΨm) is the foundation of energy metabolism in cells. It is a sensitive index of cell damage because it is easily to be influenced by the environment [8]. JC-1 is an ideal probe to detect ΔΨm, whose emission of red and green fluorescence only relies on ΔΨm and won't be affected by other factors such as the oocyte size and the mitochondria number [9].…”
Section: Introductionmentioning
confidence: 99%
“…A significant DNA fragmentation has been found in vitrified bovine oocytes [38]. It has been reported that the process of oocyte vitrification can induce profound modifications to mitochondria, cortical granules, microvilli, oolemma, smooth endoplasmic reticulum (SER) and cytoskeleton [39][40][41][42][43]. Therefore, taken together, it appears that the quality of blastocysts is detrimentally affected by both maternal reproductive age and vitrification/ warming procedures.…”
Section: Discussionmentioning
confidence: 99%