Small RNAs and extracellular vesicles: New mechanisms of cross-species communication and innovative tools for disease control

Cai, Qiang; He, Baoye; Weiberg, Arne; Buck, Amy H.; Jin, Hailing

doi:10.1371/journal.ppat.1008090

Cited by 142 publications

(110 citation statements)

References 83 publications

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“…EVs also participate in plant-fungi communication (Cai et al, 2019 ). In 2011, based on the ultrastructural characterization of the haustorium-forming phytopathogen Golovinomyces orontii , it was observed MVB-like structures fusing with (or budding off from) the fungal plasma membrane and also membrane-bound vesicles in the extra-haustorial matrix, suggesting the occurrence of vesicular release outside of the haustorial cell wall during plant-fungi interactions (Micali et al, 2011 ).…”

Section: Cell-to-cell Communication Mediated By Evsmentioning

confidence: 99%

“…Despite the already mentioned open questions (Margolis and Sadovsky, 2019 ; Raposo and Stahl, 2019 ), substantial progress has been made to elevate EVs to the position of important mediators of intercellular and interkingdom communication processes, given their ability to transfer bioactive components (Maas et al, 2017 ; van Niel et al, 2018 ; Cai et al, 2019 ; Mathieu et al, 2019 ; Correa et al, 2020 ) and surmount biological barriers, including the blood-brain barrier (BBB) (Alvarez-Erviti et al, 2011 ).…”

Section: Evs As Vehicles For the Delivery Of Biologically Active Molementioning

confidence: 99%

“…In a sense, bacterial, fungal and parasite RNA sequences were identified in human body fluids (Beatty et al, 2014 ), suggesting that RNA-associated EVs could represent biomarkers of infectious diseases (Beatty et al, 2014 ; Hoy et al, 2014 ). EV-based strategies for the control of infectious diseases have also been suggested, including the use of artificial vesicle-protected RNA antifungal strategies, and other RNA-based techniques for host-induced gene silencing (Cai et al, 2019 ).…”

Section: Evs As Vehicles For the Delivery Of Biologically Active Molementioning

confidence: 99%

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Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives

Rizzo

Rodrigues

Janbon

2020

Front. Cell. Infect. Microbiol.

109

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Extracellular vesicles (EVs) have garnered much interest in the cell biology and biomedical research fields. Many studies have reported the existence of EVs in all types of living cells, including in fifteen different fungal genera. EVs play diverse biological roles, from the regulation of physiological events and response to specific environmental conditions to the mediation of highly complex interkingdom communications. This review will provide a historical perspective on EVs produced by fungi and an overview of the recent discoveries in the field. We will also review the current knowledge about EV biogenesis and cargo, their role in cell-to-cell interactions, and methods of EV analysis. Finally, we will discuss the perspectives of EVs as vehicles for the delivery of biologically active molecules.

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Section: Cell-to-cell Communication Mediated By Evsmentioning

confidence: 99%

Section: Evs As Vehicles For the Delivery Of Biologically Active Molementioning

confidence: 99%

Section: Evs As Vehicles For the Delivery Of Biologically Active Molementioning

confidence: 99%

See 1 more Smart Citation

Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives

Rizzo

Rodrigues

Janbon

2020

Front. Cell. Infect. Microbiol.

109

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“…Whether BFV miRNAs may have additional functions independent of the posttranscriptional control of cellular gene expression is currently unknown, but taking their high intracellular concentrations in chronically BFV-infected cells into account, this appears possible. The high intracellular concentrations of miR-BF1-5p and miR-BF2-5p [8] may be a prerequisite (or at least an advantage) for extracellular functions (e.g., in the form of free or exosome-associated miRNAs with local or systemic effects) [61]. For instance, it has recently been shown that under conditions of bacterial sepsis, the extracellular form of the murine and human miR-130b-3p is released into the blood and interacts, thereby attenuating the pro-inflammatory function of CIRP (cold-inducible RNA binding protein), a ligand of TLR4-mediated innate inflammatory signaling [62,63].…”

Section: Discussionmentioning

confidence: 99%

Functional Analyses of Bovine Foamy Virus-Encoded miRNAs Reveal the Importance of a Defined miRNA for Virus Replication and Host–Virus Interaction

Cao

Stricker

Hotz‐Wagenblatt

et al. 2020

Viruses

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In addition to regulatory or accessory proteins, some complex retroviruses gain a repertoire of micro-RNAs (miRNAs) to regulate and control virus–host interactions for efficient replication and spread. In particular, bovine and simian foamy viruses (BFV and SFV) have recently been shown to express a diverse set of RNA polymerase III-directed miRNAs, some with a unique primary miRNA double-hairpin, dumbbell-shaped structure not known in other viruses or organisms. While the mechanisms of expression and structural requirements have been studied, the functional importance of these miRNAs is still far from understood. Here, we describe the in silico identification of BFV miRNA targets and the subsequent experimental validation of bovine Ankyrin Repeat Domain 17 (ANKRD17) and Bax-interacting factor 1 (Bif1) target genes in vitro and, finally, the suppression of ANKRD17 downstream genes in the affected pathway. Deletion of the entire miRNA cassette in the non-coding part of the U3 region of the long terminal repeats attenuated replication of corresponding BFV mutants in bovine cells. This repression can be almost completely trans-complemented by the most abundant miRNA BF2-5p having the best scores for predicted and validated BFV miRNA target genes. Deletion of the miRNA cassette does not grossly affect particle release and overall particle composition.

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“…However, systemic transfer, which involves molecules crossing multiple barriers in the body, is not the only way that dietary RNA could affect health beyond serving as fuel 4 . Dietary-RNA carriers have access to the epithelial and immune-surveillance cells of the alimentary tract.…”

mentioning

confidence: 99%