Small Round Structured Virus Associated With an Outbreak of Acute Gastroenteritis in Chiba, Japan

Kasuga, K.; Tokieda, Masayoshi; Ohtawara, Misao; Utagawa, Etsuko; Yamazaki, Shudo

doi:10.7883/yoken1952.43.111

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…The capsid molecular mass of 49.4 kDa, was lower than expected from the 57–63 kDa range reported for human enteric caliciviruses with SRSV morphology [12,13,17–20]. It was also lower than the 55 kDa molecular mass of the in vitro translated capsid protein of the Jena virus, the only other bovine enteric calicivirus with a known capsid molecular mass [8].…”

Section: Discussionmentioning

confidence: 55%

Characterisation of the bovine enteric calici-like virus, Newbury agent 1

Dastjerdi

Snodgrass²,

Bridger

2000

FEMS Microbiology Letters

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The bovine enteric calici-like virus, Newbury agent 1 (NA1) was characterised to determine if it is a member of the Caliciviridae and to establish its antigenic relationship to the established bovine enteric calicivirus Newbury agent 2 (NA2). Solid phase immune electron microscopy (SPIEM) allowed quantification of NA1 virions and identification of faecal samples with optimal virus levels. NA1 particles were 36.6 nm in diameter, had an indefinite surface structure resembling that of human small round structured viruses (SRSVs), and a buoyant density of 1.34 g ml(-1). A single capsid protein of 49.4 kDa was detected by Western blotting in purified NA1 preparations prepared from post-infection but not pre-infection faecal samples and with post- but not pre-infection sera. NA1 was antigenically unrelated to the bovine enteric calicivirus NA2 by SPIEM. These properties were consistent with classification of NA1 within the Caliciviridae but demonstrated heterogeneity in the capsid composition of bovine enteric caliciviruses.

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Section: Discussionmentioning

confidence: 55%

Characterisation of the bovine enteric calici-like virus, Newbury agent 1

Dastjerdi

Snodgrass²,

Bridger

2000

FEMS Microbiology Letters

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“…NLV used for the expression of the capsid protein was the prototype Chiba virus, NLV/Chiba407/1987/JP strain, that was derived from a stool collected during an oyster-associated outbreak of gastroenteritis in Chiba prefecture, Japan, in 1987[Kasuga et al, 1990. Purification of the virion, preparation of the RNA and cDNA synthesis were performed as described previously [Utagawa et al, 1994].…”

Section: Expression Of Recombinant CV Capsids (Rcv)mentioning

confidence: 99%

“…The present study describes the development of an antigen ELISA based on hyperimmune sera to baculovirus-expressed capsid protein of Chiba virus (CV), a Japanese strain in genogroup I [Kasuga et al, 1990;Utagawa et al, 1994]. To evaluate the specificity of the ELISA, we determined the nucleotide sequences of ELISA-positive strains and phylogenetically analyzed their genotype.…”

Section: Introductionmentioning

confidence: 99%

Serotype-specific antigen ELISA for detection of Chiba virus in stools

et al. 2000

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Chiba virus (CV), a Norwalk-like virus (NLV), was first identified as a cause of oyster-associated outbreak of gastroenteritis that occurred in Chiba prefecture, Japan, in 1987. An enzyme-linked immunosorbent assay (ELISA), based on hyperimmune antisera to recombinant baculovirus-expressed capsid proteins of CV (rCV), was developed to detect CV antigen in stools. No cross-reactions were observed with other enteric viruses including enteroviruses, rotaviruses, astroviruses, or enteric adenoviruses. The ELISA was used to screen 101 stools collected from 16 oyster-associated outbreaks of acute gastroenteritis. Twelve stools (11.9%) from seven outbreaks were positive for CV antigen. Ten rCV ELISA-positive strains were confirmed by RT-PCR and nucleotide sequencing. ELISA-positive strains showed 96-100% nucleotide sequence identity to each other, though they were obtained nine years apart. Phylogenetic analysis demonstrated that all ten strains clustered with the prototype CV in genogroup I viruses. We concluded that the antigen ELISA described in this study is highly type-specific, and that this method should be useful for epidemiological surveys of Chiba virus infections.

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“…Hyperimmune antisera to the purified recombinant SeV (rSeV) were prepared in rabbits (four doses of 250 g of protein/dose with Freund's complete adjuvant). The specificity of rabbit hyperimmune antisera to rSeV or rCV (14,15) was tested in parallel with indirect ELISAs. The ELISA method employed was identical to the ELISA for rNV (2,11).…”

mentioning

confidence: 99%

Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses

Kobayashi

Sakae

Suzuki

et al. 2000

Journal of Clinical Microbiology

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The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.

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Small Round Structured Virus Associated With an Outbreak of Acute Gastroenteritis in Chiba, Japan

Cited by 8 publications

References 12 publications

Characterisation of the bovine enteric calici-like virus, Newbury agent 1

Characterisation of the bovine enteric calici-like virus, Newbury agent 1

Serotype-specific antigen ELISA for detection of Chiba virus in stools

Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses

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