2007
DOI: 10.1016/j.tibtech.2007.08.014
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Smart thermoresponsive coatings and surfaces for tissue engineering: switching cell-material boundaries

Abstract: The smart thermoresponsive coatings and surfaces that have been explicitly designed for cell culture are mostly based on poly(N-isopropylacrylamide) (PNIPAAm). This polymer is characterized by a sudden precipitation on heating, switching from a hydrophilic to a hydrophobic state. Mammalian cells cultured on such thermoresponsive substrates can be recovered as confluent cell sheets, while keeping the newly deposited extracellular matrix intact, simply by lowering the temperature and thereby avoiding the use of … Show more

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Cited by 289 publications
(233 citation statements)
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“…A review on cell sheet engineering by Kikuchi and Okano also showed the release of cell sheets from PNIPAAm films and the dependence on film thickness [140][141][142]. Films 20-30 nm show the best cell adhesion and detachment [140][141][142].…”
Section: Filmsmentioning
confidence: 99%
“…A review on cell sheet engineering by Kikuchi and Okano also showed the release of cell sheets from PNIPAAm films and the dependence on film thickness [140][141][142]. Films 20-30 nm show the best cell adhesion and detachment [140][141][142].…”
Section: Filmsmentioning
confidence: 99%
“…Another advantage of the cell sheet engineering approach is that the laminated layers resulted in rapid neovascularization unlike transplantation of thick constructs with cell-seeded scaffolds. A recent report reviewed other advantages of the cell sheet engineering approach such as easy harvesting procedure, possibility of sutureless transplantation, and even distribution of cells in the sheet without mass transfer problem [31]. Nevertheless, one disadvantage of this approach is that it is difficult to construct thick tissues as each layer is around 30 lm thick.…”
Section: Scaffolding Approaches In Tissue Engineeringmentioning
confidence: 99%
“…Confluent cultured cell sheets are conventionally removed enzymatically or mechanically, but these two methods damage the cells and the extracellular matrix they may be producing (Canavan et al 2005). However, thermoresponsive coatings which change from hydrophobic to hydrophilic at lowered temperatures can release cultured cells and extracellular matrix as an intact sheet upon cooling (da Silva et al 2007). This method known as "thermal liftoff," results in undamaged sheets that maintain the ability to adhere if transferred onto another substrate (da Silva et al 2007) and opens the possibility of stacking sheets to create a three-dimensional product.…”
Section: Tissue Culturementioning
confidence: 99%
“…However, thermoresponsive coatings which change from hydrophobic to hydrophilic at lowered temperatures can release cultured cells and extracellular matrix as an intact sheet upon cooling (da Silva et al 2007). This method known as "thermal liftoff," results in undamaged sheets that maintain the ability to adhere if transferred onto another substrate (da Silva et al 2007) and opens the possibility of stacking sheets to create a three-dimensional product. Lam et al (2009) have presented a method for detaching culture as a confluent sheet from a non-adhesive micropatterned surface using the biodegradation of selective attachment protein laminin.…”
Section: Tissue Culturementioning
confidence: 99%
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